ReadiUse™ RBC Lysis Buffer is a ready-to-use 1X solution optimized for the selective lysis of red blood cells (RBCs) in single-cell suspensions while preserving the viability and functionality of leukocytes. This buffer is specifically designed for processing mouse hematopoietic tissues, such as spleen, and human peripheral blood samples, making it suitable for a wide range of immunology, hematology, and flow cytometry applications.

The formulation contains ammonium chloride, which induces osmotic lysis of erythrocytes while exerting minimal effects on lymphocytes and other nucleated white blood cells when used as recommended. However, nucleated red blood cells are not effectively lysed by ammonium chloride and may require alternative or additional treatment.

ReadiUse™ RBC Lysis Buffer is supplied as a pre-diluted, ready-to-use solution, eliminating the need for preparation steps and ensuring reproducibility across experiments. This reagent has been rigorously tested to provide efficient RBC removal with minimal leukocyte loss, making it an ideal choice for downstream applications requiring high-purity immune cell populations.