ReadiView™ Green/Red Viability Stain

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

ReadiView™ Green/Red Viability stain is a robust cell stain optimized for staining nucleated cells in Countess II FL or Countess 3 FL instruments. It can be conveniently used with the traditional FITC or GFP filter sets such as the EVOS GFP light cube are suitable for exciting and detecting the green stain, while RFP or Texas Red (TxR) light cubes are suitable for the red cell stain. Green denotes live cells while red represents dead cells. ReadiView reagent allows for the specific staining of nucleated cells within a mixed population, reducing complexity and improving count-to-count and sample-to-sample repeatability when matched to the appropriate EVOS light cube.

Example protocol

SAMPLE EXPERIMENTAL PROTOCOL

Mix 10 µL of ReadyCount™ Live/Dead Viability stain with 10 µL of cells.
Incubate 2 to 5 minutes at room temperature.
Load 10 µL of stained cell sample into a Countess™ chamber slide.
Count slide on a Countess™ cell counter.

Images

Figure 1. Fluorescence images of Jurkat cells labeled with ReadyView™ Green/Red Viability stain.

References

View all 5 references: Citation Explorer

Drug combinations against Borrelia burgdorferi persisters in vitro: eradication achieved by using daptomycin, cefoperazone and doxycycline.
Authors: Feng, Jie and Auwaerter, Paul G and Zhang, Ying
Journal: PloS one (2015): e0117207

Growth inhibition of cultured marine phytoplankton by toxic algal-derived polyunsaturated aldehydes.
Authors: Ribalet, François and Berges, John A and Ianora, Adrianna and Casotti, Raffaella
Journal: Aquatic toxicology (Amsterdam, Netherlands) (2007): 219-27

An improved method for the selective detection of fungi in hospital waters by solid phase cytometry.
Authors: De Vos, Muriel M and Nelis, Hans J
Journal: Journal of microbiological methods (2006): 557-65

A membrane-immunofluorescent-viability staining technique for the detection of Salmonella spp. from fresh and processed meat samples.
Authors: Duffy, G and Kilbride, B and Sheridan, J J and Blair, I S and McDowell, D A
Journal: Journal of applied microbiology (2000): 587-94

Staining with fluorescein diacetate correlates with hepatocyte function.
Authors: Nyberg, S L and Shatford, R A and Payne, W D and Hu, W S and Cerra, F B
Journal: Biotechnic & histochemistry : official publication of the Biological Stain Commission (1993): 56-63