ROS Brite™ 570 *Optimized for Detecting Reactive Oxygen Species (ROS)*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 732.81 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 555 |
Emission (nm) | 568 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Reactive Oxygen Species (ROS)
Molecular weight 732.81 | Excitation (nm) 555 | Emission (nm) 568 |
Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen. Examples include superoxide, hydroxyl radical, singlet oxygen and peroxides. ROS is highly reactive due to the presence of unpaired valence shell electrons. ROS forms as a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis. However, during times of environmental stress (e.g., UV or heat exposure), ROS levels can increase dramatically. This may result in significant damage to cell structures. Cumulatively, this is known as oxidative stress. ROS are also generated by exogenous sources such as ionizing radiation. Under conditions of oxidative stress, ROS production is dramatically increased, resulting in subsequent alteration of membrane lipids, proteins, and nucleic acids. Oxidative damage of these biomolecules is associated with aging as well as with a variety of pathological events, including atherosclerosis, carcinogenesis, ischemic reperfusion injury, and neurodegenerative disorders. ROS Brite™ 570 reagent is a new fluorogenic probe to measure oxidative stress in cells using conventional fluorescence microscopy, high-content imaging, microplate fluorometry, or flow cytometry. The cell-permeant ROS Brite™ 570 reagent is nonfluorescent and produces bright orange fluorescence upon ROS oxidation. The resulting fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry. It is an excellent alternative to CellROX™ Orange Reagent (C10443) for oxidative stress detection (CellROX™ is a trademark of ThermoFisher).
Platform
Fluorescence microscope
Excitation | Cy3/TRITC filter set |
Emission | Cy3/TRITC filter set |
Recommended plate | Black wall/clear bottom |
Instrument specification(s) | Cy3/TRITC filter set |
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ROS Brite™ 570 *Optimized for Detecting Reactive Oxygen Species (ROS)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 136.461 µL | 682.305 µL | 1.365 mL | 6.823 mL | 13.646 mL |
5 mM | 27.292 µL | 136.461 µL | 272.922 µL | 1.365 mL | 2.729 mL |
10 mM | 13.646 µL | 68.231 µL | 136.461 µL | 682.305 µL | 1.365 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Images
Figure 1. Fluorescence response of ROS Brite™ 570 to different reactive oxygen species in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 540/590 nm.
Figure 2. The ferroptosis inhibitors (DFO, Ferro-1), the ROS scavengers (Trolox, Tempol), the apoptosis inhibitor (Z-VAD), the necroptosis inhibitor (Nec-1), or the autophagy inhibitor (CQ) cannot rescue TXNRD1 inhibitor-induced cell death in pre-OCs. ROS levels were detected by ROS Brite 570 using flow cytometry. Top row: BMDMs and pre-OCs was measured after 24 h of incubation with a medium containing 5 μM TRi-1 with or without 2 μM Ferrostantin-1 or 50 μM DFO. Bottom row: BMDMs and pre-OCs was measured after 5 h of incubation with a medium containing 2 μM AF or 5 μM TRi-1with or without 100 μM Trolox or 25 μM Tempol. n = 3 per group. All data in this figure are represented as mean ± SD. ns, no significance, *P < 0.01, **P < 0.01, ***P < 0.001, ****P < 0.0001. Source: NFATc1-mediated expression of SLC7A11 drives sensitivity to TXNRD1 inhibitors in osteoclast precursors by Zhong, et.al., Redox Biology, April 2023.
Citations
View all 10 citations: Citation Explorer
NFATc1-mediated expression of SLC7A11 drives sensitivity to TXNRD1 inhibitors in osteoclast precursors
Authors: Zhong, Zeyuan and Zhang, Chongjing and Ni, Shuo and Ma, Miao and Zhang, Xiaomeng and Sang, Weicong and Lv, Tao and Qian, Zhi and Yi, Chengqing and Yu, Baoqing
Journal: Redox Biology (2023): 102711
Authors: Zhong, Zeyuan and Zhang, Chongjing and Ni, Shuo and Ma, Miao and Zhang, Xiaomeng and Sang, Weicong and Lv, Tao and Qian, Zhi and Yi, Chengqing and Yu, Baoqing
Journal: Redox Biology (2023): 102711
Low level of antioxidant capacity biomarkers but not target overexpression predicts vulnerability to ROS-inducing drugs
