Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

ReadiUse™ Tyramide (TSA)/Styramide (PSA) Optimized Reaction Buffer

Ordering information
Price ()
Catalog Number45090
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Related products
ReadiUse™ Preactivated APC-iFluor® 700 Tandem
ReadiUse™ Preactivated APC-iFluor® 750 Tandem
ReadiUse™ Preactivated PE-iFluor® 647 Tandem
ReadiUse™ Preactivated PE-iFluor® 750 Tandem
ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
ReadiUse™ hydrogen peroxide solution *50 mM calibrated and stabilized solution*
ReadiUse™ Preactivated HRP NHS ester
ReadiUse™ NADPH Regenerating Kit
ReadiUse™ NADP Regenerating Kit
ReadiUse™ TCA Deproteinization Sample Preparation Kit
ReadiUse™ microscope mounting solution
ReadiUse™ 4% formaldehyde fixation solution
ReadiUse™ mammalian cell lysis buffer *5X*
ReadiUse™ probenecid, sodium salt *Water-soluble*
ReadiUse™ probenecid *25 mM stabilized aqueous solution*
ReadiUse™ CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *CAS 150347-59-4*
ReadiUse™ Preactivated PE
ReadiUse™ Preactivated APC
ReadiUse™ Preactivated PE-Cy5 Tandem
ReadiUse™ Preactivated PE-Cy5.5 Tandem
ReadiUse™ Preactivated PE-Cy7 Tandem
ReadiUse™ Preactivated PE-Texas Red Tandem
ReadiUse™ Preactivated APC-Cy5.5 Tandem
ReadiUse™ Preactivated APC-Cy7 Tandem
ReadiUse™ TCEP removal solution
ReadiUse™ WST-8 *50 mM aqueous solution*
ReadiUse™ Rapid Luminometric ATP Assay Kit
ReadiUse™ PE [R-Phycoerythrin] *Ammonium Sulfate-Free*
ReadiUse™ CL-APC [Cross linked-Allophycocyanin] *Ammonium Sulfate-Free*
ReadiUse™ Preactivated PE-iFluor® 594 Tandem
ReadiUse™ Cell Detaching Buffer
ReadiUse™ DRAQ5 Staining Solution *5 mM in Water*
ReadiUse™ Staurosporine * 1 mM DMSO stock solution*
ReadiUse™ Bio-Gel P-6 spin column
ReadiUse™ bacterial cell lysis buffer *5X*
ReadiUse™ 10% Triton X-100 *Hydrogen Peroxide-and Carbonyl-Free*
ReadiUse™ Preactivated PE-iFluor® 700 Tandem
ReadiUse™ dNTP Mix *10 mM*
ReadiUse™ Viral RNA Lysis Buffer
ReadiUse™ Preactivated PerCP
ReadiUse™ Preactivated PerCP-Cy5.5 Tandem
ReadiUse™ Preactivated PE-iFluor® 660 Tandem
ReadiUse™ 10KD Spin Filter
ReadiUse™ Preactivated APC-iFluor® 800 Tandem
ReadiUse™ 1 Kb Plus DNA Ladder
ReadiUse™ GeneRuler 1 kb DNA Ladder
ReadiUse™ 100 bp DNA Ladder
ReadiUse™ ddNTP Terminator Mix *10 mM*
ReadiUse™ dNTP Mix Set *10 mM PCR Grade*
ReadiUse™ Preactivated HRP maleimide
ReadiUse™ Stayright™ Purple *HRP Chromogen Premixed with Hydrogen Peroxide*
ReadiUse™ Disposable PD-10 Desalting Column
ReadiUse™ Preactivated APC Maleimide [Activated Allophycocyanin]
ReadiUse™ Preactivated PE Maleimide [Activated R-Phycoerythrin]
ReadiUse™ 6-Color Human TBNK Antibody Kit *Dry Reagent Format*
ReadiUse™ MTS Reagent *50 mM aqueous solution*
ReadiUse™ Preactivated PE-Texas Red Maleimide
ReadiUse™ Preactivated PE-Cy5 Maleimide
ReadiUse™ Preactivated PE-Cy5.5 Maleimide
ReadiUse™ Preactivated PE-Cy7 Maleimide
ReadiUse™ Preactivated APC-AF700 Maleimide
ReadiUse™ Preactivated APC-Cy5.5 Maleimide
ReadiUse™ Preactivated APC-Cy7 Maleimide
ReadiUse™ Preactivated PerCP Maleimide
ReadiUse™ Preactivated PerCP-Cy5.5 Maleimide
ReadiUse™ Preactivated PerCP-Cy7 Maleimide
Show More (67)

OverviewpdfSDSpdfProtocol


For many immunohistochemical (IHC) applications, the traditional enzymatic amplification procedures are sufficient for achieving adequate antigen detection. However, several factors limit the sensitivity and utility of these procedures. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. Power Styramide™ Signal Amplification (PSA™) is a recent upgrade to the TSA system with improved fluorescence signal 10-50 times higher than the corresponding TSA reagents. This HRP-induced radical reaction buffer is optimized for running both TSA and PSA reactions.

