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ROS Brite™ 570
Optimized for Detecting Reactive Oxygen Species (ROS)
Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen. Examples include superoxide, hydroxyl radical, singlet oxygen and peroxides. ROS is highly reactive due to the presence of unpaired valence shell electrons. ROS forms as a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis. However, during times of environmental stress (e.g., UV or heat exposure), ROS levels can increase dramatically. This may result in significant damage to cell structures. Cumulatively, this is known as oxidative stress. ROS are also generated by exogenous sources such as ionizing radiation. Under conditions of oxidative stress, ROS production is dramatically increased, resulting in subsequent alteration of membrane lipids, proteins, and nucleic acids. Oxidative damage of these biomolecules is associated with aging as well as with a variety of pathological events, including atherosclerosis, carcinogenesis, ischemic reperfusion injury, and neurodegenerative disorders. ROS Brite™ 570 reagent is a new fluorogenic probe to measure oxidative stress in cells using conventional fluorescence microscopy, high-content imaging, microplate fluorometry, or flow cytometry. The cell-permeant ROS Brite™ 570 reagent is nonfluorescent and produces bright orange fluorescence upon ROS oxidation. The resulting fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry. It is an excellent alternative to CellROX™ Orange Reagent (C10443) for oxidative stress detection (CellROX™ is a trademark of ThermoFisher).
Fluorescence response of ROS Brite™ 570 to different reactive oxygen species in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 540/590 nm.
Fluorescence response of ROS Brite™ 570 to different reactive oxygen species in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 540/590 nm.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
160001 mg
Price
 
Physical properties
Molecular weight732.81
SolventDMSO
Spectral properties
Excitation (nm)555
Emission (nm)568
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationCy3/TRITC filter set
EmissionCy3/TRITC filter set
Recommended plateBlack wall/clear bottom
Instrument specification(s)Cy3/TRITC filter set
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Page updated on October 13, 2025