ReadiUse™ Tyramide (TSA)/Styramide™ (PSA) Optimized Reaction Buffer

ReadiUse™ Tyramide (TSA)/Styramide (PSA) Optimized Reaction Buffer is an HRP-induced radical reaction buffer optimized for tyramide and Styramide™ signal amplification in cells and tissues.

Detection Principle: Supports HRP/H₂O₂-mediated formation of reactive tyramide or Styramide™ radicals.
Assay Compatibility: Optimized for both TSA and PSA™ signal amplification reactions.
Workflow: Working solution is applied to cells or tissues for 5–10 minutes at room temperature.

General packaging for Catalog 45090. Your actual packaging may vary.

Protocol

SDS

Catalog	Size	Price	Quantity
45090	1000 Reactions	Price

Overview

For many immunohistochemical (IHC) applications, the traditional enzymatic amplification procedures are sufficient for achieving adequate antigen detection. However, several factors limit the sensitivity and utility of these procedures. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. Power Styramide™ Signal Amplification (PSA™) is a recent upgrade to the TSA system with improved fluorescence signal 10-50 times higher than the corresponding TSA reagents. This HRP-induced radical reaction buffer is optimized for running both TSA and PSA™ reactions.

References

View all 50 references: Citation Explorer

The autophagic response to Staphylococcus aureus provides an intracellular niche in neutrophils.

Authors:

Prajsnar, Tomasz K and Serba, Justyna J and Dekker, Bernice M and Gibson, Josie F and Masud, Samrah and Fleming, Angeleen and Johnston, Simon A and Renshaw, Stephen A and Meijer, Annemarie H

Journal:

Autophagy (2021): 888-902

Tagging the vanA gene in wastewater microbial communities for cell sorting and taxonomy of vanA carrying cells.

Authors:

Gallego, Sara and Barkay, Tamar and Fahrenfeld, N L

Journal:

The Science of the total environment (2020): 138865

Estimating the ploidy of Gracilariopsis lemaneiformis at both the cellular and genomic level1.

Authors:

Chen, Haihong and Feng, Xiaoqing and Jiang, Minjie and Xiao, Baoheng and Zhang, Jingyu and Zhang, Wei and Hu, Yiyi and Sui, Zhenghong

Journal:

Journal of phycology (2020): 1339-1348

A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum.

Authors:

Buescher, Marita and Oberhofer, Georg and Garcia-Perez, Natalia Carolina and Bucher, Gregor

Journal:

Methods in molecular biology (Clifton, N.J.) (2020): 219-232

Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence.

Authors:

Wang, Herui and Pangilinan, Ryan L and Zhu, Yan

Journal:

Methods in molecular biology (Clifton, N.J.) (2020): 89-97

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on October 15, 2026

Component A: TSA/PSA Buffer	1 bottle (100 mL)
Component B: Stabilized 3% Hydrogen peroxide	1 vial (100 µL)