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ReadiUse™ WST-8 *50 mM aqueous solution*

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Physical properties
Molecular weight600.5
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Molecular weight
WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt) is a water-soluble tetrazolium salt used for assessing cell metabolic activity. At neutral pH and in the presence of the intermediate electron acceptor, 1-methoxy phenazine methosulfate, NADPH-dependent cellular oxidoreductases, acting via plasma membrane electron transport, reduce the colorless cell-impermeant WST-8 outside the cell to a water-soluble orange formazan dye with absorbance max at 460 nm. WST-8 is very stable, and tolerates a long time incubation (such as 24 to 48 hours) due to its little cytotoxicity. WST-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salts such as MTT, XTT or MTS. It is typically used as a cell viability indicator in cell proliferation assays.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of ReadiUse™ WST-8 *50 mM aqueous solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM166.528 µL832.639 µL1.665 mL8.326 mL16.653 mL
5 mM33.306 µL166.528 µL333.056 µL1.665 mL3.331 mL
10 mM16.653 µL83.264 µL166.528 µL832.639 µL1.665 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles



View all 5 citations: Citation Explorer
Cytotoxicity effect and transcriptome analysis of PCV3-infected cells revealed potential viral pathogenic mechanisms
Authors: Chang, Chia-Chun and Wu, Ching-Ying and Wu, Chi-Ming and Wu, Ching-Wei and Wang, Yi-Chen and Lin, Guang-Jan and Chien, Maw-Sheng and Huang, Chienjin
Journal: Microbial Pathogenesis (2024): 106715
Exploring the surface epitope and nuclear localization analysis of porcine circovirus type 3 capsid protein
Authors: Chang, Chia-Chun and Wu, Ching-Ying and Ciou, Jhao-Guan and Wu, Ching-Wei and Wang, Yi-Chen and Chang, Hui-Wen and Chien, Maw-Sheng and Huang, Chienjin
Journal: AMB Express (2023): 1--11
miR-218 inhibits the proliferation of human glioma cells through downregulation of Yin Yang 1
Authors: Gao, Yong and Sun, Laisheng and Wu, Zicheng and Xuan, Chengmin and Zhang, Junxia and You, Yongping and Chen, Xincheng
Journal: Molecular Medicine Reports (2018): 1926--1932
MiR-218 suppresses osteosarcoma proliferation by down-regulating BMI1
Authors: Xuan, Chengmin and Jin, Mingwei and Gao, Yong and Xu, Shumei and Wang, Lei and Wang, Yuan and Han, Rui and An, Qi
miR-218 inhibits the proliferation of human glioma cells through downregulation of Yin Yang 1
Authors: Gao, Yong and Sun, Laisheng and Wu, Zicheng and Xuan, Chengmin and Zhang, Junxia and You, Yongping and Chen, Xincheng
Journal: Molecular Medicine Reports (2017)


View all 5 references: Citation Explorer
A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line
Authors: Tiwari, K.; Wavdhane, M.; Haque, S.; Govender, T.; Kruger, H. G.; Mishra, M. K.; Ch and ra, R.; Tiwari, D.
Journal: Pharm Biol (2015): 849-54
Comparison of the WST-8 colorimetric method and the CLSI broth microdilution method for susceptibility testing against drug-resistant bacteria
Authors: Tsukatani, T.; Suenaga, H.; Shiga, M.; Noguchi, K.; Ishiyama, M.; Ezoe, T.; Matsumoto, K.
Journal: J Microbiol Methods (2012): 160-6
WST-8 analysis of cell viability during osteogenesis of human mesenchymal stem cells
Authors: Stoddart, M. J.
Journal: Methods Mol Biol (2011): 21-5
monosodium salt (WST-8) rapid colorimetric assays for antimicrobial susceptibility testing of staphylococci and ESBL-producing clinical isolates
Authors: Brady, A. J.; Kearney, P.; Tunney, M. M., Comparative evaluation of 2,3-bis [2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) and 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium
Journal: J Microbiol Methods (2007): 305-11
Reactivity of blood samples spotted onto filter papers in the WST-8 method for screening of G6PD deficiency
Authors: Arai, M.; Kosuge, K.; Kawamoto, F.; Matsuoka, H.
Journal: Acta Med Okayama (2006): 127-34