Name: ReadiUse™ Rapid Luminometric ATP Assay Kit
Brand: AAT Bioquest
SKU: 21601
Availability: InStock

ReadiUse™ Rapid Luminometric ATP Assay Kit

ReadiUse™ Rapid Luminometric ATP Assay Kit is a luminometric assay kit for quantifying ATP and monitoring cell proliferation and cytotoxicity in mammalian cells.

Detection Principle: Uses firefly luciferase to generate ATP-dependent luminescence.
Sensitivity: Detects as few as 50 cells/well.
Signal Stability: Luminescent signal has a half-life greater than 2 hours.
Workflow: Homogeneous assay with a 10–20 minute room-temperature incubation.
Formulation: DTT-free and supplied in a ready-to-use format.

Cell number correlates with the luminescent signal. Different Jurkat cell number (2-fold dilution) was measured using the ReadiUse™ Rapid Luminometric ATP Assay Kit in a 96-well white plate using a ClarioStar plate reader (BMG Labtech). The kit can detect as low as 50 cells. There is a linear relationship (r2 >0.99) between the luminescent signal and cell number after 15 minutes or 2 hours incubation. The half-life of luminescent signal is more than 2 hours.

Protocol

COA

Catalog	Size	Price
21601	100 Tests	Price
21602	1000 Tests	Price
21603	10000 Tests	Price

Overview

Adenosine triphosphate (ATP) plays a fundamental role in cellular energetics, metabolic regulation and cellular signaling. The quantitation of ATP can be used for a variety of biological applications. Because ATP is the energy source for almost all living organisms that rapidly degrades in the absence of viable organisms, its existence can be used to identify the presence of viable organisms. The measurement of ATP has been used for cell cytotoxicity, detection of bacteria on surfaces, quantification of bacteria in water, somatic cells in culture and food quality. The use of firefly bioluminescence to measure ATP was first proposed by McElroy when he discovered that ATP was essential for light production. Firefly luciferase is a monomeric 61 kD enzyme that catalyzes a two-step oxidation of luciferin, which yields light at 560 nm. The first step involves the activation of the protein by ATP to produce a reactive mixed anhydride intermediate. In the second step, the active intermediate reacts with oxygen to create a transient dioxetane, which quickly breaks down to the oxidized product oxyluciferin and carbon dioxide along with a burst of light. When ATP is the limiting component, the intensity of light is proportional to the concentration of ATP. Thus the measurement of the light intensity can be used for quantifying ATP using a luminometer. AAT Bioquest's ReadiUse™ Rapid Luminometric ATP Assay Kit (DTT free) comes with all the essential components in a ready-to-use format. It provides a fast, simple and homogeneous luminescence assay for monitoring cell proliferation and cytotoxicity in mammalian cells. This assay is based on the detection of ATP using firefly luciferase to catalyze the release of light by ATP and luciferin. It can be performed in a convenient 96-well or 384-well microtiter-plate format on a chemiluminescent microplate reader. The assay is extremely sensitive and can detect 50 cells/well. It has stable luminescent signal with half-life more than 2 hours. This ReadiUse™ Rapid Luminometric ATP Assay Kit does not use DTT, which eliminating the unpleasant odor.

Citations

View all 16 citations: Citation Explorer

Responses of RTgill-W1 cells to cyanobacterial metabolites microcystin-LR, anabaenopeptin-A, cylindrospermopsin, their binary and ternary mixtures

Authors:

Bownik, Adam and Pawlik-Skowro{\'n}ska, Barbara

Journal:

Toxicon (2024): 108059

Palmitic Acid Exerts Anti-Tumorigenic Activities by Modulating Cellular Stress and Lipid Droplet Formation in Endometrial Cancer

Authors:

Zhao, Ziyi and Wang, Jiandong and Kong, Weimin and Newton, Meredith A and Burkett, Wesley C and Sun, Wenchuan and Buckingham, Lindsey and O’Donnell, Jillian and Suo, Hongyan and Deng, Boer and others,

Journal:

Biomolecules (2024): 601

Combining Cisplatin with Pinellia pedatisecta Schott Lipid-Soluble Extract Induces Tumor Immunogenic Cell Death in Cervical Cancer

Authors:

Wang, Congwen and Zhang, Mingxing and Peng, Jing and Zhang, Meng and Lu, Chong and Qi, Xingling and Luo, Qingyan and Wang, Yumeng and Li, Guiling

Journal:

Phytomedicine (2024): 155504

Homogeneous luminescent quantitation of cellular guanosine and adenosine triphosphates (GTP and ATP) using QT-LucGTP\&ATP assay

Authors:

Kopra, Kari and Mahran, Randa and Yli-Hollo, Titta and Tabata, Sho and Vuorinen, Emmiliisa and Fujii, Yuki and Vuorinen, Iida and Ogawa-Iio, Aki and Hirayama, Akiyoshi and Soga, Tomoyoshi and others,

Journal:

Analytical and Bioanalytical Chemistry (2023): 1--12

Guidelines for cell viability assays

Authors:

Kamiloglu, Senem and Sari, Gulce and Ozdal, Tugba and Capanoglu, Esra

Journal:

Food Frontiers (2020): 332--349

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Luminescence microplate reader
Recommended plate	Solid white

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Page updated on October 23, 2026