CFSE | AAT Bioquest

ReadiUse™ CFSE

5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester; CAS 150347-59-4

It is widely recognized that fluorescent labeling of cells is an effective means to determine total cell numbers or how many viable cells exist in a sample. Flow cytometry combined with fluorescent staining is a powerful tool to analyze heterogeneous cell populations. Fluorescein diacetate (FDA) and its derivatives are non-fluorescent molecules that diffuse into cells and are hydrolyzed by intracellular non-specific esterases to give fluorescent products. The fluorescent products can be accumulated only in those cells that have intact cell membranes; therefore, dead cells with leaky membranes are not stained. The precise kinetics of membrane transport and intracellular hydrolysis of FDA and its analogs (such as CDCFDA) are related to cellular functions, thus FDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The fluorescence intensity of labeled cells by FDA dyes varies considerably among cell lines and strains, probably because of differences in intracellular esterase activity. CFSE is an amine-reactive FDA derivative that is widely used for monitoring cell proliferation with a flow cytometer. AAT Bioquest offers this convenient package size for flow cytometric use. Each vial of CFSE may be reconstituted to a stock concentration of 10 mM with 90 uL of anhydrous DMSO. The DMSO stock solution should be protected from light and stored at -20°C with dessicant, avoiding freeze-thawing.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22028	5x500 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiUse™ CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *CAS 150347-59-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	179.385 µL	896.925 µL	1.794 mL	8.969 mL	17.939 mL
5 mM	35.877 µL	179.385 µL	358.77 µL	1.794 mL	3.588 mL
10 mM	17.939 µL	89.693 µL	179.385 µL	896.925 µL	1.794 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	557.46 g/mol	=	mL	x	mM	=	mmol

Citations

View all 12 citations: Citation Explorer

Microalgal cell division tracking using CFSE

Authors:

Pozzobon, Victor and Lagirarde, Jules and Arnoudts, Clarisse and Levasseur, Wendie

Journal:

Algal Research (2024): 103501

Hypoxia-activated ADCC-enhanced humanized anti-CD147 antibody for liver cancer imaging and targeted therapy with improved selectivity

Authors:

Qi, Fang-Zheng and Su, Hui-Shan and Wang, Bo and Qian, Luo-Meng and Wang, Yang and Wang, Chen-Hui and Hou, Ya-Xin and Chen, Ping and Zhang, Qing and Li, Dong-Mei and others,

Journal:

MedComm (2024)

Restoration of miR-299-3p promotes macrophage phagocytosis and suppresses malignant phenotypes in breast cancer carcinogenesis via dual-targeting CD47 and ABCE1

Authors:

Tong, Shoufang and Zhu, Yingli and Leng, Yeqing and Wu, Yunling and Xiao, Xingxing and Zhao, Wenfeng and Tan, Shuhua

Journal:

International Immunopharmacology (2024): 111708

Proof-of-principle studies on a strategy to enhance nucleotide imbalance specifically in cancer cells

Authors:

Alkasalias, Twana and Zhang, Juan and Madapura, Harsha and Dalaroun, Basile and Reina, Oscar Bedoya and Lewensohn, Rolf and Viktorsson, Kristina and Salihi, Abbas and Darekar, Suhas and La{\'\i}n, Sonia

Journal:

Cell death discovery (2022): 1--14

Proof-of-principle studies on a strategy to enhance nucleotide imbalance specifically in cancer cells

Authors:

Journal:

Cell Death Discovery (2022): 1--14

References

View all 68 references: Citation Explorer

Novel method for cell debris removal in the flow cytometric cell cycle analysis using carboxy-fluorescein diacetate succinimidyl ester

Authors:

Terho P, Lassila O.

Journal:

Cytometry A (2006): 552

Modification of the fluorescein diacetate assay for screening of antifungal agents against Candida albicans: comparison with the NCCLS methods

Authors:

Brouwer N, Kohen J, Jamie J, Vemulpad S.

Journal:

J Microbiol Methods (2006): 234

Optimisation of the fluorescein diacetate antibacterial assay

Authors:

Wan, undefined and y S, Brouwer N, Liu Q, Mahon A, Cork S, Karuso P, Vemulpad S, Jamie J.

Journal:

J Microbiol Methods (2005): 21

A three-dimensional flow control concept for single-cell experiments on a microchip. 2. Fluorescein diacetate metabolism and calcium mobilization in a single yeast cell as stimulated by glucose and pH changes

Authors:

Peng XY, Li PC.

Journal:

Anal Chem (2004): 5282

Effect of immunosuppressants on T-cell subsets observed in vivo using carboxy-fluorescein diacetate succinimidyl ester labeling

Authors:

Hu H, Dong Y, Feng P, Fechner J, Hamawy M, Knechtle SJ.

Journal:

Transplantation (2003): 1075

Physical properties

Molecular weight	557.46
Solvent	DMSO

Spectral properties

Excitation (nm)	498
Emission (nm)	517

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	150347-59-4

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Page updated on September 30, 2024