Name: ReadiUse™ TCA Deproteinization Sample Preparation Kit
Brand: AAT Bioquest
SKU: 19501
Price: 178 USD
Availability: InStock

ReadiUse™ TCA Deproteinization Sample Preparation Kit

ReadiUse™ TCA Deproteinization Sample Preparation Kit is a sample preparation kit for rapidly removing proteins from biological samples prior to small-molecule analysis.

Deproteinization Method: Uses trichloroacetic acid (TCA) to precipitate proteins.
Removal Efficiency: Removes more than 98% of protein in AAT’s demonstrated BSA and rabbit serum samples.
Sample Compatibility: Suitable for biofluids, cell lysates, and tissue lysates.
Applications: Supports analysis of small molecules including glycogen, ATP, cAMP, glutathione, and antioxidants.

TCA-based deproteinization of protein samples. Bovine serum albumin (BSA) samples with protein concentration less than 50 mg/mL and a rabbit serum sample at the concentration about 14.2 mg/mL were deproteinized using ReadiUse™ TCA Deproteinization Test Sample Preparation Kit (Cat#19501). More than 98% of protein in all samples was removed with TCA method.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
19501	200 Tests	Price

Overview

The analysis of amino acids, ions, metabolites and other small molecules is frequently hindered by the interference of lipids, protein and enzymes present in biofluids, cell and tissue lysates. Therefore, deproteinization is a necessary step in many procedures prior to analysis of biological samples. AAT Bioquest's Deproteinization Sample Preparation Kit offers a rapid method for removing proteins in biological samples. Proteins are precipitated by applying trichloroacetic acid (TCA). After removal of precipitated proteins, the pH of the sample is neutralized with neutralization solution. TCA based deproteinization has been successfully and widely used in sample preparation prior to quantitation of small molecules, such as glycogen, ATP, cAMP, glutathione and antioxidants. The TCA method provided in this kit can also be used for removing protein in large scale. Biological samples prepared using this kit can be directly used in various biochemical analysis.

Citations

View all 5 citations: Citation Explorer

FAD synthase confers ferroptosis resistance and restrains CD8+ T cell recruitment in hepatocellular carcinoma

Authors:

Chao, Jiashuo and Liang, Yuan and Wang, Hao and Xun, Ziyu and Wang, Shanshan and Xuan, Zhengfeng and Wang, Mingming and Huang, Qiyuan and Zhang, Rui and Liu, Mu and others,

Journal:

Nature Communications (2025): 9547

Ionizing radiation induces mild and dose-independent damage to mitochondria in newt cells

Authors:

Hasan, Md Mahmudul and Kawabata, Tsuyoshi and Chen, Yan and Sekiya, Reiko and Goto, Shinji and Urata, Yoshishige and Li, Tao-Sheng

Journal:

Experimental Cell Research (2025): 114575

Increased alcohol dehydrogenase 1 activity promotes longevity

Authors:

Ghaddar, Abbas and Mony, Vinod K and Mishra, Swarup and Berhanu, Samuel and Johnson, James C and Enriquez-Hesles, Elisa and Harrison, Emma and Patel, Aaroh and Horak, Mary Kate and Smith, Jeffrey S and others,

Journal:

Current Biology (2023)

Curcumin induces apoptosis by inhibiting BCAT1 expression and mTOR signaling in cytarabine-resistant myeloid leukemia cells

Authors:

Tseng, Yu-Hsin and Yang, Rei-Cheng and Chiou, Shyh-Shin and Shieh, Tzong-Ming and Shih, Yin-Hwa and Lin, Pei-Chin

Journal:

Molecular Medicine Reports (2021): 1--8

Analysis of the Status of the Cutaneous Endogenous and Exogenous Antioxidative System of Smokers and the Short-Term Effect of Defined Smoking Thereon

Authors:

Lohan, Silke B and B{\"u}hring, Karl and Lauer, Anna-Christina and Friedrich, Annette and Lademann, J{\"u}rgen and Buss, Annette and Sabat, Robert and Wolk, Kerstin and Meinke, Martina C

Journal:

Antioxidants (2020): 537

References

View all 11 references: Citation Explorer

Electrochemical determination of glutathione in plasma at carbon nanotubes based screen printed electrodes

Authors:

Turunc E, Karadeniz H, Armagan G, Erdem A, Yalcin A.

Journal:

Comb Chem High Throughput Screen (2013): 695

Preparation procedures of anti-complementary polysaccharides from Houttuynia cordata

Authors:

Zhang J, Lu Y, Chen D.

Journal:

Zhongguo Zhong Yao Za Zhi (2012): 2071

Extraction and deproteinization of pumpkin polysaccharide

Authors:

Huang G, Chen Y, Wang X.

Journal:

Int J Food Sci Nutr (2011): 568

Study on methods of deproteinization from Phellinus baumii polysaccharide

Authors:

Xie LY, Zhang Y, Peng WH, Gan BC.

Journal:

Zhong Yao Cai (2011): 293

Study on deproteinization and decoloration in extraction of polysaccharides from Rabdosia rubescens

Authors:

Liu F, Zhu XH, Liu M, Zhou J, Che XP, Han RF.

Journal:

Zhong Yao Cai (2008): 751

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on October 28, 2026

Component A: TCA	1 bottle (2 mL)
Component B: Neutralization Solution	1 bottle (2 mL)