Name: ReadiUse™ Preactivated Streptavidin Azide
Brand: AAT Bioquest
SKU: 16883
Price: 412 USD
Availability: InStock

ReadiUse™ Preactivated Streptavidin Azide

Click Chemistry: Azide groups react with alkyne-containing compounds.
Broad Reactivity: Compatible with conventional alkynes, DBCO, and FastClick™ alkynes.
High Biotin Affinity: Binds up to 4 moles of biotin per streptavidin.
Low Background: Reduced nonspecific binding compared to avidin.
Applications: Suitable for bioconjugation and biotin-based detection assays.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
16883	1 mg	Price

Overview

ReadiUse™ Preactivated Streptavidin Azide is an excellent alkyne-reactive building block that can be used for developing bioconjugates via the well-known click chemistry. It readily reacts with conventional alkynes, DBCO and the FastClick™ alkynes. Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces avidinii. Streptavidin binds 4 moles of biotin per mole of protein with a high affinity virtually unmatched in nature. Streptavidin lacks carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality where most useful biological interactions occur (pI of 5-6 vs 10 for avidin). As a result, streptavidin frequently exhibits lower levels of non-specific binding than does avidin when the proteins are used in applications relying upon the formation of avidin/biotin complexes. Streptavidin finds utility in a variety of biological systems because antibody molecules are easily modified by the covalent attachment of derivatives of biotin with little or no loss in the ability of the antibody molecules to bind their antigens. These biotinylated antibodies may be detected by their interaction with conjugates of streptavidin and the reporter enzymes. The same preparation of conjugated streptavidin reporter enzyme may be used with any number of different biotinylated antibodies making this system a highly flexible one. The reporter molecule may be bound to streptavidin covalently, or biotinylated and attached to streptavidin via the streptavidin-biotin interaction.

References

View all 50 references: Citation Explorer

Chemiluminescent plate assay of microRNA-155 coupled with catalytic hairpin assembly with oligonucleotide release (CHAOR).

Authors:

Bodulev, Oleg L and Zakharov, Maxim D and Sakharov, Ivan Yu

Journal:

Talanta (2024): 125525

Fluorescence Multi-Detection Device Using a Lensless Matrix Addressable microLED Array.

Authors:

Moro, Victor and Canals, Joan and Moreno, Sergio and Higgins-Wood, Steffen and Alonso, Oscar and Waag, Andreas and Prades, J Daniel and Dieguez, Angel

Journal:

Biosensors (2024)

A sensitive fluoroimmunoassay for quantitative detection of imidacloprid based on quantum dot-streptavidin conjugate.

Authors:

Zhang, Binbin and Li, Shi and Dong, Fengshou and Xu, Jun and Wu, Xiaohu and Zheng, Yongquan and Pan, Xinglu

Journal:

Talanta (2024): 126128

Biotinylated Fluorescent Polymeric Nanoparticles for Enhanced Immunostaining.

Authors:

Yudhistira, Tesla and Da Silva, Elisabete Cruz and Combes, Antoine and Lehmann, Maxime and Reisch, Andreas and Klymchenko, Andrey S

Journal:

Small methods (2023): e2201452

Novel Pneumococcal Protein-Polysaccharide Conjugate Vaccine Based on Biotin-Streptavidin.

Authors:

Guo, Mengze and Guo, Xiaonan and Zhang, Chenxing and Zhu, Shidong and Zhang, Yue and Gu, Tiejun and Kong, Wei and Wu, Yongge

Journal:

Infection and immunity (2022): e0035221

Physical properties

Solvent

Water

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 26, 2026