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ReadiUse™ TCA Deproteinization Sample Preparation Kit
The analysis of amino acids, ions, metabolites and other small molecules is frequently hindered by the interference of lipids, protein and enzymes present in biofluids, cell and tissue lysates. Therefore, deproteinization is a necessary step in many procedures prior to analysis of biological samples. AAT Bioquest's Deproteinization Sample Preparation Kit offers a rapid method for removing proteins in biological samples. Proteins are precipitated by applying trichloroacetic acid (TCA). After removal of precipitated proteins, the pH of the sample is neutralized with neutralization solution. TCA based deproteinization has been successfully and widely used in sample preparation prior to quantitation of small molecules, such as glycogen, ATP, cAMP, glutathione and antioxidants. The TCA method provided in this kit can also be used for removing protein in large scale. Biological samples prepared using this kit can be directly used in various biochemical analysis.
TCA-based deproteinization of protein samples. Bovine serum albumin (BSA) samples with protein concentration less than 50 mg/mL and a rabbit serum sample at the concentration about 14.2 mg/mL were deproteinized using ReadiUse™ TCA Deproteinization Test Sample Preparation Kit (Cat#19501). More than 98% of protein in all samples was removed with TCA method.
TCA-based deproteinization of protein samples. Bovine serum albumin (BSA) samples with protein concentration less than 50 mg/mL and a rabbit serum sample at the concentration about 14.2 mg/mL were deproteinized using ReadiUse™ TCA Deproteinization Test Sample Preparation Kit (Cat#19501). More than 98% of protein in all samples was removed with TCA method.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
19501200 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
UNSPSC41116134
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 29, 2025