Rhodamine 101 Inner Salt CAS 116450-56-7

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Physical properties

Molecular weight	490.59
Solvent	DMSO

Spectral properties

Excitation (nm)	569
Emission (nm)	590
Quantum yield	0.1¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

116450-56-7

Molecular weight

490.59

Excitation (nm)

569

Emission (nm)

590

Quantum yield

0.1¹

Rhodamine dyes are used extensively in a variety of biological applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. Rhodamine 101 is one of the brightest fluorescent dyes. This rhodamine dye is often used a reference standard for 5-ROX or 6-ROX labeled bioconjugates.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine 101 Inner Salt *CAS 116450-56-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	203.836 µL	1.019 mL	2.038 mL	10.192 mL	20.384 mL
5 mM	40.767 µL	203.836 µL	407.672 µL	2.038 mL	4.077 mL
10 mM	20.384 µL	101.918 µL	203.836 µL	1.019 mL	2.038 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	569
Emission (nm)	590
Quantum yield	0.1¹

Product Family

Name	Excitation (nm)	Emission (nm)	Quantum yield
Rhodamine 101 Chloride Salt CAS 64339-18-0	569	590	0.1¹

Images

Figure 1. Chemical structure for Rhodamine 101 Inner Salt *CAS 116450-56-7*

References

View all 32 references: Citation Explorer

Coherently-controlled two-dimensional photon echo electronic spectroscopy
Authors: Prokhorenko VI, Halpin A, Miller RJ.
Journal: Opt Express (2009): 9764

Lasing with well-defined cavity modes in dye-infiltrated silica inverse opals
Authors: Nishijima Y, Ueno K, Juodkazis S, Mizeikis V, Fujiwara H, Sasaki K, Misawa H.
Journal: Opt Express (2009): 2976

Validation of fluorescence quantum yields for light-scattering powdered samples by laser-induced optoacoustic spectroscopy
Authors: Tomasini EP, San Roman E, Braslavsky SE.
Journal: Langmuir (2009): 5861

Accurate determination of the limiting anisotropy of rhodamine 101. Implications for its use as a fluorescence polarization standard
Authors: Prazeres TJ, Fedorov A, Barbosa SP, Martinho JM, Berberan-Santos MN.
Journal: J Phys Chem A (2008): 5034

A mesoporous silica thin film as uptake host for guest molecules with retarded release kinetics
Authors: Suh M, Lee HJ, Park JY, Lee UH, Kwon YU, Kim DJ.
Journal: Chemphyschem (2008): 1402

Energy transfer from chemically attached rhodamine 101 to adsorbed methylene blue on microcrystalline cellulose particles
Authors: Rodriguez HB, Roman ES.
Journal: Photochem Photobiol (2007): 547

Mechanically tunable optofluidic distributed feedback dye laser
Authors: Li Z, Zhang Z, Scherer A, Psaltis D.
Journal: Opt Express (2006): 10494

Role of oxidative stress in the apoptosis of hepatocellular carcinoma induced by combination of arsenic trioxide and ascorbic acid
Authors: Li JJ, Tang Q, Li Y, Hu BR, Ming ZY, Fu Q, Qian JQ, Xiang JZ.
Journal: Acta Pharmacol Sin (2006): 1078

Interfacial behavior of sulforhodamine 101 at the polarized water/1,2-dichloroethane interface studied by spectroelectrochemical techniques
Authors: Nagatani H, Suzuki S, Fermin DJ, Girault HH, Nakatani K.
Journal: Anal Bioanal Chem (2006): 633

Comparison of tracer techniques for monitoring sewer losses
Authors: Revitt DM, Ellis JB, Paterakis N.
Journal: J Environ Monit (2006): 564