ReadiUse™ Viral RNA Lysis Buffer
|Shipping||Standard overnight for United States, inquire for international|
|H-phrase||H303, H313, H333|
|Intended use||Research Use Only (RUO)|
|R-phrase||R20, R21, R22|
|Storage||Refrigerated (2-8 °C); Minimize light exposure|
PREPARATION OF WORKING SOLUTION
Add 0.5 mL of β-Mercaptoethanol to 50 mL of ReadiUse™ Viral RNA Lysis Buffer
Note: Store the RNA Lysis Buffer containing β-Mercaptoethanol at 4 °C.
SAMPLE EXPERIMENTAL PROTOCOL
The following protocol can be used as a guideline and should be optimized according to your experimental needs. This protocol was optimized for use with at least 1.5 X 103 cells to a maximum of 5 X 106 cells per purification.
- Wash the cells once with sterile 1X PBS.
- Add 100 µL of ReadiUse™ Viral RNA Lysis Buffer working solution to the washed cells. Mix by pipetting.
- Purify extracted RNA from the previous step using RNA purification method of choice.
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