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ReadiView™ Blue Real-Time PCR Visualization Dye *200X*
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Application notes
ReadiView™ Blue Real-Time PCR Visualization Dye is a 200X concentrated, ready-to-use, inert dye that enhances the visibility of real-time PCR reactions for more accurate pipetting and plate loading. The presence of dye does not adversely affect the performance of the PCR reaction when used as directed and does not interfere with Helixyte™ Green-, SYBR® Green-, or probe-based detection. It is optimized for a wide variety of real-time PCR mixes and kits, including mixes containing ROX and mixes for FAST and regular reaction times.
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
The following protocols serve only as guidelines and should be optimized according to your specific application. Before starting the experiment, the ReadiView™ Blue Real-Time PCR Visualization Dye must be thawed completely to room temperature.
Addition of Dye in the master mix:
- Mix 1 µL of ReadiView™ Blue Real-Time PCR Visualization Dye with 100 µL TAQuest™ qPCR Master Mix (2X master mix) containing primers, TaqMan probes, or DNA dye.
- Mix components thoroughly, then centrifuge briefly to collect the solution at the bottom of the tube. Store on ice, protected from light.
- For a 20 µL PCR reaction, mix 10 µL of qPCR master mix with 10 µL of DNA sample. It is recommended to place the plates on top of a white background to enhance visibility.
Note ReadiView™ Blue Real-Time PCR Visualization Dye may also be compatible with a variety of master mixes but should be validated by the user for optimal performance.
Addition of Dye in the DNA or RNA sample:
- Mix 1 µL of ReadiView™ Blue Real-Time PCR Visualization Dye with 100 µL of samples containing DNA or RNA.
- Mix components thoroughly, then centrifuge briefly to collect the solution at the bottom of the tube. Store on ice, protected from light.
- For a 20 µL PCR reaction, mix 10 µL of qPCR master mix with 10 µL of DNA or RNA sample. It is recommended to place the plates on top of a white background to enhance visibility.
References
View all 50 references: Citation Explorer
SYBR® Green RT-qPCR for the Universal Detection of Citrus Viroids.
Authors: Vidalakis, Georgios and Wang, Jinbo and Dang, Tyler and Rucker, Tavia and Bodaghi, Sohrab and Tan, Shi-Hua and Lavagi-Craddock, Irene and Syed, Alexandra and Uribe, Gerardo and Hsieh, Yi and Carbajal-Moreno, Joana
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 211-217
Authors: Vidalakis, Georgios and Wang, Jinbo and Dang, Tyler and Rucker, Tavia and Bodaghi, Sohrab and Tan, Shi-Hua and Lavagi-Craddock, Irene and Syed, Alexandra and Uribe, Gerardo and Hsieh, Yi and Carbajal-Moreno, Joana
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 211-217
[Typing of Leishmania Species Causing Cutaneous Leishmaniasis by Sybr Green Based ITS-1 Real Time Polymerase Chain Reaction Method].
Authors: Gürses, Gülcan and Yentür Doni, Nebiye and Yıldız Zeyrek, Fadile and Yiğin, Akın
Journal: Mikrobiyoloji bulteni (2022): 326-338
Authors: Gürses, Gülcan and Yentür Doni, Nebiye and Yıldız Zeyrek, Fadile and Yiğin, Akın
Journal: Mikrobiyoloji bulteni (2022): 326-338
Development and validation of multiplex SYBR Green real-time PCR assays for detection and molecular surveillance of four tick-borne canine haemoparasites.
Authors: Padmaja, M and Singh, Harkirat and Panwar, Harsh and Jyoti, and Singh, Niraj Kumar and Singh, Nirbhay Kumar
Journal: Ticks and tick-borne diseases (2022): 101937
Authors: Padmaja, M and Singh, Harkirat and Panwar, Harsh and Jyoti, and Singh, Niraj Kumar and Singh, Nirbhay Kumar
Journal: Ticks and tick-borne diseases (2022): 101937
Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR.
Authors: Tao, Yile and Yue, Yang and Qiu, Guangyu and Ji, Zheng and Spillman, Martin and Gai, Zhibo and Chen, Qingfa and Bielecki, Michel and Huber, Michael and Trkola, Alexandra and Wang, Qiyuan and Cao, Junji and Wang, Jing
Journal: Applied microbiology and biotechnology (2022): 2207-2218
Authors: Tao, Yile and Yue, Yang and Qiu, Guangyu and Ji, Zheng and Spillman, Martin and Gai, Zhibo and Chen, Qingfa and Bielecki, Michel and Huber, Michael and Trkola, Alexandra and Wang, Qiyuan and Cao, Junji and Wang, Jing
Journal: Applied microbiology and biotechnology (2022): 2207-2218
Establishment of SYBR green I-based quantitative real-time polymerase chain reaction for the rapid detection of a novel Chaphamaparvovirus in cats.
Authors: Liu, Xunbi and Li, Shuyan and Liu, Xuan and Wang, Run and Xie, Xiangyu and Wu, Haiqiang and Wang, Yong
Journal: 3 Biotech (2022): 91
Authors: Liu, Xunbi and Li, Shuyan and Liu, Xuan and Wang, Run and Xie, Xiangyu and Wu, Haiqiang and Wang, Yong
Journal: 3 Biotech (2022): 91