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ReadiUse™ WST-8
50 mM aqueous solution
WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt) is a water-soluble tetrazolium salt used for assessing cell metabolic activity. At neutral pH and in the presence of the intermediate electron acceptor, 1-methoxy phenazine methosulfate, NADPH-dependent cellular oxidoreductases, acting via plasma membrane electron transport, reduce the colorless cell-impermeant WST-8 outside the cell to a water-soluble orange formazan dye with absorbance max at 460 nm. WST-8 is very stable, and tolerates a long time incubation (such as 24 to 48 hours) due to its little cytotoxicity. WST-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salts such as MTT, XTT or MTS. It is typically used as a cell viability indicator in cell proliferation assays.
Chemical structure for ReadiUse™ WST-8 *50 mM aqueous solution*
Chemical structure for ReadiUse™ WST-8 *50 mM aqueous solution*
CatalogSize
Price
Quantity
1570525 mg
Price
15706100 mg
Price
157071 g
Price
 
Physical properties

Molecular weight600.5
SolventWater
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
CAS193149-74-5
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Page updated on September 24, 2025