Rhod-2 dextran conjugate *Low affinity with MW 10,000*
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
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Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | ~11000 |
Solvent | Water |
Spectral properties
Excitation (nm) | 553 |
Emission (nm) | 577 |
Quantum yield | 0.11 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Alternative formats
Rhod-2 dextran conjugate *Low affinity with MW 3000* |
Overview | SDSProtocol |
Molecular weight ~11000 | Excitation (nm) 553 | Emission (nm) 577 | Quantum yield 0.11 |
Dextran-conjugated calcium indicators are stably retained within neurons. As a result, they are well suited to measuring presynaptic calcium at physiological temperatures. In addition, dextran indicators can be used to label neurons and their presynaptic boutons in vivo. This has allowed measurements of calcium in the presynaptic boutons of projection fibers that cannot be stably loaded with other types of indicators. Stephan D. Brenowitz and Wade G. Regehr reported a technique for in vivo loading of the climbing fiber projection to the cerebellum with Rhod 2-dextran indicators for presynaptic calcium imaging in brain slices. This technique is applicable to studies of projection fibers in many species from which brain slices can be prepared. The dextran indicators can be injected into the inferior olive using a stereotaxic device. This Rhod 2-dextran conjugate has low affinity with Kd = 3.8 uM.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Excitation (nm) | 553 |
Emission (nm) | 577 |
Quantum yield | 0.11 |
Citations
View all 2 citations: Citation Explorer
A Lipid-Based Droplet Processor for Parallel Chemical Signals
Authors: Cazimoglu, Idil and Booth, Michael J and Bayley, Hagan
Journal: ACS nano (2021): 20214--20224
Authors: Cazimoglu, Idil and Booth, Michael J and Bayley, Hagan
Journal: ACS nano (2021): 20214--20224
Tuning the Color Palette of Fluorescent Copper Sensors through Systematic Heteroatom Substitution at Rhodol Cores
Authors: Jia, Shang and Ramos-Torres, Karla M and Kolemen, Safacan and Ackerman, Cheri M and Chang, Christopher J
Journal: (2017)
Authors: Jia, Shang and Ramos-Torres, Karla M and Kolemen, Safacan and Ackerman, Cheri M and Chang, Christopher J
Journal: (2017)
References
View all 11 references: Citation Explorer
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Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377
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Journal: Nat Chem Biol (2007): 423
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Journal: Nat Chem Biol (2007): 423
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Journal: J Fluoresc (2007): 739
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Journal: Brain Res Brain Res Protoc (2000): 132
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Journal: Biochem Biophys Res Commun (1997): 189
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Journal: Brain Res Brain Res Protoc (1997): 157
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Journal: Plant Physiol (1996): 913
Authors: Askerlund P., undefined
Journal: Plant Physiol (1996): 913
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Journal: Pflugers Arch (1995): 579
Authors: Spencer CI, Berlin JR.
Journal: Pflugers Arch (1995): 579
Characterization of calcium translocation across the plasma membrane of primary osteoblasts using a lipophilic calcium-sensitive fluorescent dye, calcium green C18
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Authors: Lloyd QP, Kuhn MA, Gay CV.
Journal: J Biol Chem (1995): 22445
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Journal: Neurosci Lett (1993): 149
Authors: Rajdev S, Reynolds IJ.
Journal: Neurosci Lett (1993): 149
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