Authors: Samarin, Jana and Fabrowski, Piotr and Kurilov, Roman and Nuskova, Hana and Hummel-Eisenbeiss, Johanna and Pink, Hannelore and Li, Nan and Weru, Vivienn and Alborzinia, Hamed and Yildiz, Umut and others,
Journal: bioRxiv (2023): 2023--01
Authors: Samarin, Jana and Fabrowski, Piotr and Kurilov, Roman and Nuskova, Hana and Hummel-Eisenbeiss, Johanna and Pink, Hannelore and Li, Nan and Weru, Vivienn and Alborzinia, Hamed and Yildiz, Umut and others,
Journal: bioRxiv (2023): 2023--01
Involvement of a flavoprotein, acetohydroxyacid synthase, in growth and riboflavin production in riboflavin-overproducing Ashbya gossypii mutant
Authors: Kato, Tatsuya and Kano, Mai and Yokomori, Ami and Azegami, Junya and El Enshasy, Hesham A and Park, Enoch Y
Journal: Microbial Cell Factories (2023): 1--10
Authors: Kato, Tatsuya and Kano, Mai and Yokomori, Ami and Azegami, Junya and El Enshasy, Hesham A and Park, Enoch Y
Journal: Microbial Cell Factories (2023): 1--10
Toxin-Enabled “On-Demand” Liposomes for Enhanced Phototherapy to Treat and Protect against Methicillin-Resistant Staphylococcus aureus Infection
Authors: Zhuge, Deli and Chen, Mengchun and Yang, Xuewei and Zhang, Xufei and Yao, Lulu and Li, Li and Wang, Haonan and Chen, Hao and Yin, Qingqing and Tian, Dongyan and others,
Journal: Small (2022): 2203292
Authors: Zhuge, Deli and Chen, Mengchun and Yang, Xuewei and Zhang, Xufei and Yao, Lulu and Li, Li and Wang, Haonan and Chen, Hao and Yin, Qingqing and Tian, Dongyan and others,
Journal: Small (2022): 2203292
Mechanisms of Nanoparticle Toxicity in Cancer and Normal Cells
Authors: Tzelepi, Konstantina Nadia
Journal: (2019)
Authors: Tzelepi, Konstantina Nadia
Journal: (2019)
Thiol-Mediated Synthesis of Hyaluronic Acid-Epigallocatechin-3-O-Gallate Conjugates for the Formation of Injectable Hydrogels with Free Radical Scavenging Property and Degradation Resistance
Authors: Liu, Chixuan and Bae, Ki Hyun and Yamashita, Atsushi and Chung, Joo Eun and Kurisawa, Motoichi
Journal: Biomacromolecules (2017)
Authors: Liu, Chixuan and Bae, Ki Hyun and Yamashita, Atsushi and Chung, Joo Eun and Kurisawa, Motoichi
Journal: Biomacromolecules (2017)
Thiol-mediated synthesis of hyaluronic acid--epigallocatechin-3-O-gallate conjugates for the formation of injectable hydrogels with free radical scavenging property and degradation resistance
Authors: Liu, Chixuan and Bae, Ki Hyun and Yamashita, Atsushi and Chung, Joo Eun and Kurisawa, Motoichi
Journal: Biomacromolecules (2017): 3143--3155
Authors: Liu, Chixuan and Bae, Ki Hyun and Yamashita, Atsushi and Chung, Joo Eun and Kurisawa, Motoichi
Journal: Biomacromolecules (2017): 3143--3155
Transient receptor potential melastatin 8 ion channel in macrophages modulates colitis through a balance-shift in TNF-alpha and interleukin-10 production
Authors: Khalil, M and Babes, A and Lakra, R and Försch, S and Reeh, PW and Wirtz, S and Becker, C and Neurath, MF and Engel, MA
Journal: Mucosal immunology (2016)
Authors: Khalil, M and Babes, A and Lakra, R and Försch, S and Reeh, PW and Wirtz, S and Becker, C and Neurath, MF and Engel, MA
Journal: Mucosal immunology (2016)
VEGFR2 signaling prevents colorectal cancer cell senescence to promote tumorigenesis in mice with colitis
Authors: Foersch, Sebastian and Sperka, Tobias and Lindner, Christina and Taut, Astrid and Rudolph, Karl L and Breier, Georg and Boxberger, Frank and Rau, Tilman T and Hartmann, Arndt and Stürzl, Michael and others, undefined
Journal: Gastroenterology (2015): 177--189
Authors: Foersch, Sebastian and Sperka, Tobias and Lindner, Christina and Taut, Astrid and Rudolph, Karl L and Breier, Georg and Boxberger, Frank and Rau, Tilman T and Hartmann, Arndt and Stürzl, Michael and others, undefined
Journal: Gastroenterology (2015): 177--189
Oxidative Stress--An Update and Insight in the Romanian Family Physician’s Adoption of the Concept
Authors: Berghea, Florian and Berghea, Camelia Elena and Abobului, Mihai
Journal: Internal Medicine : 11--15
Authors: Berghea, Florian and Berghea, Camelia Elena and Abobului, Mihai
Journal: Internal Medicine : 11--15
References
View all 91 references: Citation Explorer
Lipoxin A inhibits porphyromonas gingivalis-induced aggregation and reactive oxygen species production by modulating neutrophil-platelet interaction and CD11b expression
Authors: Borgeson E, Lonn J, Bergstrom I, Brodin VP, Ramstrom S, Nayeri F, Sarndahl E, Bengtsson T.
