ReadiUse™ Tyramide (TSA)/Styramide (PSA) Optimized Reaction Buffer
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
| Certificate of Origin | Download PDF |
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| UNSPSC | 12171501 |
Related products
| Overview |  SDSProtocol |
See also: Buffers and Lab Consumables
For many immunohistochemical (IHC) applications, the traditional enzymatic amplification procedures are sufficient for achieving adequate antigen detection. However, several factors limit the sensitivity and utility of these procedures. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. Power Styramide™ Signal Amplification (PSA™) is a recent upgrade to the TSA system with improved fluorescence signal 10-50 times higher than the corresponding TSA reagents. This HRP-induced radical reaction buffer is optimized for running both TSA and PSA reactions.
Components
Example protocol
AT A GLANCE
Protocol summary
- Fix/permeabilize/block cells or tissue
- Add primary antibody in blocking buffer
- Add HRP-conjugated secondary antibody
- Prepare Styramide™ (PSA) or Tyramide (TSA) working solution and apply in cells or tissue for 5-10 minutes at room temperature
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
Note Make single use aliquots, and store unused 100X stock solution at 2-8 °C in dark place and avoid repeat freeze-thaw cycles.
Note Prepare the 100X H2O2 solution fresh on the day of use.
1. Styramide™ (PSA) stock solution (100X)
Add 100 μL of DMSO into the vial of iFluor™ dye-labeled Styramide™ conjugate (for example cat#45000) to make 100X Styramide™ stock solution.Note Make single use aliquots, and store unused 100X stock solution at 2-8 °C in dark place and avoid repeat freeze-thaw cycles.
2. H2O2 stock solution
Add 10 μL of 3% hydrogen peroxide (Component B) to 90 μL of ddH2O.Note Prepare the 100X H2O2 solution fresh on the day of use.
PREPARATION OF WORKING SOLUTION
Styramide™ (PSA) working solution (1X)
Every 1 mL of PSA working solution requires 1 mL TSA/PSA buffer (Component A), 10 μL of Styramide™ stock solution and 10 μL of H2O2 stock solution.Note 1 mL is enough for 10 tests based on 100 μL of Styramide™ working solution needed per coverslip or per well in a 96-well microplate.
Note The Styramide™ working solution must be used within 2 hours after preparation and avoid direct exposure to light.
Citations
View all 1 citations: Citation Explorer
Characterization of the Host Tissue Response Induced by a Biosynthetic Material Composed of Poly (4-Hydroxybutyrate)
Authors: Molina, Catalina Pineda
Journal: (2018)
Authors: Molina, Catalina Pineda
Journal: (2018)
References
View all 50 references: Citation Explorer
The autophagic response to Staphylococcus aureus provides an intracellular niche in neutrophils.
Authors: Prajsnar, Tomasz K and Serba, Justyna J and Dekker, Bernice M and Gibson, Josie F and Masud, Samrah and Fleming, Angeleen and Johnston, Simon A and Renshaw, Stephen A and Meijer, Annemarie H
Journal: Autophagy (2021): 888-902
Authors: Prajsnar, Tomasz K and Serba, Justyna J and Dekker, Bernice M and Gibson, Josie F and Masud, Samrah and Fleming, Angeleen and Johnston, Simon A and Renshaw, Stephen A and Meijer, Annemarie H
Journal: Autophagy (2021): 888-902
Tagging the vanA gene in wastewater microbial communities for cell sorting and taxonomy of vanA carrying cells.
Authors: Gallego, Sara and Barkay, Tamar and Fahrenfeld, N L
Journal: The Science of the total environment (2020): 138865
Authors: Gallego, Sara and Barkay, Tamar and Fahrenfeld, N L
Journal: The Science of the total environment (2020): 138865
Estimating the ploidy of Gracilariopsis lemaneiformis at both the cellular and genomic level1.
Authors: Chen, Haihong and Feng, Xiaoqing and Jiang, Minjie and Xiao, Baoheng and Zhang, Jingyu and Zhang, Wei and Hu, Yiyi and Sui, Zhenghong
Journal: Journal of phycology (2020): 1339-1348
Authors: Chen, Haihong and Feng, Xiaoqing and Jiang, Minjie and Xiao, Baoheng and Zhang, Jingyu and Zhang, Wei and Hu, Yiyi and Sui, Zhenghong
Journal: Journal of phycology (2020): 1339-1348
A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum.
