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Rhod-4™, sodium salt

ATP-stimulated calcium responses of endogenous P2Y receptors were measured in CHO-K1 cells with Rhod-4™ AM (Cat# 21120) and Rhod-2 AM (Cat# 21064). CHO-K1 cells were seeded overnight at 50,000 cells/100 µL/well in a Costar 96-well black wall/clear bottom plate. The growth medium was removed, and the cells were incubated with 100 µL of dye loading solution using Rhod-4™ AM (4 µM, A and B) or Rhod-2 AM (4 µM, C and D) for 1 hour in a 37 °C, 5% CO2 incubator. The staining solution was replaced with 200 µL HHBS, then the cells were imaged before (A and C) and after (B and D) ATP treatment with a fluorescence microscope (Olympus IX71) using TRITC channel.
ATP-stimulated calcium responses of endogenous P2Y receptors were measured in CHO-K1 cells with Rhod-4™ AM (Cat# 21120) and Rhod-2 AM (Cat# 21064). CHO-K1 cells were seeded overnight at 50,000 cells/100 µL/well in a Costar 96-well black wall/clear bottom plate. The growth medium was removed, and the cells were incubated with 100 µL of dye loading solution using Rhod-4™ AM (4 µM, A and B) or Rhod-2 AM (4 µM, C and D) for 1 hour in a 37 °C, 5% CO2 incubator. The staining solution was replaced with 200 µL HHBS, then the cells were imaged before (A and C) and after (B and D) ATP treatment with a fluorescence microscope (Olympus IX71) using TRITC channel.
Ordering information
Price ()
Catalog Number21118
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Dissociation constant (Kd, nM)451
Molecular weight815.64
SolventWater
Spectral properties
Excitation (nm)523
Emission (nm)551
Quantum yield0.11
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
815.64
Dissociation constant (Kd, nM)
451
Excitation (nm)
523
Emission (nm)
551
Quantum yield
0.11
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-2 is most commonly used among the red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Rhod-4™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2. In CHO and HEK cells Rhod-4™ AM has cellular calcium response that is 10 times more sensitive than Rhod-2 AM. AAT Bioquest offers versatile packing sizes of Quest Rhod-4 to meet your special needs, e.g., 1 mg; 10x50 µg; 20x50 µg; HTS packages with no additional packaging charge.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Rhod-4™, sodium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM122.603 µL613.016 µL1.226 mL6.13 mL12.26 mL
5 mM24.521 µL122.603 µL245.206 µL1.226 mL2.452 mL
10 mM12.26 µL61.302 µL122.603 µL613.016 µL1.226 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Excitation (nm)523
Emission (nm)551
Quantum yield0.11

Product family


NameExcitation (nm)Emission (nm)Quantum yield
Rhod-4™, potassium salt5235510.11
Rhod-4™, potassium salt5235510.11

Citations


View all 23 citations: Citation Explorer
Emerin plays a crucial role in nuclear invagination and in the nuclear calcium transient
Authors: Shimojima, Masaya and Yuasa, Shinsuke and Motoda, Chikaaki and Yozu, Gakuto and Nagai, Toshihiro and Ito, Shogo and Lachmann, Mark and Kashimura, Shin and Takei, Makoto and Kusumoto, Dai and others, undefined
Journal: Scientific Reports (2017)
Preliminary findings on ultrasound modulation of the electromechanical function of human stem-cell-derived cardiomyocytes
Authors: Chen, Andrew William and Klimas, Aleks and ra , undefined and Zderic, Vesna and Castellanos, Ivan Suares and Entcheva, Emilia
Journal: (2017): 1--4
The role of spatial organization of Ca (2+) release sites in the generation of arrhythmogenic diastolic Ca (2+) release in myocytes from failing hearts.
Authors: Belevych, Andriy E and Ho, Hsiang-Ting and Bonilla, Ingrid M and Terentyeva, Radmila and Schober, Karsten E and Terentyev, Dmitry and Carnes, Cynthia A and Györke, Sándor
Journal: Basic research in cardiology (2017): 44
Individual evaluation of cardiac marker expression and self-beating during cardiac differentiation of P19CL6 cells on different culture substrates
Authors: Yamaoka, Tetsuji and Hirata, Mitsuhi and Dan, Takaaki and Yamashita, Atsushi and Otaka, Akihisa and Nakaoki, Takahiko and Miskon, Azizi and Kakinoki, Sachiro and Mahara, Atsushi
Journal: Journal of Biomedical Materials Research Part A (2016)
Multiple pathways for elevating extracellular adenosine in the rat hippocampal CA1 region characterized by adenosine sensor cells
Authors: Yamashiro, Kunihiko and Fujii, Yuki and Maekawa, Shohei and Morita, Mitsuhiro
Journal: Journal of Neurochemistry (2016)
The G protein-coupled receptor GPR157 regulates neuronal differentiation of radial glial progenitors through the Gq-IP3 pathway
Authors: Takeo, Yutaka and Kurabayashi, Nobuhiro and Nguyen, Minh Dang and Sanada, Kamon
Journal: Scientific reports (2016)
Dynamic polyrotaxane-coated surface for effective differentiation of mouse induced pluripotent stem cells into cardiomyocytes
Authors: Seo, Ji-Hun and Hirata, Mitsuhi and Kakinoki, Sachiro and Yamaoka, Tetsuji and Yui, Nobuhiko
Journal: RSC Advances (2016): 35668--35676
Involvement of aberrant calcium signalling in herpetic neuralgia
Authors: Warwick, Rebekah A and Hanani, Menachem
Journal: Experimental neurology (2016): 10--18
OptoDyCE as an automated system for high-throughput all-optical dynamic cardiac electrophysiology
Authors: Klimas, Aleks and ra , undefined and Ambrosi, Christina M and Yu, Jinzhu and Williams, John C and Bien, Harold and Entcheva, Emilia
Journal: Nature communications (2016)