AAT Bioquest

ReadiUse™ MTS Reagent *5 mM aqueous solution*

Product Image
Product Image
Gallery Image 1
Ordering information
Catalog Number
Unit Size
Add to cart
Additional ordering information
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight487.51
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Related products
ReadiUse™ Preactivated APC-iFluor® 700 Tandem
ReadiUse™ Preactivated APC-iFluor® 750 Tandem
ReadiUse™ Preactivated PE-iFluor® 647 Tandem
ReadiUse™ Preactivated PE-iFluor® 750 Tandem
ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
ReadiUse™ hydrogen peroxide solution *50 mM calibrated and stabilized solution*
ReadiUse™ Preactivated HRP NHS ester
ReadiUse™ NADPH Regenerating Kit
ReadiUse™ NADP Regenerating Kit
ReadiUse™ TCA Deproteinization Sample Preparation Kit
ReadiUse™ microscope mounting solution
ReadiUse™ 4% formaldehyde fixation solution
ReadiUse™ mammalian cell lysis buffer *5X*
ReadiUse™ probenecid, sodium salt *Water-soluble*
ReadiUse™ probenecid *25 mM stabilized aqueous solution*
ReadiUse™ CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *CAS 150347-59-4*
ReadiUse™ Preactivated PE
ReadiUse™ Preactivated APC
ReadiUse™ Preactivated PE-Cy5 Tandem
ReadiUse™ Preactivated PE-Cy5.5 Tandem
ReadiUse™ Preactivated PE-Cy7 Tandem
ReadiUse™ Preactivated PE-Texas Red Tandem
ReadiUse™ Preactivated APC-Cy5.5 Tandem
ReadiUse™ Preactivated APC-Cy7 Tandem
ReadiUse™ TCEP removal solution
ReadiUse™ WST-8 *50 mM aqueous solution*
ReadiUse™ Rapid Luminometric ATP Assay Kit
ReadiUse™ PE [R-Phycoerythrin] *Ammonium Sulfate-Free*
ReadiUse™ CL-APC [Cross linked-Allophycocyanin] *Ammonium Sulfate-Free*
ReadiUse™ Preactivated PE-iFluor® 594 Tandem
ReadiUse™ Cell Detaching Buffer
ReadiUse™ DRAQ5 Staining Solution *5 mM in Water*
ReadiUse™ Staurosporine * 1 mM DMSO stock solution*
ReadiUse™ Bio-Gel P-6 spin column
ReadiUse™ bacterial cell lysis buffer *5X*
ReadiUse™ 10% Triton X-100 *Hydrogen Peroxide-and Carbonyl-Free*
ReadiUse™ Preactivated PE-iFluor® 700 Tandem
ReadiUse™ dNTP Mix *10 mM*
ReadiUse™ Viral RNA Lysis Buffer
ReadiUse™ Tyramide (TSA)/Styramide (PSA) Optimized Reaction Buffer
ReadiUse™ Preactivated PerCP
ReadiUse™ Preactivated PerCP-Cy5.5 Tandem
ReadiUse™ Preactivated PE-iFluor® 660 Tandem
ReadiUse™ 10KD Spin Filter
ReadiUse™ Preactivated APC-iFluor® 800 Tandem
ReadiUse™ 1 Kb Plus DNA Ladder
ReadiUse™ GeneRuler 1 kb DNA Ladder
ReadiUse™ 100 bp DNA Ladder
ReadiUse™ ddNTP Terminator Mix *10 mM*
ReadiUse™ dNTP Mix Set *10 mM PCR Grade*
ReadiUse™ Preactivated HRP maleimide
ReadiUse™ Stayright™ Purple *HRP Chromogen Premixed with Hydrogen Peroxide*
ReadiUse™ Disposable PD-10 Desalting Column
ReadiUse™ Preactivated APC Maleimide [Activated Allophycocyanin]
ReadiUse™ Preactivated PE Maleimide [Activated R-Phycoerythrin]
ReadiUse™ 6-Color Human TBNK Antibody Kit *Dry Reagent Format*
ReadiUse™ Preactivated PE-Texas Red Maleimide
ReadiUse™ Preactivated PE-Cy5 Maleimide
ReadiUse™ Preactivated PE-Cy5.5 Maleimide
ReadiUse™ Preactivated PE-Cy7 Maleimide
ReadiUse™ Preactivated APC-AF700 Maleimide
ReadiUse™ Preactivated APC-Cy5.5 Maleimide
ReadiUse™ Preactivated APC-Cy7 Maleimide
ReadiUse™ Preactivated PerCP Maleimide
ReadiUse™ Preactivated PerCP-Cy5.5 Maleimide
ReadiUse™ Preactivated PerCP-Cy7 Maleimide
ReadiUse™ PCR Reaction Buffer
ReadiUse™ Universal Kinase Reaction Stopping Buffer
ReadiUse™ Yeast Cell Lysis Buffer *5X*
ReadiUse™ ATP Reference Standard *10 mM Aqueous Solution*
Show More (61)


Molecular weight
MTS reagent is one of the most common water-soluble tetrazolium reagents that are widely used to quantify cell viability by absorption spectrophotometry. A variety of tetrazolium inner salts are used for determining cell viability in proliferation assays by monitoring their color changes. Metabolically active cells reduce colorless tetrazolium inner salts into the colored formazan products that might be either soluble or insoluble in cell culture media depending on the structures of tetrazolium reagents. The conversion of tetrazolium inner salts to formazans is thought to be carried out by NAD(P)H-dependent dehydrogenase enzymes.


