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ReadiUse™ Rapid Luminometric ATP Assay Kit
Adenosine triphosphate (ATP) plays a fundamental role in cellular energetics, metabolic regulation and cellular signaling. The quantitation of ATP can be used for a variety of biological applications. Because ATP is the energy source for almost all living organisms that rapidly degrades in the absence of viable organisms, its existence can be used to identify the presence of viable organisms. The measurement of ATP has been used for cell cytotoxicity, detection of bacteria on surfaces, quantification of bacteria in water, somatic cells in culture and food quality. The use of firefly bioluminescence to measure ATP was first proposed by McElroy when he discovered that ATP was essential for light production. Firefly luciferase is a monomeric 61 kD enzyme that catalyzes a two-step oxidation of luciferin, which yields light at 560 nm. The first step involves the activation of the protein by ATP to produce a reactive mixed anhydride intermediate. In the second step, the active intermediate reacts with oxygen to create a transient dioxetane, which quickly breaks down to the oxidized product oxyluciferin and carbon dioxide along with a burst of light. When ATP is the limiting component, the intensity of light is proportional to the concentration of ATP. Thus the measurement of the light intensity can be used for quantifying ATP using a luminometer. AAT Bioquest's ReadiUse™ Rapid Luminometric ATP Assay Kit (DTT free) comes with all the essential components in a ready-to-use format. It provides a fast, simple and homogeneous luminescence assay for monitoring cell proliferation and cytotoxicity in mammalian cells. This assay is based on the detection of ATP using firefly luciferase to catalyze the release of light by ATP and luciferin. It can be performed in a convenient 96-well or 384-well microtiter-plate format on a chemiluminescent microplate reader. The assay is extremely sensitive and can detect 50 cells/well. It has stable luminescent signal with half-life more than 2 hours. This ReadiUse™ Rapid Luminometric ATP Assay Kit does not use DTT, which eliminating the unpleasant odor.
Cell number correlates with the luminescent signal. Different Jurkat cell number (2-fold dilution) was measured using the ReadiUse™ Rapid Luminometric ATP Assay Kit in a 96-well white plate using a ClarioStar plate reader (BMG Labtech). The kit can detect as low as 50 cells. There is a linear relationship (r2 >0.99) between the luminescent signal and cell number after 15 minutes or 2 hours incubation. The half-life of luminescent signal is more than 2 hours.
Cell number correlates with the luminescent signal. Different Jurkat cell number (2-fold dilution) was measured using the ReadiUse™ Rapid Luminometric ATP Assay Kit in a 96-well white plate using a ClarioStar plate reader (BMG Labtech). The kit can detect as low as 50 cells. There is a linear relationship (r2 >0.99) between the luminescent signal and cell number after 15 minutes or 2 hours incubation. The half-life of luminescent signal is more than 2 hours.
protocol
Protocol
COA
CatalogSize
Price
Quantity
21601100 Tests
Price
216021000 Tests
Price
2160310000 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Luminescence microplate reader
Recommended plateSolid white
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Page updated on October 8, 2025