Name: ReadiUse™ MTS Reagent *5 mM aqueous solution*
Brand: AAT Bioquest
SKU: 15710
Availability: InStock

ReadiUse™ MTS Reagent

5 mM aqueous solution

ReadiUse™ MTS Reagent is a water-soluble tetrazolium reagent for quantifying cell viability and proliferation through colorimetric measurement of cellular metabolic activity.

Colorimetric Detection: Generates a soluble formazan product measurable at 490 nm.
Ready-to-Use: Supplied as a 5 mM aqueous solution.
Metabolic Activity: NAD(P)H-dependent enzymes reduce MTS to formazan.
Microplate Compatible: Supports absorbance measurement in clear-bottom 96-well plates.
Applications: Suitable for cell viability, proliferation, and cytotoxicity assays.

Cell numbers were quantified using the ReadiUse™ MTS Reagent (Cat# 15710). HeLa cells, ranging from 781 to 50,000 cells per well in 100 µL, were seeded into a clear-bottom 96-well plate. After 4 hours of incubation with the MTS reagent, absorbance at 490 nm was measured using a ClarioStar absorbance microplate reader.

Protocol

COA

Catalog	Size	Price	Quantity
15710	5 mL	Price
15711	50 mL	Price

Overview

MTS reagent is one of the most common water-soluble tetrazolium reagents that are widely used to quantify cell viability by absorption spectrophotometry. A variety of tetrazolium inner salts are used for determining cell viability in proliferation assays by monitoring their color changes. Metabolically active cells reduce colorless tetrazolium inner salts into the colored formazan products that might be either soluble or insoluble in cell culture media depending on the structures of tetrazolium reagents. The conversion of tetrazolium inner salts to formazans is thought to be carried out by NAD(P)H-dependent dehydrogenase enzymes.

References

View all 39 references: Citation Explorer

The edge effect: A global problem. The trouble with culturing cells in 96-well plates.

Authors:

Mansoury, Morva and Hamed, Maya and Karmustaji, Rashid and Al Hannan, Fatima and Safrany, Stephen T

Journal:

Biochemistry and biophysics reports (2021): 100987

Profiling the Neoplasm Microenvironment of Silica Nanomaterial-Derived Scaffolds of Single, 2-, and 3-Composite Systems.

Authors:

Akpe, Victor and Murhekar, Shweta and Kim, Tak H and Brown, Christopher L and Cock, Ian E

Journal:

Assay and drug development technologies (2021): 191-203

A Modified MTS Proliferation Assay for Suspended Cells to Avoid the Interference by Hydralazine and β-Mercaptoethanol.

Authors:

Wang, Yutang and Nguyen, Dinh Tam and Yang, Guang and Anesi, Jack and Kelly, Jason and Chai, Zhonglin and Ahmady, Fahima and Charchar, Fadi and Golledge, Jonathan

Journal:

Assay and drug development technologies (2021): 184-190

Synthesis, structural characterization, and prospects for new cobalt (II) complexes with thiocarbamoyl-pyrazoline ligands as promising antifungal agents.

Authors:

Dias, Bianca Boni and da Silva Dantas, Fabiana Gomes and Galvão, Fernanda and Cupozak-Pinheiro, Wellinton Jhon and Wender, Heberton and Pizzuti, Lucas and Rosa, Persiely Pires and Tenório, Kátia Veronica and Gatto, Claudia Cristina and Negri, Melyssa and Casagrande, Gleison Antônio and de Oliveira, Kelly Mari Pires

Journal:

Journal of inorganic biochemistry (2020): 111277

The Predictive Value of Estrogen Receptor 1 on Adjuvant Chemotherapy in Locally Advanced Colorectal Cancer: A Retrospective Analysis With Independent Validation and Its Potential Mechanism.

Authors:

Ye, Shu-Biao and Cheng, Yi-Kan and Deng, Ru and Deng, Yanhong and Li, Peisi and Zhang, Lin and Lan, Ping

Journal:

Frontiers in oncology (2020): 214

Physical properties

Molecular weight	487.51
Solvent	Water

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	138169-43-4

Instrument settings

Absorbance microplate reader
Absorbance	490 nm
Recommended plate	Clear bottom

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Page updated on October 15, 2026