Absorbance microplate reader

Absorbance490 nm
Recommended plateClear bottom


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of ReadiUse™ MTS Reagent *5 mM aqueous solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM205.124 µL1.026 mL2.051 mL10.256 mL20.512 mL
5 mM41.025 µL205.124 µL410.248 µL2.051 mL4.102 mL
10 mM20.512 µL102.562 µL205.124 µL1.026 mL2.051 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles



View all 39 references: Citation Explorer
The edge effect: A global problem. The trouble with culturing cells in 96-well plates.
Authors: Mansoury, Morva and Hamed, Maya and Karmustaji, Rashid and Al Hannan, Fatima and Safrany, Stephen T
Journal: Biochemistry and biophysics reports (2021): 100987
Profiling the Neoplasm Microenvironment of Silica Nanomaterial-Derived Scaffolds of Single, 2-, and 3-Composite Systems.
Authors: Akpe, Victor and Murhekar, Shweta and Kim, Tak H and Brown, Christopher L and Cock, Ian E
Journal: Assay and drug development technologies (2021): 191-203
A Modified MTS Proliferation Assay for Suspended Cells to Avoid the Interference by Hydralazine and β-Mercaptoethanol.
Authors: Wang, Yutang and Nguyen, Dinh Tam and Yang, Guang and Anesi, Jack and Kelly, Jason and Chai, Zhonglin and Ahmady, Fahima and Charchar, Fadi and Golledge, Jonathan
Journal: Assay and drug development technologies (2021): 184-190
Synthesis, structural characterization, and prospects for new cobalt (II) complexes with thiocarbamoyl-pyrazoline ligands as promising antifungal agents.
Authors: Dias, Bianca Boni and da Silva Dantas, Fabiana Gomes and Galvão, Fernanda and Cupozak-Pinheiro, Wellinton Jhon and Wender, Heberton and Pizzuti, Lucas and Rosa, Persiely Pires and Tenório, Kátia Veronica and Gatto, Claudia Cristina and Negri, Melyssa and Casagrande, Gleison Antônio and de Oliveira, Kelly Mari Pires
Journal: Journal of inorganic biochemistry (2020): 111277
The Predictive Value of Estrogen Receptor 1 on Adjuvant Chemotherapy in Locally Advanced Colorectal Cancer: A Retrospective Analysis With Independent Validation and Its Potential Mechanism.
Authors: Ye, Shu-Biao and Cheng, Yi-Kan and Deng, Ru and Deng, Yanhong and Li, Peisi and Zhang, Lin and Lan, Ping
Journal: Frontiers in oncology (2020): 214
Calcineurin/NFAT Signaling Modulates Pulmonary Artery Smooth Muscle Cell Proliferation, Migration and Apoptosis in Monocrotaline-Induced Pulmonary Arterial Hypertension Rats.
Authors: He, Rui-Lan and Wu, Zhi-Juan and Liu, Xiao-Ru and Gui, Long-Xin and Wang, Rui-Xing and Lin, Mo-Jun
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2018): 172-189
Glutathione release through connexin hemichannels: Implications for chemical modification of pores permeable to large molecules.
Authors: Tong, Xuhui and Lopez, William and Ramachandran, Jayalakshmi and Ayad, Wafaa A and Liu, Yu and Lopez-Rodriguez, Angelica and Harris, Andrew L and Contreras, Jorge E
Journal: The Journal of general physiology (2015): 245-54
Caution is required in interpretation of mutations in the voltage sensing domain of voltage gated channels as evidence for gating mechanisms.
Authors: Kariev, Alisher M and Green, Michael E
Journal: International journal of molecular sciences (2015): 1627-43
Cysteine accessibility probes timing and extent of NBD separation along the dimer interface in gating CFTR channels.
Authors: Chaves, Luiz A Poletto and Gadsby, David C
Journal: The Journal of general physiology (2015): 261-83
CHARMM-GUI PDB manipulator for advanced modeling and simulations of proteins containing nonstandard residues.
Authors: Jo, Sunhwan and Cheng, Xi and Islam, Shahidul M and Huang, Lei and Rui, Huan and Zhu, Allen and Lee, Hui Sun and Qi, Yifei and Han, Wei and Vanommeslaeghe, Kenno and MacKerell, Alexander D and Roux, Benoît and Im, Wonpil
Journal: Advances in protein chemistry and structural biology (2014): 235-65