Components


Component A: TSA/PSA buffer1 bottle (100 mL)
Component B: Stabilized 3% Hydrogen peroxide 1 vial (100 µL)

Example protocol


AT A GLANCE

Protocol summary
  1. Fix/permeabilize/block cells or tissue
  2. Add primary antibody in blocking buffer
  3. Add HRP-conjugated secondary antibody
  4. Prepare Styramide™ (PSA) or Tyramide (TSA) working solution and apply in cells or tissue for 5-10 minutes at room temperature 

PREPARATION OF STOCK SOLUTIONS

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

1. Styramide™ (PSA) stock solution (100X)
Add 100 μL of DMSO into the vial of iFluor™ dye-labeled Styramide™ conjugate (for example cat#45000) to make 100X Styramide™ stock solution.
Note     Make single use aliquots, and store unused 100X stock solution at 2-8 °C in dark place and avoid repeat freeze-thaw cycles.


2. H2O2 stock solution
Add 10 μL of 3% hydrogen peroxide (Component B) to 90 μL of ddH2O.
Note     Prepare the 100X H2O2 solution fresh on the day of use.

PREPARATION OF WORKING SOLUTION

Styramide™ (PSA) working solution (1X)
Every 1 mL of PSA working solution requires 1 mL TSA/PSA buffer (Component A), 10 μL of Styramide™ stock solution and 10 μL of H2O2 stock solution.
Note     1 mL is enough for 10 tests based on 100 μL of Styramide™ working solution needed per coverslip or per well in a 96-well microplate.
Note     The Styramide™ working solution must be used within 2 hours after preparation and avoid direct exposure to light.

Citations


View all 1 citations: Citation Explorer
Characterization of the Host Tissue Response Induced by a Biosynthetic Material Composed of Poly (4-Hydroxybutyrate)
Authors: Molina, Catalina Pineda
Journal: (2018)

References


View all 50 references: Citation Explorer
The autophagic response to Staphylococcus aureus provides an intracellular niche in neutrophils.
Authors: Prajsnar, Tomasz K and Serba, Justyna J and Dekker, Bernice M and Gibson, Josie F and Masud, Samrah and Fleming, Angeleen and Johnston, Simon A and Renshaw, Stephen A and Meijer, Annemarie H
Journal: Autophagy (2021): 888-902
Tagging the vanA gene in wastewater microbial communities for cell sorting and taxonomy of vanA carrying cells.
Authors: Gallego, Sara and Barkay, Tamar and Fahrenfeld, N L
Journal: The Science of the total environment (2020): 138865
Estimating the ploidy of Gracilariopsis lemaneiformis at both the cellular and genomic level1.
Authors: Chen, Haihong and Feng, Xiaoqing and Jiang, Minjie and Xiao, Baoheng and Zhang, Jingyu and Zhang, Wei and Hu, Yiyi and Sui, Zhenghong
Journal: Journal of phycology (2020): 1339-1348
A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum.
Authors: Buescher, Marita and Oberhofer, Georg and Garcia-Perez, Natalia Carolina and Bucher, Gregor
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 219-232
Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence.
Authors: Wang, Herui and Pangilinan, Ryan L and Zhu, Yan
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 89-97
Automation of Multiplexed RNAscope Single-Molecule Fluorescent In Situ Hybridization and Immunohistochemistry for Spatial Tissue Mapping.
Authors: Roberts, Kenny and Bayraktar, Omer Ali
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 229-244
Procedural Requirements and Recommendations for Multiplex Immunofluorescence Tyramide Signal Amplification Assays to Support Translational Oncology Studies.
Authors: Parra, Edwin Roger and Jiang, Mei and Solis, Luisa and Mino, Barbara and Laberiano, Caddie and Hernandez, Sharia and Gite, Swati and Verma, Anuj and Tetzlaff, Michael and Haymaker, Cara and Tamegnon, Auriole and Rodriguez-Canales, Jaime and Hoyd, Clifford and Bernachez, Chantale and Wistuba, Ignacio
Journal: Cancers (2020)
Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish.
Authors: Lauter, Gilbert and Söll, Iris and Hauptmann, Giselbert
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 397-409
Liver fibrosis and CD206+ macrophage accumulation are suppressed by anti-GM-CSF therapy.
Authors: Tan-Garcia, Alfonso and Lai, Fritz and Sheng Yeong, Joe Poh and Irac, Sergio E and Ng, Pei Y and Msallam, Rasha and Tatt Lim, Jeffrey Chun and Wai, Lu-En and Tham, Christine Y L and Choo, Su P and Lim, Tony and Young, Dan Y and D'Ambrosio, Roberta and Degasperi, Elisabetta and Perbellini, Riccardo and Newell, Evan and Le Bert, Nina and Ginhoux, Florent and Bertoletti, Antonio and Chen, Qingfeng and Dutertre, Charles-Antoine
Journal: JHEP reports : innovation in hepatology (2020): 100062
Macrophages target Salmonella by Lc3-associated phagocytosis in a systemic infection model.
Authors: Masud, Samrah and Prajsnar, Tomasz K and Torraca, Vincenzo and Lamers, Gerda E M and Benning, Marianne and Van Der Vaart, Michiel and Meijer, Annemarie H
Journal: Autophagy (2019): 796-812