ReadiView™ Blue Real-Time PCR Visualization Dye 200X

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Example protocol

SAMPLE EXPERIMENTAL PROTOCOL

The following protocols serve only as guidelines and should be optimized according to your specific application. Before starting the experiment, the ReadiView™ Blue Real-Time PCR Visualization Dye must be thawed completely to room temperature.

Addition of Dye in the master mix:

Mix 1 µL of ReadiView™ Blue Real-Time PCR Visualization Dye with 100 µL TAQuest™ qPCR Master Mix (2X master mix) containing primers, TaqMan probes, or DNA dye.
Mix components thoroughly, then centrifuge briefly to collect the solution at the bottom of the tube. Store on ice, protected from light.
For a 20 µL PCR reaction, mix 10 µL of qPCR master mix with 10 µL of DNA sample. It is recommended to place the plates on top of a white background to enhance visibility.
Note ReadiView™ Blue Real-Time PCR Visualization Dye may also be compatible with a variety of master mixes but should be validated by the user for optimal performance.

Addition of Dye in the DNA or RNA sample:

Mix 1 µL of ReadiView™ Blue Real-Time PCR Visualization Dye with 100 µL of samples containing DNA or RNA.
Mix components thoroughly, then centrifuge briefly to collect the solution at the bottom of the tube. Store on ice, protected from light.
For a 20 µL PCR reaction, mix 10 µL of qPCR master mix with 10 µL of DNA or RNA sample. It is recommended to place the plates on top of a white background to enhance visibility.

Images

Figure 1. Amplification plot for a dilution series of HeLa cells cDNA to detect GAPDH in the absence (green) and presence (blue) of ReadiView™ Blue Real-Time PCR Visualization Dye using TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* (Cat No. 17272).

References

View all 50 references: Citation Explorer

SYBR® Green RT-qPCR for the Universal Detection of Citrus Viroids.
Authors: Vidalakis, Georgios and Wang, Jinbo and Dang, Tyler and Rucker, Tavia and Bodaghi, Sohrab and Tan, Shi-Hua and Lavagi-Craddock, Irene and Syed, Alexandra and Uribe, Gerardo and Hsieh, Yi and Carbajal-Moreno, Joana
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 211-217

[Typing of Leishmania Species Causing Cutaneous Leishmaniasis by Sybr Green Based ITS-1 Real Time Polymerase Chain Reaction Method].
Authors: Gürses, Gülcan and Yentür Doni, Nebiye and Yıldız Zeyrek, Fadile and Yiğin, Akın
Journal: Mikrobiyoloji bulteni (2022): 326-338

Development and validation of multiplex SYBR Green real-time PCR assays for detection and molecular surveillance of four tick-borne canine haemoparasites.
Authors: Padmaja, M and Singh, Harkirat and Panwar, Harsh and Jyoti, and Singh, Niraj Kumar and Singh, Nirbhay Kumar
Journal: Ticks and tick-borne diseases (2022): 101937

Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR.
Authors: Tao, Yile and Yue, Yang and Qiu, Guangyu and Ji, Zheng and Spillman, Martin and Gai, Zhibo and Chen, Qingfa and Bielecki, Michel and Huber, Michael and Trkola, Alexandra and Wang, Qiyuan and Cao, Junji and Wang, Jing
Journal: Applied microbiology and biotechnology (2022): 2207-2218

Establishment of SYBR green I-based quantitative real-time polymerase chain reaction for the rapid detection of a novel Chaphamaparvovirus in cats.
Authors: Liu, Xunbi and Li, Shuyan and Liu, Xuan and Wang, Run and Xie, Xiangyu and Wu, Haiqiang and Wang, Yong
Journal: 3 Biotech (2022): 91

Multiplex SYBR Green real-time PCR for Lactobacillus acidophilus group species targeting biomarker genes revealed by a pangenome approach.
Authors: Kim, Eiseul and Kim, Dayoung and Yang, Seung-Min and Kim, Hae-Yeong
Journal: Microbiological research (2022): 127013

A SYBR Green-based real-time RT-PCR assay to differentiate the H1N1 influenza virus lineages.
Authors: Cong, Yulin and Sun, Yixue and Deng, Xiaoyu and Yu, Haiying and Lian, Xiaohuan and Cong, Yanlong
Journal: Journal of virological methods (2022): 114387

One-step SYBR green-based real-time RT-PCR assay for detection of foot-and-mouth disease virus circulating in India.
Authors: Biswal, Jitendra K and Jena, Biswa Ranjan and Ali, Syed Zeeshan and Ranjan, Rajeev and Mohapatra, Jajati K and Singh, Rabindra Prasad
Journal: Virus genes (2022): 113-121

Development and validation of cost-effective one-step multiplex RT-PCR assay for detecting the SARS-CoV-2 infection using SYBR Green melting curve analysis.
Authors: Sarkar, Shovon Lal and Alam, A S M Rubayet Ul and Das, Prosanto Kumar and Pramanik, Md Hasan Ali and Al-Emran, Hassan M and Jahid, Iqbal Kabir and Hossain, M Anwar
Journal: Scientific reports (2022): 6501

Development and application of SYBR Green Ⅰ real-time quantitative reverse transcription PCR assay for detection of swine Getah virus.
Authors: Xia, Yin-He and Shi, Zi-Cong and Wang, Xin-Wei and Li, Yong-Tao and Wang, Zeng and Chang, Hong-Tao and Liu, Hong-Ying and Chen, Lu and Wang, Chuan-Qing and Yang, Xia
Journal: Molecular and cellular probes (2021): 101730