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Rhodamine 101 Inner Salt *CAS 116450-56-7*

Rhodamine dyes are used extensively in a variety of biological applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. Rhodamine 101 is one of the brightest fluorescent dyes. This rhodamine dye is often used a reference standard for 5-ROX or 6-ROX labeled bioconjugates.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine 101 Inner Salt *CAS 116450-56-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM203.836 µL1.019 mL2.038 mL10.192 mL20.384 mL
5 mM40.767 µL203.836 µL407.672 µL2.038 mL4.077 mL
10 mM20.384 µL101.918 µL203.836 µL1.019 mL2.038 mL

Molarity calculator

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Spectrum

Product family

NameExcitation (nm)Emission (nm)Quantum yield
Rhodamine 101 Chloride Salt *CAS 64339-18-0*5695900.11

References

View all 32 references: Citation Explorer
Coherently-controlled two-dimensional photon echo electronic spectroscopy
Authors: Prokhorenko VI, Halpin A, Miller RJ.
Journal: Opt Express (2009): 9764
Lasing with well-defined cavity modes in dye-infiltrated silica inverse opals
Authors: Nishijima Y, Ueno K, Juodkazis S, Mizeikis V, Fujiwara H, Sasaki K, Misawa H.
Journal: Opt Express (2009): 2976
Validation of fluorescence quantum yields for light-scattering powdered samples by laser-induced optoacoustic spectroscopy
Authors: Tomasini EP, San Roman E, Braslavsky SE.
Journal: Langmuir (2009): 5861
Accurate determination of the limiting anisotropy of rhodamine 101. Implications for its use as a fluorescence polarization standard
Authors: Prazeres TJ, Fedorov A, Barbosa SP, Martinho JM, Berberan-Santos MN.
Journal: J Phys Chem A (2008): 5034
A mesoporous silica thin film as uptake host for guest molecules with retarded release kinetics
Authors: Suh M, Lee HJ, Park JY, Lee UH, Kwon YU, Kim DJ.
Journal: Chemphyschem (2008): 1402
Page updated on October 12, 2024

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Physical properties

Molecular weight

490.59

Solvent

DMSO

Spectral properties

Excitation (nm)

569

Emission (nm)

590

Quantum yield

0.11

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

116450-56-7
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