Name: ReadiUse™ Preactivated Streptavidin Maleimide
Brand: AAT Bioquest
SKU: 16887
Price: 217 USD
Availability: InStock

ReadiUse™ Preactivated Streptavidin Maleimide

ReadiUse™ Preactivated Streptavidin Maleimide is a thiol-reactive streptavidin reagent for conjugating streptavidin to thiol-containing biomolecules.

Maleimide Chemistry: Enables covalent conjugation to thiol groups.
Mild Reaction Conditions: Supports conjugation under mild conditions.
High Biotin Affinity: Binds up to 4 moles of biotin per streptavidin.
Low Background: Lower nonspecific binding than avidin.
Applications: Suitable for bioconjugation and biotin-based detection assays.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
16887	1 mg	Price

Overview

ReadiUse™ Preactivated Streptavidin Maleimide is an excellent thiol-reactive building block that can be readily conjugated to thiol-containing biomolecules such as antibodies, peptides and thiol-modified oligos. It reacts with these thiol compounds under extremely mild conditions. Streptavidin binds 4 moles of biotin per mole of protein with a high affinity virtually unmatched in nature. Streptavidin lacks carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality where most useful biological interactions occur (pI of 5-6 vs 10 for avidin). As a result, streptavidin frequently exhibits lower levels of non-specific binding than does avidin when the proteins are used in applications relying upon the formation of avidin/biotin complexes. Streptavidin finds utility in a variety of biological systems because antibody molecules are easily modified by the covalent attachment of derivatives of biotin with little or no loss in the ability of the antibody molecules to bind their antigens. These biotinylated antibodies may be detected by their interaction with conjugates of streptavidin and the reporter enzymes. The same preparation of conjugated streptavidin reporter enzyme may be used with any number of different biotinylated antibodies making this system a highly flexible one. The reporter molecule may be bound to streptavidin covalently, or biotinylated and attached to streptavidin via the streptavidin-biotin interaction.

References

View all 50 references: Citation Explorer

Modified streptavidin-biotin based lateral flow test strip for rapid detection of SARS-CoV-2 S1 antigen in saliva samples.

Authors:

Kamel, Manal and Atta, Shimaa and Maher, Sara and Abd Elaziz, Hesham and Demerdash, Zeinab

Journal:

Scientific reports (2024): 7319

Streptavidin-biotin system-mediated immobilization of a bivalent nanobody onto magnetosomes for separation and analysis of 3-phenoxybenzoic acid in urine.

Authors:

Tang, Fang and Wang, Yating and Wang, Di and Yang, Yayun and Chang, Jiashu and Sun, Huabo and Gu, Shaopeng and He, Jinxin

Journal:

Analytical methods : advancing methods and applications (2024): 1546-1553

Development and validation of streptavidin-biotin-based double antibody sandwich ELISA for ricin diagnosis.

Authors:

Dixit, Shivani and Parashar, Jagrati and Dhaked, Ram Kumar and Kumar, Abdhesh and Saxena, Nandita

Journal:

International immunopharmacology (2024): 111986

A Lateral Flow Assay Based on Streptavidin-biotin Amplification System with Recombinase Polymerase Amplification for Rapid and Quantitative Detection of Salmonella enteritidis.

Authors:

Feng, Fan and Fu, Qiang and Cao, Fengrong and Yuan, Yun and Kong, Ruixue and Ji, Dandan and Liu, Haiyun

Journal:

Chembiochem : a European journal of chemical biology (2024): e202300575

A streptavidin-biotin system combined with magnetic actuators for remote neuronal guidance.

Authors:

Levenberg, Dafna Rivka and Varon, Eli and Indech, Ganit and Ben Uliel, Tal and Geri, Lidor and Sharoni, Amos and Shefi, Orit

Journal:

Journal of biological engineering (2023): 40

Physical properties

Molecular weight	~55 kDa
Solvent	Water

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 25, 2026