Rhod-5N, tripotassium salt

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-5N has a lower binding affinity for Ca2+ (Kd = ~320 µM) than any other BAPTA-based indicator and is suitable for Ca2+ measurements from 10 µM to 1 mM. Like the parent Rhod-2 indicator, Rhod-5N is essentially nonfluorescent in the absence of divalent cations and exhibits strong fluorescence enhancement with no spectral shift upon binding Ca2+.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21072	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Rhod-5N, tripotassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	111.109 µL	555.543 µL	1.111 mL	5.555 mL	11.111 mL
5 mM	22.222 µL	111.109 µL	222.217 µL	1.111 mL	2.222 mL
10 mM	11.111 µL	55.554 µL	111.109 µL	555.543 µL	1.111 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	900.02 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Imaging Ca2+ Concentration and pH in Nanopores/Channels of Protein Crystals

Authors:

Mori, Kazuo and Kuhn, Bernd

Journal:

The Journal of Physical Chemistry B (2018): 9646--9653

Activation of mitochondrial transient receptor potential vanilloid 1 channel contributes to microglial migration

Authors:

Miyake, Takahito and Shirakawa, Hisashi and Nakagawa, Takayuki and Kaneko, Shuji

Journal:

Glia (2015): 1870--1882

ER stress in human hepatic cells treated with Efavirenz: mitochondria again

Authors:

Apostolova, Nadezda and Gomez-Sucerquia, Leysa J and Alegre, Fern and o , undefined and Funes, Haryes A and Victor, Victor M and Barrachina, Maria D and Blas-Garcia, Ana and Esplugues, Juan V

Journal:

Journal of hepatology (2013): 780--789

Physical properties

Dissociation constant (K_d, nM)	300000
Molecular weight	900.02
Solvent	Water

Spectral properties

Excitation (nm)	557
Emission (nm)	580

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 13, 2025