Rhod-2 dextran conjugate *Low affinity with MW 10,000*

Rhod-2 dextran conjugate

Low affinity with MW 10,000

Dextran-conjugated calcium indicators are stably retained within neurons. As a result, they are well suited to measuring presynaptic calcium at physiological temperatures. In addition, dextran indicators can be used to label neurons and their presynaptic boutons in vivo. This has allowed measurements of calcium in the presynaptic boutons of projection fibers that cannot be stably loaded with other types of indicators. Stephan D. Brenowitz and Wade G. Regehr reported a technique for in vivo loading of the climbing fiber projection to the cerebellum with Rhod 2-dextran indicators for presynaptic calcium imaging in brain slices. This technique is applicable to studies of projection fibers in many species from which brain slices can be prepared. The dextran indicators can be injected into the inferior olive using a stereotaxic device. This Rhod 2-dextran conjugate has low affinity with Kd = 3.8 uM.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20451	1 mg	Price

Citations

View all 3 citations: Citation Explorer

Altered expression of Presenilin2 impacts endolysosomal homeostasis and synapse function in Alzheimer’s disease-relevant brain circuits

Authors:

Perdok, Anika and Van Acker, Zo{\"e} P and Vrancx, C{\'e}line and Sannerud, Ragna and Vorsters, Inge and Verrengia, Assunta and Callaerts-V{\'e}gh, Zsuzsanna and Creemers, Eline and Guti{\'e}rrez Fern{\'a}ndez, Sara and D’hauw, Britt and others,

Journal:

Nature Communications (2024): 1--20

A Lipid-Based Droplet Processor for Parallel Chemical Signals

Authors:

Cazimoglu, Idil and Booth, Michael J and Bayley, Hagan

Journal:

ACS nano (2021): 20214--20224

Tuning the Color Palette of Fluorescent Copper Sensors through Systematic Heteroatom Substitution at Rhodol Cores

Authors:

Jia, Shang and Ramos-Torres, Karla M and Kolemen, Safacan and Ackerman, Cheri M and Chang, Christopher J

References

View all 11 references: Citation Explorer

Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1

Authors:

Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.

Journal:

Methods Mol Biol (2011): 377

An ensemble and single-molecule fluorescence spectroscopy investigation of Calcium Green 1, a calcium-ion sensor

Authors:

Lu Y, Paige MF.

Journal:

J Fluoresc (2007): 739

Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator

Authors:

Tour O, Adams SR, Kerr RA, Meijer RM, Sejnowski TJ, Tsien RW, Tsien RY.

Journal:

Nat Chem Biol (2007): 423

Measurement of intracellular calcium levels by the fluorescent Ca2+ indicator Calcium-Green

Authors:

Silei V, Fabrizi C, Venturini G, Tagliavini F, Salmona M, Bugiani O, Lauro GM.

Journal:

Brain Res Brain Res Protoc (2000): 132

Optical imaging of neuronal activity in tissue labeled by retrograde transport of Calcium Green Dextran

Authors:

McPherson DR, McClellan AD, O'Donovan MJ.

Journal:

Brain Res Brain Res Protoc (1997): 157

Physical properties

Molecular weight	~11000
Solvent	Water

Spectral properties

Excitation (nm)	553
Emission (nm)	577
Quantum yield	0.1 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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