ReadiUse™ Tyramide (TSA)/Styramide (PSA) Optimized Reaction Buffer

1. Fix/permeabilize/block cells or tissue
2. Add primary antibody in blocking buffer
3. Add HRP-conjugated secondary antibody
4. Prepare Styramide™ (PSA) or Tyramide (TSA) working solution and apply in cells or tissue for 5-10 minutes at room temperature

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles

Add 100 μL of DMSO into the vial of iFluor® dye-labeled Styramide™ conjugate (for example, Cat. 45000) to make 100X Styramide™ stock solution.

Note: Make single-use aliquots, and store unused 100X stock solution at 2-8 °C in a dark place and avoid repeat freeze-thaw cycles.

Add 10 μL of 3% hydrogen peroxide (Component B) to 90 μL of ddH₂O.

Note: Prepare the 100X H₂O₂ solution fresh on the day of use.

Every 1 mL of PSA working solution requires 1 mL TSA/PSA buffer (Component A), 10 μL of Styramide™ stock solution, and 10 μL of H₂O₂ stock solution.

Note: 1 mL is enough for 10 tests based on 100 μL of Styramide™ working solution needed per coverslip or per well in a 96-well microplate.

Note: The Styramide™ working solution must be used within 2 hours after preparation and avoid direct exposure to light.