Authors: Buescher, Marita and Oberhofer, Georg and Garcia-Perez, Natalia Carolina and Bucher, Gregor
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 219-232
Authors: Buescher, Marita and Oberhofer, Georg and Garcia-Perez, Natalia Carolina and Bucher, Gregor
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 219-232
Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence.
Authors: Wang, Herui and Pangilinan, Ryan L and Zhu, Yan
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 89-97
Authors: Wang, Herui and Pangilinan, Ryan L and Zhu, Yan
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 89-97
Automation of Multiplexed RNAscope Single-Molecule Fluorescent In Situ Hybridization and Immunohistochemistry for Spatial Tissue Mapping.
Authors: Roberts, Kenny and Bayraktar, Omer Ali
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 229-244
Authors: Roberts, Kenny and Bayraktar, Omer Ali
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 229-244
Procedural Requirements and Recommendations for Multiplex Immunofluorescence Tyramide Signal Amplification Assays to Support Translational Oncology Studies.
Authors: Parra, Edwin Roger and Jiang, Mei and Solis, Luisa and Mino, Barbara and Laberiano, Caddie and Hernandez, Sharia and Gite, Swati and Verma, Anuj and Tetzlaff, Michael and Haymaker, Cara and Tamegnon, Auriole and Rodriguez-Canales, Jaime and Hoyd, Clifford and Bernachez, Chantale and Wistuba, Ignacio
Journal: Cancers (2020)
Authors: Parra, Edwin Roger and Jiang, Mei and Solis, Luisa and Mino, Barbara and Laberiano, Caddie and Hernandez, Sharia and Gite, Swati and Verma, Anuj and Tetzlaff, Michael and Haymaker, Cara and Tamegnon, Auriole and Rodriguez-Canales, Jaime and Hoyd, Clifford and Bernachez, Chantale and Wistuba, Ignacio
Journal: Cancers (2020)
Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish.
Authors: Lauter, Gilbert and Söll, Iris and Hauptmann, Giselbert
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 397-409
Authors: Lauter, Gilbert and Söll, Iris and Hauptmann, Giselbert
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 397-409
Liver fibrosis and CD206+ macrophage accumulation are suppressed by anti-GM-CSF therapy.
Authors: Tan-Garcia, Alfonso and Lai, Fritz and Sheng Yeong, Joe Poh and Irac, Sergio E and Ng, Pei Y and Msallam, Rasha and Tatt Lim, Jeffrey Chun and Wai, Lu-En and Tham, Christine Y L and Choo, Su P and Lim, Tony and Young, Dan Y and D'Ambrosio, Roberta and Degasperi, Elisabetta and Perbellini, Riccardo and Newell, Evan and Le Bert, Nina and Ginhoux, Florent and Bertoletti, Antonio and Chen, Qingfeng and Dutertre, Charles-Antoine
Journal: JHEP reports : innovation in hepatology (2020): 100062
Authors: Tan-Garcia, Alfonso and Lai, Fritz and Sheng Yeong, Joe Poh and Irac, Sergio E and Ng, Pei Y and Msallam, Rasha and Tatt Lim, Jeffrey Chun and Wai, Lu-En and Tham, Christine Y L and Choo, Su P and Lim, Tony and Young, Dan Y and D'Ambrosio, Roberta and Degasperi, Elisabetta and Perbellini, Riccardo and Newell, Evan and Le Bert, Nina and Ginhoux, Florent and Bertoletti, Antonio and Chen, Qingfeng and Dutertre, Charles-Antoine
Journal: JHEP reports : innovation in hepatology (2020): 100062
Macrophages target Salmonella by Lc3-associated phagocytosis in a systemic infection model.
Authors: Masud, Samrah and Prajsnar, Tomasz K and Torraca, Vincenzo and Lamers, Gerda E M and Benning, Marianne and Van Der Vaart, Michiel and Meijer, Annemarie H
Journal: Autophagy (2019): 796-812
Authors: Masud, Samrah and Prajsnar, Tomasz K and Torraca, Vincenzo and Lamers, Gerda E M and Benning, Marianne and Van Der Vaart, Michiel and Meijer, Annemarie H
Journal: Autophagy (2019): 796-812