ReadiUse™ Preactivated PE-iFluor® 700 Tandem

ReadiUse™ Preactivated PE-iFluor® 700 Tandem is a ready-to-conjugate fluorescent tandem dye for labeling antibodies and other proteins for multicolor flow cytometry.

Fluorescence Detection: Excitation at 565 nm and emission at 708 nm.
Conjugation Chemistry: Buccutite™ FOL/MTA chemistry enables protein conjugation through amino-group modification.
High Conjugation Yield: Higher yields than conventional SMCC-based chemistry.
Ready-to-Use: Preactivated dye simplifies antibody conjugation.
Instrument Compatibility: Compatible with 488 nm and 561 nm laser excitation.

Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE/iFlour® 700 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 700 specific B10-A channel.

Protocol

SDS

Catalog	Size	Price	Quantity
2585	1 mg	Price

Overview

PE-Alexa Fluor® 700 is a popular color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at 720 nm. AAT Bioquest offers this preactivated PE-iFluor® 700™ as a superior replacement to the popular PE- Alexa Fluor® 700 tandem. Compared to PE-Alexa Fluor® 700 tandem, PE-iFluor® 700 is brighter with better FRET efficiency. It is used to facilitate the PE-Alexa Fluor® 700 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE-iFluor® 700 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based PE-Alexa Fluor® 700 tandem conjugation chemistry with better flow cytometry performance. In addition, our preactivated PE-iFluor® 700 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.

References

View all 7 references: Citation Explorer

Performance of optoacoustic and fluorescence imaging in detecting deep-seated fluorescent agents.

Authors:

Chen, Zhenyue and Deán-Ben, Xosé Luís and Gottschalk, Sven and Razansky, Daniel

Journal:

Biomedical optics express (2018): 2229-2239

An enzymatically-sensitized sequential and concentric energy transfer relay self-assembled around semiconductor quantum dots.

Authors:

Samanta, Anirban and Walper, Scott A and Susumu, Kimihiro and Dwyer, Chris L and Medintz, Igor L

Journal:

Nanoscale (2015): 7603-14

Multicolor detection of rare tumor cells in blood using a novel flow cytometry-based system.

Authors:

Watanabe, Masaru and Uehara, Yuri and Yamashita, Namiko and Fujimura, Yuu and Nishio, Kaori and Sawada, Takeshi and Takeda, Kazuo and Koizumi, Fumiaki and Koh, Yasuhiro

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2014): 206-13

Noninvasive and quantitative assessment of in vivo fetomaternal interface angiogenesis using RGD-based fluorescence.

Authors:

Keramidas, M and Lavaud, J and Sergent, F and Hoffmann, P and Brouillet, S and Feige, J-J and Coll, J-L and Alfaidy, N

Journal:

BioMed research international (2014): 309082

Intraoperative near-infrared image-guided surgery for peritoneal carcinomatosis in a preclinical experimental model.

Authors:

Keramidas, M and Josserand, V and Righini, C A and Wenk, C and Faure, C and Coll, J L

Journal:

The British journal of surgery (2010): 737-43

Spectral properties

Absorbance (nm)	566
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	708

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on October 14, 2026

Component A: ReadiUse™ Preactivated PE-iFluor® 700 Tandem	1 vial (1 mg)
Component B: Buccutite™ MTA	1 vial (100 μg)
Component C: Spin Desalting Column	Not Included