Name: ReadiUse™ Preactivated APC-iFluor® 800 Tandem
Brand: AAT Bioquest
SKU: 2572
Price: 415 USD
Availability: InStock

ReadiUse™ Preactivated APC-iFluor® 800 Tandem

ReadiUse™ Preactivated APC-iFluor® 800 Tandem is a ready-to-conjugate near-infrared fluorescent tandem dye for labeling antibodies and other proteins for multicolor and spectral flow cytometry.

Fluorescence Detection: Excitation at 651 nm and emission at 820 nm.
Conjugation Chemistry: Buccutite™ FOL/MTA chemistry enables protein conjugation through amino-group modification.
High Conjugation Yield: Higher yields than conventional SMCC-based chemistry.
Ready-to-Use: Includes preactivated dye and Buccutite™ MTA reagent.
Spectral Compatibility: Near-infrared emission minimizes overlap with common fluorophores.

Our preactivated APC was premodified with our Buccutite™ FOL (provided). Your antibody (or other proteins) is modified with our Buccutite™ MTA (provided as free sample) to give MTA-modified protein (such as antibody). The MTA-modified protein readily reacts with FOL-modified APC (provided) to give the desired APC-antibody conjugate in much higher yield than the SMCC chemistry. In addition our preactivated APC reacts with MTA-modified biopolymers at much lower concentrations than the SMCC chemistry.

Protocol

SDS

Catalog	Size	Price	Quantity
2572	1 mg	Price

Overview

Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. Our APC-iFluor® 800 Tandem is a unique fluorophore with an IR emission developed for spectral flow cytometry. Its emission spectrum does not overlap with any of the common fluorophores, making a unique color for multicolor applications in flow cytometry. ReadiUse™ Preactivated APC-iFluor® 800 Tandem is an activated APC protein, and can be easily conjugated to antibodies with much higher conjugation yield than the conventional APC tandems.

References

View all 2 references: Citation Explorer

Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism.

Authors:

Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90

Cy7PE and Cy7APC: bright new probes for immunofluorescence.

Authors:

Roederer, M and Kantor, A B and Parks, D R and Herzenberg, L A

Journal:

Cytometry (1996): 191-7

Spectral properties

Absorbance (nm)	804
Extinction coefficient (cm ^-1 M ^-1)	700000
Excitation (nm)	651
Emission (nm)	820

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on October 14, 2026

A: ReadiUse™ Preactivated APC-iFluor® 800 Tandem	1 vial (1 mg)
B: Buccutite™ MTA	1 vial (100 µg)
C: Spin Desalting Column	Not Included