ReadiUse™ Viral RNA Lysis Buffer

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	N/A
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

N/A

Current demands for RT-PCR testing of SARS-CoV-2 have led to a worldwide shortage of RNA extraction kits. A key missing component required by extraction kit manufacturers and hospitals is the RNA Lysis buffer. This ReadiUse™ Viral RNA Lysis Buffer can be used as an alternative to the Roche MagNA Pure 96 External Lysis Buffer (Code: 06374913001), Biomérieux NucliSENS Lysis Buffer (Code: 280134), and Qiagen Buffer RNAeasy Lysis Buffer (Code: 79216).

Example protocol

PREPARATION OF WORKING SOLUTION

ReadiUse™ Viral RNA Lysis Buffer working solution

Add 0.5 mL of β-Mercaptoethanol to 50 mL of ReadiUse™ Viral RNA Lysis Buffer

Note: Store the RNA Lysis Buffer containing β-Mercaptoethanol at 4 °C.

SAMPLE EXPERIMENTAL PROTOCOL

The following protocol can be used as a guideline and should be optimized according to your experimental needs. This protocol was optimized for use with at least 1.5 X 10³ cells to a maximum of 5 X 10⁶ cells per purification.

Wash the cells once with sterile 1X PBS.
Add 100 µL of ReadiUse™ Viral RNA Lysis Buffer working solution to the washed cells. Mix by pipetting.
Purify extracted RNA from the previous step using RNA purification method of choice.

References

View all 50 references: Citation Explorer

Cell Lysis Based on an Oscillating Microbubble Array.
Authors: Liu, Xiufang and Li, Jinyuan and Zhang, Liangyu and Huang, Xiaowei and Farooq, Umar and Pang, Na and Zhou, Wei and Qi, Lin and Xu, Lisheng and Niu, Lili and Meng, Long
Journal: Micromachines (2020)

PTPN21-CDSlong isoform inhibits the response of acute lymphoblastic leukemia cells to NK-mediated lysis via the KIR/HLA-I axis.
Authors: Wang, Huafang and Zhu, Ni and Ye, Xiaohang and Wang, Limengmeng and Wang, Binsheng and Shan, Wei and Lai, Xiaoyu and Tan, Yamin and Fu, Shan and Xiao, Haowen and Huang, He
Journal: Journal of cellular biochemistry (2020): 3298-3312

Lysis of a Lactococcus lactis Dipeptidase Mutant and Rescue by Mutation in the Pleiotropic Regulator CodY.
Authors: Huang, Chenxi and Hernandez-Valdes, Jhonatan A and Kuipers, Oscar P and Kok, Jan
Journal: Applied and environmental microbiology (2020)

Schistosoma mansoni glyceraldehyde-3-phosphate dehydrogenase enhances formation of the blood-clot lysis protein plasmin.
Authors: Pirovich, David B and Da'dara, Akram A and Skelly, Patrick J
Journal: Biology open (2020)

Transcriptomic responses of the marine cyanobacterium Prochlorococcus to viral lysis products.
Authors: Fang, Xiaoting and Liu, Yaxin and Zhao, Yao and Chen, Yue and Liu, Riyue and Qin, Qi-Long and Li, Gang and Zhang, Yu-Zhong and Chan, Wan and Hess, Wolfgang R and Zeng, Qinglu
Journal: Environmental microbiology (2019): 2015-2028

Extended direct lysis method for virus detection on berries including droplet digital RT-PCR or real time RT-PCR with reduced influence from inhibitors.
Authors: Sun, Baojian and Bosch, Albert and Myrmel, Mette
Journal: Journal of virological methods (2019): 113638

scFTD-seq: freeze-thaw lysis based, portable approach toward highly distributed single-cell 3' mRNA profiling.
Authors: Dura, Burak and Choi, Jin-Young and Zhang, Kerou and Damsky, William and Thakral, Durga and Bosenberg, Marcus and Craft, Joe and Fan, Rong
Journal: Nucleic acids research (2019): e16

Comparison of the efficiency of different cell lysis methods and different commercial methods for RNA extraction from Candida albicans stored in RNAlater.
Authors: Rodríguez, Antonio and Vaneechoutte, Mario
Journal: BMC microbiology (2019): 94

High-resolution studies of lysis-lysogeny decision-making in bacteriophage lambda.
Authors: Shao, Qiuyan and Trinh, Jimmy T and Zeng, Lanying
Journal: The Journal of biological chemistry (2019): 3343-3349

Epithelial ovarian cancer stem‑like cells are resistant to the cellular lysis of cytokine‑induced killer cells via HIF1A‑mediated downregulation of ICAM‑1.
Authors: Bu, Shixia and Li, Boning and Wang, Qian and Gu, Tingting and Dong, Qianggang and Miao, Xiaofei and Lai, Dongmei
Journal: International journal of oncology (2019): 179-190