RH 414 [N-(3-Triethylammoniumpropyl)-4-(4-(4-(diethylamino)phenyl)butadienyl)pyridinium dibromide] CAS#: 161433-30-3

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Physical properties

Molecular weight	581.47
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

CAS

161433-30-3

Molecular weight

581.47

RH 414 [N-(3-Triethylammoniumpropyl)-4-(4-(4-(diethylamino)phenyl)butadienyl)pyridinium dibromide] is a neuron-tracing dye. It is used for monitoring membrane potential, synaptic activity and ion channel activity of neurons.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of RH 414 [N-(3-Triethylammoniumpropyl)-4-(4-(4-(diethylamino)phenyl)butadienyl)pyridinium dibromide] *CAS#: 161433-30-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	171.978 µL	859.89 µL	1.72 mL	8.599 mL	17.198 mL
5 mM	34.396 µL	171.978 µL	343.956 µL	1.72 mL	3.44 mL
10 mM	17.198 µL	85.989 µL	171.978 µL	859.89 µL	1.72 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Product Family

Name	Excitation (nm)	Emission (nm)
RH 237 [N-(4-Sulfobutyl)-4-(6-(4-(dibutylamino)phenyl)hexatrienyl)pyridinium, inner salt]	550	786
RH 795 CAS#: 172807-13-5	486	689

Images

Figure 1. Chemical structure for RH 414 [N-(3-Triethylammoniumpropyl)-4-(4-(4-(diethylamino)phenyl)butadienyl)pyridinium dibromide] *CAS#: 161433-30-3*

Citations

View all 1 citations: Citation Explorer

Streamlining of a synthetic co-culture towards an individually controllable one-pot process for polyhydroxyalkanoate production from light and CO2
Authors: Kratzl, Franziska and Kremling, Andreas and Pfl{\"u}ger-Grau, Katharina
Journal: Engineering in Life Sciences (2022)

References

View all 37 references: Citation Explorer

Regionalization of plasma membrane-bound flavoproteins of cerebellar granule neurons in culture by fluorescence energy transfer imaging
Authors: Samhan-Arias AK, Garcia-Bereguiain MA, Martin-Romero FJ, Gutierrez-Merino C.
Journal: J Fluoresc (2006): 393

Endogenous acetylcholine enhances synchronized interneuron activity in rat neocortex
Authors: B, undefined and yopadhyay S, Sutor B, Hablitz JJ.
Journal: J Neurophysiol (2006): 1908

Horizontal spread of activity in neocortical inhibitory networks
Authors: DeFazio RA, Hablitz JJ.
Journal: Brain Res Dev Brain Res (2005): 83

Photostability of a fluorescent marker under pulsed excited-state depletion through stimulated emission
Authors: Dyba M, Hell SW.
Journal: Appl Opt (2003): 5123

Optical analysis of acute spontaneous epileptiform discharges in the in vivo rat cerebral cortex
Authors: Miyakawa N, Yazawa I, Sasaki S, Momose-Sato Y, Sato K.
Journal: Neuroimage (2003): 622

Visualization of modulatory effects of serotonin in the silkmoth antennal lobe
Authors: Hill ES, Okada K, Kanzaki R.
Journal: J Exp Biol (2003): 345

Electrotonically mediated oscillatory patterns in neuronal ensembles: an in vitro voltage-dependent dye-imaging study in the inferior olive
Authors: Leznik E, Makarenko V, Llinas R.
Journal: J Neurosci (2002): 2804

Interactions between multiple rhythm generators produce complex patterns of oscillation in the developing rat spinal cord
Authors: Demir R, Gao BX, Jackson MB, Ziskind-Conhaim L.
Journal: J Neurophysiol (2002): 1094

Functional independence of layer IV barrels
Authors: Laaris N, Keller A.
Journal: J Neurophysiol (2002): 1028

Generation and propagation of subthreshold waves in a network of inferior olivary neurons
Authors: Devor A, Yarom Y.
Journal: J Neurophysiol (2002): 3059