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ReadiUse™ Preactivated Streptavidin NHS Ester

ReadiUse™ Preactivated Streptavidin NHS Ester is an excellent amino-reactive building block that can be readily conjugated to antibodies, peptides and amino-modified oligos. It reacts with these amino compounds under extremely mild conditions. Streptavidin binds 4 moles of biotin per mole of protein with a high affinity virtually unmatched in nature. Streptavidin lacks carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality where most useful biological interactions occur (pI of 5-6 vs 10 for avidin). As a result, streptavidin frequently exhibits lower levels of non-specific binding than does avidin when the proteins are used in applications relying upon the formation of avidin/biotin complexes. Streptavidin finds utility in a variety of biological systems because antibody molecules are easily modified by the covalent attachment of derivatives of biotin with little or no loss in the ability of the antibody molecules to bind their antigens. These biotinylated antibodies may be detected by their interaction with conjugates of streptavidin and the reporter enzymes. The same preparation of conjugated streptavidin reporter enzyme may be used with any number of different biotinylated antibodies making this system a highly flexible one. The reporter molecule may be bound to streptavidin covalently, or biotinylated and attached to streptavidin via the streptavidin-biotin interaction.

References

View all 50 references: Citation Explorer
Biotin as a structural component in the detection of small model antigens in BLA-S-ELISA.
Authors: Simonović, Mladen and Ostojić, Sanja and Micić, Darko and Djurdjić, Petar and Mix, Thorsten and Kuzmanović, Čeda and Jelovac, Drago
Journal: Natural product research (2024): 1-5
Streptavidin-biotin system-mediated immobilization of a bivalent nanobody onto magnetosomes for separation and analysis of 3-phenoxybenzoic acid in urine.
Authors: Tang, Fang and Wang, Yating and Wang, Di and Yang, Yayun and Chang, Jiashu and Sun, Huabo and Gu, Shaopeng and He, Jinxin
Journal: Analytical methods : advancing methods and applications (2024): 1546-1553
Development and validation of streptavidin-biotin-based double antibody sandwich ELISA for ricin diagnosis.
Authors: Dixit, Shivani and Parashar, Jagrati and Dhaked, Ram Kumar and Kumar, Abdhesh and Saxena, Nandita
Journal: International immunopharmacology (2024): 111986
Evaluation of a biotin-based surrogate virus neutralization test for detecting postvaccination antibodies against SARS-CoV-2 variants in sera.
Authors: Ahn, Min-Ju and Kang, Jung-Ah and Hong, Su Min and Lee, Kyu-Sun and Kim, Dong Ho and Song, Daesub and Jeong, Dae Gwin
Journal: Biochemical and biophysical research communications (2023): 8-18
Effects of High-Biotin Sample Interference on Antibody Concentrations in Sandwich Immunoassays.
Authors: Balieiro Neto, Geraldo and Engracia Filho, Jair Rodini and Budino, Fabio Enrique Lemos and Freitas, Acyr Wanderley de Paula and Soares, Weber Vilas Boas
Journal: Vaccines (2023)
Page updated on April 25, 2025

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Catalog Number16888
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Physical properties

Solvent

Water

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure