Name: ReadiUse™ Preactivated PE-iFluor® 660 Tandem
Brand: AAT Bioquest
SKU: 2579
Price: 415 USD
Availability: InStock

ReadiUse™ Preactivated PE-iFluor® 660 Tandem

ReadiUse™ Preactivated PE-iFluor® 660 Tandem is a ready-to-conjugate fluorescent tandem dye for labeling antibodies and other proteins for multicolor and spectral flow cytometry.

Fluorescence Detection: Excitation at 565 nm and emission at 695 nm.
Conjugation Chemistry: Buccutite™ FOL/MTA chemistry enables protein conjugation through amino-group modification.
High Conjugation Yield: Higher yields than conventional SMCC-based chemistry.
Ready-to-Use: Includes preactivated dye and Buccutite™ MTA reagent.
Spectral Compatibility: Emission between Cy5 and Cy5.5 supports spectral flow cytometry.

Our preactivated PE-iFluor® 660 Tandem was premodified with our Buccutite™ FOL (provided). Your antibody (or other proteins) is modified with our Buccutite™ MTA (provided as free sample) to give MTA-modified protein (such as antibody). The MTA-modified protein readily reacts with FOL-modified PE-iFluor® 660 Tandem (provided) to give the desired PE-iFluor® 660 Tandem-antibody conjugate in much higher yield than the SMCC chemistry. In addition our preactivated PE-iFluor® 660 Tandem reacts with MTA-modified biopolymers at much lower concentrations than the SMCC chemistry.

Protocol

SDS

Catalog	Size	Price	Quantity
2579	1 mg	Price

Overview

R-Phycoerythrin (PE) is isolated from red algae. Its primary absorption peak is at 565 nm with secondary peaks at 496 and 545 nm. Our RPE-iFluor® 660 Tandem is an excellent color for multicolor analysis of multiple targets with flow cytometry. It has a unique emission located between the emission Cy5 and that of Cy5.5, providing a new color for spectral flow cytometry. ReadiUse™ Preactivated PE-iFluor® 660 Tandem is an activated PE protein, and can be easily conjugated to antibodies with much higher conjugation yield than the conventional PE.

References

View all 50 references: Citation Explorer

One-Pot Fabrication of Pd Nanoparticles@Covalent-Organic-Framework-Derived Hollow Polyamine Spheres as a Synergistic Catalyst for Tandem Catalysis.

Authors:

Yang, Xinyi and He, Yajun and Li, Liuyi and Shen, Jinni and Huang, Jianhui and Li, Lingyun and Zhuang, Zanyong and Bi, Jinhong and Yu, Yan

Journal:

Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 1864-1870

Analysis of Phospholipids, Lysophospholipids, and Their Linked Fatty Acyl Chains in Yellow Lupin Seeds (Lupinus luteus L.) by Liquid Chromatography and Tandem Mass Spectrometry.

Authors:

Calvano, Cosima Damiana and Bianco, Mariachiara and Ventura, Giovanni and Losito, Ilario and Palmisano, Francesco and Cataldi, Tommaso R I

Journal:

Molecules (Basel, Switzerland) (2020)

Detection of Phytoestrogen Metabolites in Breastfed Infants' Urine and the Corresponding Breast Milk by Liquid Chromatography-Tandem Mass Spectrometry.

Authors:

Min, Jialing and Wang, Zitong and Liang, Chenglin and Li, Wenjie and Shao, Jie and Zhu, Kunrui and Zhou, Lu and Cheng, Jing and Luo, Shihong and Yu, Lehan and Wu, Yudong and Xie, Mingyong and Hu, Xiaojuan

Journal:

Journal of agricultural and food chemistry (2020): 3485-3494

Identification and quantification of phospholipids in strawberry seeds and pulp (Fragaria × ananassa cv San Andreas) by liquid chromatography with electrospray ionization and tandem mass spectrometry.

Authors:

Bianco, Mariachiara and Calvano, Cosima D and Huseynli, Lachin and Ventura, Giovanni and Losito, Ilario and Cataldi, Tommaso R I

Journal:

Journal of mass spectrometry : JMS (2020): e4523

A monolithic nanostructured-perovskite/silicon tandem solar cell: feasibility of light management through geometry and materials selection.

Authors:

Elshorbagy, Mahmoud H and López-Fraguas, Eduardo and Chaudhry, Fateh A and Sánchez-Pena, José Manuel and Vergaz, Ricardo and García-Cámara, Braulio

Journal:

Scientific reports (2020): 2271

Spectral properties

Absorbance (nm)	566
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	695

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on October 15, 2026

A: ReadiUse™ Preactivated PE-iFluor® 660 Tandem	1 vial (1 mg)
B: Buccutite™ MTA	1 vial (100 μg)
C: Spin Desalting Column	Not Included