ReadiUse™ Preactivated PE

ReadiUse™ Preactivated PE is a ready-to-conjugate fluorescent protein label for preparing antibody and protein conjugates used in flow cytometry.

Fluorescence Detection: Excitation at 565 nm and emission at 574 nm.
Conjugation Chemistry: Buccutite™ FOL/MTA chemistry enables protein conjugation through amino-group modification.
High Conjugation Yield: Higher yields than conventional SMCC-based chemistry.
Ready-to-Use: Includes preactivated PE and Buccutite™ MTA reagent.
Efficient Labeling: Supports conjugation at lower protein concentrations than SMCC chemistry.

<p>Our preactivated PE was premodified with our Buccutite™ FOL (provided). Your antibody (or other proteins) is modified with our Buccutite™ MTA (provided as free sample) to give MTA-modified protein (such as antibody). The MTA-modified protein readily reacts with FOL-modified PE (provided) to give the desired PE-antibody conjugate in much higher yield than the SMCC chemistry. In addition our preactivated PE reacts with MTA-modified biopolymers at much lower concentrations than the SMCC chemistry.</p>

Protocol

SDS

Catalog	Size	Price	Quantity
2560	1 mg	Price

Overview

R-Phycoerythrin (PE) is isolated from red algae. Its primary absorption peak is at 565 nm with secondary peaks at 496 and 545 nm. AAT Bioquest offers this preactivated PE to facilitate the PE conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated PE is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.

References

View all 96 references: Citation Explorer

Imaging Flow Cytometry for Phylogenetic and MorphologicallyBased Functional Group Clustering of a Natural Phytoplankton Community over 1 Year in an Urban Pond.

Authors:

Dunker, Susanne

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2020)

In Vivo Photodynamic Therapy With a Lipophilic Zinc(II) Phthalocyanine Inhibits Colorectal Cancer and Induces a Th1/CD8 Antitumor Immune Response.

Authors:

Chiarante, Nicolás and Duhalde Vega, Maite and Valli, Federico and Zotta, Elsa and Daghero, Hellen and Basika, Tatiana and Bollati-Fogolin, Mariela and García Vior, María C and Marino, Julieta and Roguin, Leonor P

Journal:

Lasers in surgery and medicine (2020)

Purification of Live Stem-Cell-Derived Islet Lineage Intermediates.

Authors:

Rasouli, Niloofar and Melton, Douglas A and Alvarez-Dominguez, Juan R

Journal:

Current protocols in stem cell biology (2020): e111

Application Settings versus Fixed Photomultiplier Tube Voltages for Optimal Cytometer Stability over Time, and Effect on Reusing a Compensation Matrix.

Authors:

Andersen, Morten Nørgaard and Skovbo, Anni and Petersen, Charlotte Christie and Hokland, Marianne

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2020)

Improved Flow Cytometric Light Scatter Detection of Submicron-Sized Particles by Reduction of Optical Background Signals.

Authors:

Arkesteijn, Ger J A and Lozano-Andrés, Estefanía and Libregts, Sten F W M and Wauben, Marca H M

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2020)

Spectral properties

Correction factor (280 nm)	0.175
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	574
Quantum yield	0.82

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on October 27, 2026

Component A: ReadiUse™ Preactivated PE	1 vial (1 mg)
Component B: Buccutite™ MTA	1 vial (100 μg)
Component C: Spin Desalting Column	Not Included