Name: Resazurin, sodium salt *CAS 62758-13-8*
Brand: AAT Bioquest
SKU: 15700
Price: 90 USD
Availability: InStock

Resazurin, sodium salt

CAS 62758-13-8

Resazurin is a non-toxic, water-soluble and redox-sensitive dye that changes from its blue/non-fluorescent state to pink/highly-fluorescent upon reduction to resorufin by many biological processes. Resazurin added to viable cells become reduced via an as yet unknown mechanism by enzymatic or chemical reactions in cells. This feature is widely used for measuring cell proliferation, viability and cytotoxicity. Resazurin may be added at any time during the culture period. For measurement of cell proliferation, it is best to add resazurin during log phase growth. Incubation times may vary depending on the metabolic rates of the cell lines being tested. Fluorescence can be read using 544 nm/590 nm filter set. Absorbance can be read using a spectrophotometer set at 570 nm.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
15700	100 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Resazurin, sodium salt *CAS 62758-13-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	398.137 µL	1.991 mL	3.981 mL	19.907 mL	39.814 mL
5 mM	79.627 µL	398.137 µL	796.273 µL	3.981 mL	7.963 mL
10 mM	39.814 µL	199.068 µL	398.137 µL	1.991 mL	3.981 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	251.17 g/mol	=	mL	x	mM	=	mmol

References

View all 65 references: Citation Explorer

Application of the resazurin microtitre assay for detection of multidrug resistance in Mycobacterium tuberculosis in Algiers

Authors:

Nateche F, Martin A, Baraka S, Palomino JC, Khaled S, Portaels F.

Journal:

J Med Microbiol (2006): 857

Evaluation of the resazurin microtiter assay for rapid detection of ofloxacin resistance in M. tuberculosis

Authors:

Umubyeyi AN, Martin A, Zissis G, Struelens M, Karita E, Portaels F.

Journal:

Int J Tuberc Lung Dis (2006): 808

A fluorometric study of relative ocular lens cytosensitivity to multipurpose contact lens solutions using the resazurin assay method

Authors:

Oriowo MO., undefined

Journal:

Toxicol In Vitro (2006): 1548

Development and application of a resazurin-based biomass activity test for activated sludge plant management

Authors:

McNicholl BP, McGrath JW, Quinn JP.

Journal:

Water Res. (2006)

A resazurin-based biosensor for organic pollutants

Authors:

Tizzard AC, Bergsma JH, Lloyd-Jones G.

Journal:

Biosens Bioelectron (2006): 759

Physical properties

Molecular weight	251.17
Solvent	DMSO

Spectral properties

Absorbance (nm)	605
Excitation (nm)	571
Emission (nm)	584

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	62758-13-8

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