ReadiUse™ Tyramide (TSA)/Styramide (PSA) Optimized Reaction Buffer
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12171501 |
Overview | SDSProtocol |
Components
Example protocol
AT A GLANCE
- Fix/permeabilize/block cells or tissue
- Add primary antibody in blocking buffer
- Add HRP-conjugated secondary antibody
- Prepare Styramide™ (PSA) or Tyramide (TSA) working solution and apply in cells or tissue for 5-10 minutes at room temperature
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Add 100 μL of DMSO into the vial of iFluor® dye-labeled Styramide™ conjugate (for example, Cat. 45000) to make 100X Styramide™ stock solution.
Note: Make single-use aliquots, and store unused 100X stock solution at 2-8 °C in a dark place and avoid repeat freeze-thaw cycles.
Add 10 μL of 3% hydrogen peroxide (Component B) to 90 μL of ddH2O.
Note: Prepare the 100X H2O2 solution fresh on the day of use.
PREPARATION OF WORKING SOLUTION
Every 1 mL of PSA working solution requires 1 mL TSA/PSA buffer (Component A), 10 μL of Styramide™ stock solution, and 10 μL of H2O2 stock solution.
Note: 1 mL is enough for 10 tests based on 100 μL of Styramide™ working solution needed per coverslip or per well in a 96-well microplate.
Note: The Styramide™ working solution must be used within 2 hours after preparation and avoid direct exposure to light.
Citations
Authors: Molina, Catalina Pineda
Journal: (2018)
References
Authors: Prajsnar, Tomasz K and Serba, Justyna J and Dekker, Bernice M and Gibson, Josie F and Masud, Samrah and Fleming, Angeleen and Johnston, Simon A and Renshaw, Stephen A and Meijer, Annemarie H
Journal: Autophagy (2021): 888-902
Authors: Gallego, Sara and Barkay, Tamar and Fahrenfeld, N L
Journal: The Science of the total environment (2020): 138865
Authors: Chen, Haihong and Feng, Xiaoqing and Jiang, Minjie and Xiao, Baoheng and Zhang, Jingyu and Zhang, Wei and Hu, Yiyi and Sui, Zhenghong
Journal: Journal of phycology (2020): 1339-1348
Authors: Buescher, Marita and Oberhofer, Georg and Garcia-Perez, Natalia Carolina and Bucher, Gregor
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 219-232
Authors: Wang, Herui and Pangilinan, Ryan L and Zhu, Yan
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 89-97
Authors: Roberts, Kenny and Bayraktar, Omer Ali
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 229-244
Authors: Parra, Edwin Roger and Jiang, Mei and Solis, Luisa and Mino, Barbara and Laberiano, Caddie and Hernandez, Sharia and Gite, Swati and Verma, Anuj and Tetzlaff, Michael and Haymaker, Cara and Tamegnon, Auriole and Rodriguez-Canales, Jaime and Hoyd, Clifford and Bernachez, Chantale and Wistuba, Ignacio
Journal: Cancers (2020)
Authors: Lauter, Gilbert and Söll, Iris and Hauptmann, Giselbert
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 397-409
Authors: Tan-Garcia, Alfonso and Lai, Fritz and Sheng Yeong, Joe Poh and Irac, Sergio E and Ng, Pei Y and Msallam, Rasha and Tatt Lim, Jeffrey Chun and Wai, Lu-En and Tham, Christine Y L and Choo, Su P and Lim, Tony and Young, Dan Y and D'Ambrosio, Roberta and Degasperi, Elisabetta and Perbellini, Riccardo and Newell, Evan and Le Bert, Nina and Ginhoux, Florent and Bertoletti, Antonio and Chen, Qingfeng and Dutertre, Charles-Antoine
Journal: JHEP reports : innovation in hepatology (2020): 100062
Authors: Masud, Samrah and Prajsnar, Tomasz K and Torraca, Vincenzo and Lamers, Gerda E M and Benning, Marianne and Van Der Vaart, Michiel and Meijer, Annemarie H
Journal: Autophagy (2019): 796-812
Application notes
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