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Resazurin, sodium salt *CAS 62758-13-8*

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Physical properties
Molecular weight251.17
Spectral properties
Absorbance (nm)605
Excitation (nm)571
Emission (nm)584
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Absorbance (nm)
Excitation (nm)
Emission (nm)
Resazurin is a non-toxic, water-soluble and redox-sensitive dye that changes from its blue/non-fluorescent state to pink/highly-fluorescent upon reduction to resorufin by many biological processes. Resazurin added to viable cells become reduced via an as yet unknown mechanism by enzymatic or chemical reactions in cells. This feature is widely used for measuring cell proliferation, viability and cytotoxicity. Resazurin may be added at any time during the culture period. For measurement of cell proliferation, it is best to add resazurin during log phase growth. Incubation times may vary depending on the metabolic rates of the cell lines being tested. Fluorescence can be read using 544 nm/590 nm filter set. Absorbance can be read using a spectrophotometer set at 570 nm.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Resazurin, sodium salt *CAS 62758-13-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM398.137 µL1.991 mL3.981 mL19.907 mL39.814 mL
5 mM79.627 µL398.137 µL796.273 µL3.981 mL7.963 mL
10 mM39.814 µL199.068 µL398.137 µL1.991 mL3.981 mL

Molarity calculator

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Spectral properties

Absorbance (nm)605
Excitation (nm)571
Emission (nm)584

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)
Resorufin, sodium salt *CAS 34994-50-8*5715846500010.751-
EDANS sodium salt [5-((2-Aminoethyl)aminonaphthalene-1-sulfonic acid, sodium salt] *CAS 100900-07-0*3364555900-0.107



View all 1 citations: Citation Explorer
Digital Cascade Assays for ADP-or ATP-Producing Enzymes Using a Femtoliter Reactor Array Device
Authors: Ueno, Hiroshi and Sano, Mio and Hara, Mayu and Noji, Hiroyuki
Journal: ACS sensors (2023)


View all 65 references: Citation Explorer
Determination of the diagnostic value of the resazurin reduction assay for evaluating boar semen by receiver operating characteristic analysis
Authors: Zrimsek P, Kosec M, Kunc J, Mrkun J.
Journal: Asian J Androl (2006): 343
Development of resazurin microtiter assay for drug sensibility testing of Trypanosoma cruzi epimastigotes
Authors: Rolon M, Vega C, Escario JA, Gomez-Barrio A.
Journal: Parasitol Res (2006): 103
Rapid susceptibility test for Mycobacterium tuberculosis to isoniazid and rifampin with resazurin method in screw-cap tubes
Authors: Coban AY, Cekic Cihan C, Bilgin K, Uzun M, Akgunes A, Cetinkaya E, Durupinar B.
Journal: J Chemother (2006): 140
A resazurin-based biosensor for organic pollutants
Authors: Tizzard AC, Bergsma JH, Lloyd-Jones G.
Journal: Biosens Bioelectron (2006): 759
Development and application of a resazurin-based biomass activity test for activated sludge plant management
Authors: McNicholl BP, McGrath JW, Quinn JP.
Journal: Water Res. (2006)
A fluorometric study of relative ocular lens cytosensitivity to multipurpose contact lens solutions using the resazurin assay method
Authors: Oriowo MO., undefined
Journal: Toxicol In Vitro (2006): 1548
Application of the resazurin microtitre assay for detection of multidrug resistance in Mycobacterium tuberculosis in Algiers
Authors: Nateche F, Martin A, Baraka S, Palomino JC, Khaled S, Portaels F.
Journal: J Med Microbiol (2006): 857
Evaluation of the resazurin microtiter assay for rapid detection of ofloxacin resistance in M. tuberculosis
Authors: Umubyeyi AN, Martin A, Zissis G, Struelens M, Karita E, Portaels F.
Journal: Int J Tuberc Lung Dis (2006): 808
Comparative evaluation of the nitrate reduction assay, the MTT test, and the resazurin microtitre assay for drug susceptibility testing of clinical isolates of Mycobacterium tuberculosis
Authors: Montoro E, Lemus D, Echemendia M, Martin A, Portaels F, Palomino JC.
Journal: J Antimicrob Chemother (2005): 500
The resazurin microplate method for rapid detection of vancomycin resistance in enterococci
Authors: Coban AY, Darka O, Fisgin NT, Cihan CC, Bilgin K, Akgunes A, Guven T, Dokuzoguz B, Birinci A, Durupinar B.
Journal: J Chemother (2005): 361