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ReadiUse™ Preactivated PE-iFluor® 700 Tandem
PE-Alexa Fluor® 700 is a popular color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at 720 nm. AAT Bioquest offers this preactivated PE-iFluor 700™ as a superior replacement to the popular PE- Alexa Fluor® 700 tandem. Compared to PE-Alexa Fluor® 700 tandem, PE-iFluor 700 is brighter with better FRET efficiency. It is used to facilitate the PE-Alexa Fluor® 700 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE-iFluor 700 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based PE-Alexa Fluor® 700 tandem conjugation chemistry with better flow cytometry performance. In addition, our preactivated PE-iFluor 700 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE/iFlour® 700 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 700 specific B10-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE/iFlour® 700 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 700 specific B10-A channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
25851 mg
Price
 
Physical properties
Molecular weightN/A
SolventWater
Spectral properties
Absorbance (nm)566
Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)565
Emission (nm)708
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 13, 2025