Journal: Infect Immun (2011): 1489
Authors: Borgeson E, Lonn J, Bergstrom I, Brodin VP, Ramstrom S, Nayeri F, Sarndahl E, Bengtsson T.
Journal: Infect Immun (2011): 1489
Role of hyaluronan and CD44 in reactive oxygen species-induced mucus hypersecretion
Authors: Yu H, Li Q, Zhou X, Kolosov VP, Perelman JM.
Journal: Mol Cell Biochem (2011): 65
Authors: Yu H, Li Q, Zhou X, Kolosov VP, Perelman JM.
Journal: Mol Cell Biochem (2011): 65
Nickel nanowires induced and reactive oxygen species mediated apoptosis in human pancreatic adenocarcinoma cells
Authors: Hossain MZ, Kleve MG.
Journal: Int J Nanomedicine (2011): 1475
Authors: Hossain MZ, Kleve MG.
Journal: Int J Nanomedicine (2011): 1475
Effect of glucocorticoid on production of reactive oxygen species in bone microvascular endothelial cells
Authors: Yang Y, Lou J, Li Z, Sun W, Wang B, Jia Y.
Journal: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2011): 533
Authors: Yang Y, Lou J, Li Z, Sun W, Wang B, Jia Y.
Journal: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2011): 533
Regulation of cyclooxygenase-2 and cytosolic phospholipase A2 gene expression by lipopolysaccharide through the RNA-binding protein HuR: involvement of NADPH oxidase, reactive oxygen species and mitogen-activated protein kinases
Authors: Lin WN, Lin CC, Cheng HY, Yang CM.
Journal: Br J Pharmacol (2011): 1691
Authors: Lin WN, Lin CC, Cheng HY, Yang CM.
Journal: Br J Pharmacol (2011): 1691
Effects of hypothermic storage on intracellular calcium, reactive oxygen species formation, mitochondrial function, motility, and plasma membrane integrity in striped bass (Morone saxatilis) sperm
Authors: Guthrie HD, Welch GR, Theisen DD, Woods LC, 3rd.
Journal: Theriogenology (2011): 951
Authors: Guthrie HD, Welch GR, Theisen DD, Woods LC, 3rd.
Journal: Theriogenology (2011): 951
Coenzyme Q functionalized CdTe/ZnS quantum dots for reactive oxygen species (ROS) imaging
Authors: Qin LX, Ma W, Li DW, Li Y, Chen X, Kraatz HB, James TD, Long YT.
Journal: Chemistry (2011): 5262
Authors: Qin LX, Ma W, Li DW, Li Y, Chen X, Kraatz HB, James TD, Long YT.
Journal: Chemistry (2011): 5262
The role of reactive oxygen species in WP 631-induced death of human ovarian cancer cells: a comparison with the effect of doxorubicin
Authors: Rogalska A, Gajek A, Szwed M, Jozwiak Z, Marczak A.
Journal: Toxicol In Vitro (2011): 1712
Authors: Rogalska A, Gajek A, Szwed M, Jozwiak Z, Marczak A.
Journal: Toxicol In Vitro (2011): 1712
Positive correlation between the generation of reactive oxygen species and activation/reactivation of transgene expression after hydrodynamic injections into mice
Authors: Takiguchi N, Takahashi Y, Nishikawa M, Matsui Y, Fukuhara Y, Oushiki D, Kiyose K, Hanaoka K, Nagano T, Takakura Y.
Journal: Pharm Res (2011): 702
Authors: Takiguchi N, Takahashi Y, Nishikawa M, Matsui Y, Fukuhara Y, Oushiki D, Kiyose K, Hanaoka K, Nagano T, Takakura Y.
Journal: Pharm Res (2011): 702
Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells
Authors: Zhang YH, Wu YL, Tashiro S, Onodera S, Ikejima T.
Journal: Acta Pharmacol Sin (2011): 1266
Authors: Zhang YH, Wu YL, Tashiro S, Onodera S, Ikejima T.
Journal: Acta Pharmacol Sin (2011): 1266
Application notes
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Evaluation of FLIPR Calcium Assays for Screening GPCR and Calcium Channel Targets
Fluorescent Dye AM Esters
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Evaluation of FLIPR Calcium Assays for Screening GPCR and Calcium Channel Targets
Fluorescent Dye AM Esters
FAQ
How are reactive oxygen species generated in living cells?
What's the significance of superoxide dismutase (SOD)?
Why should I use an absorbance ratio at A575nm/A605nm when using most of your Amplite® Colorimetric Assay Kits?
How should I reconstitute an NADPH standard?
How can I lyse my cells without lysing the nuclear membrane?
What's the significance of superoxide dismutase (SOD)?
Why should I use an absorbance ratio at A575nm/A605nm when using most of your Amplite® Colorimetric Assay Kits?
How should I reconstitute an NADPH standard?
How can I lyse my cells without lysing the nuclear membrane?