Rhod-2 AM | AAT Bioquest

Rhod-2, AM

UltraPure Grade; CAS#: 145037-81-6

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. The long-wavelength Rhod-2 Ca2+ indicators are valuable alternatives to Fluo-3 for experiments in cells and tissues that have high levels of autofluorescence. Rhod-2 AM is cell-permeable version of Rhod-2.

Protocol

COO

COA

Catalog	Size	Price
21062	1 mg	Price
21063	50 mg	Price
21064	20x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhod-2, AM *UltraPure Grade* *CAS#: 145037-81-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	88.971 µL	444.856 µL	889.711 µL	4.449 mL	8.897 mL
5 mM	17.794 µL	88.971 µL	177.942 µL	889.711 µL	1.779 mL
10 mM	8.897 µL	44.486 µL	88.971 µL	444.856 µL	889.711 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1123.96 g/mol	=	mL	x	mM	=	mmol

Citations

View all 25 citations: Citation Explorer

Evidence for the cardiodepressive effects of the plasticizer di-2-ethylhexylphthalate (DEHP)

Authors:

Swift, Luther M and Roberts, Anysja and Pressman, Jenna and Guerrelli, Devon and Allen, Samuel and Haq, Kazi T and Reisz, Julie A and D’Alessandro, Angelo and Posnack, Nikki Gillum

Journal:

bioRxiv (2023): 2023--05

Alterations in Mitochondria-Associated Endoplasmic Reticulum Membranes Under Oxidative Stress in R28 Cells

Authors:

Yang, Yuting and Wu, Jihong and Lu, Wei and Dai, Yiqin and Zhang, Youjia and Sun, Xinghuai

Dynamic changes in $\beta$-cell [Ca2+] regulate NFAT activation, gene transcription, and islet gap junction communication

Authors:

Miranda, Jose G and Schleicher, Wolfgang E and Wells, Kristen L and Ramirez, David G and Landgrave, Samantha P and Benninger, Richard KP

Journal:

Molecular metabolism (2022): 101430

Metabolic rescue ameliorates mitochondrial encephalo-cardiomyopathy in murine and human iPSC models of Leigh syndrome

Authors:

Yoon, Jin-Young and Daneshgar, Nastaran and Chu, Yi and Chen, Biyi and Hefti, Marco and Irani, Kaikobad and Song, Long-Sheng and Brenner, Charles and Abel, E Dale and London, Barry and others,

Journal:

bioRxiv (2022)

Dynamic changes in $\beta$-cell electrical activity and [Ca2+] regulates NFATc3 activation and downstream gene transcription

Authors:

Miranda, Jose G and Schleicher, Wolfgang E and Ramirez, David G and Landgrave, Samantha P and Benninger, Richard KP

Journal:

BioRXiv (2020)

Physical properties

Dissociation constant (K_d, nM)	570
Molecular weight	1123.96
Solvent	DMSO

Spectral properties

Excitation (nm)	553
Emission (nm)	577
Quantum yield	0.1 ¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	145037-81-6

Instrument settings

Fluorescence microscope
Excitation	TRITC filter set
Emission	TRITC filter set
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	540
Emission	590
Cutoff	570
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

Documents

Protocol
Safety Data Sheet (Catalog 21062)
Safety Data Sheet (Catalog 21063)
Safety Data Sheet (Catalog 21064)
Certificate of Origin
Certificate of Analysis

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Page updated on September 20, 2024