AAT Bioquest

ReadiView™ Green/Red Viability Stain

Fluorescence images of Jurkat cells labeled with ReadyView™ Green/Red Viability stain.
Fluorescence images of Jurkat cells labeled with ReadyView™ Green/Red Viability stain.
Fluorescence images of Jurkat cells labeled with ReadyView™ Green/Red Viability stain.
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Physical properties
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


ReadiView™ Green/Red Viability stain is a robust cell stain optimized for staining nucleated cells in Countess II FL or Countess 3 FL instruments. It can be conveniently used with the traditional FITC or GFP filter sets such as the EVOS GFP light cube are suitable for exciting and detecting the green stain, while RFP or Texas Red (TxR) light cubes are suitable for the red cell stain. Green denotes live cells while red represents dead cells. ReadiView reagent allows for the specific staining of nucleated cells within a mixed population, reducing complexity and improving count-to-count and sample-to-sample repeatability when matched to the appropriate EVOS light cube.

Example protocol


  1. Mix 10 µL of ReadyCount™ Live/Dead Viability stain with 10 µL of cells.
  2. Incubate 2 to 5 minutes at room temperature.
  3. Load 10 µL of stained cell sample into a Countess™ chamber slide.
  4. Count slide on a Countess™ cell counter. 



View all 5 references: Citation Explorer
Drug combinations against Borrelia burgdorferi persisters in vitro: eradication achieved by using daptomycin, cefoperazone and doxycycline.
Authors: Feng, Jie and Auwaerter, Paul G and Zhang, Ying
Journal: PloS one (2015): e0117207
Growth inhibition of cultured marine phytoplankton by toxic algal-derived polyunsaturated aldehydes.
Authors: Ribalet, François and Berges, John A and Ianora, Adrianna and Casotti, Raffaella
Journal: Aquatic toxicology (Amsterdam, Netherlands) (2007): 219-27
An improved method for the selective detection of fungi in hospital waters by solid phase cytometry.
Authors: De Vos, Muriel M and Nelis, Hans J
Journal: Journal of microbiological methods (2006): 557-65
A membrane-immunofluorescent-viability staining technique for the detection of Salmonella spp. from fresh and processed meat samples.
Authors: Duffy, G and Kilbride, B and Sheridan, J J and Blair, I S and McDowell, D A
Journal: Journal of applied microbiology (2000): 587-94
Staining with fluorescein diacetate correlates with hepatocyte function.
Authors: Nyberg, S L and Shatford, R A and Payne, W D and Hu, W S and Cerra, F B
Journal: Biotechnic & histochemistry : official publication of the Biological Stain Commission (1993): 56-63