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ROS Brite™ 700 *Optimized for in Vivo Imaging*

Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen. Examples include superoxide, hydroxyl radical, singlet oxygen and peroxides. ROS is highly reactive due to the presence of unpaired valence shell electrons. ROS forms as a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis. However, during times of environmental stress (e.g., UV or heat exposure), ROS levels can increase dramatically. This may result in significant damage to cell structures. Cumulatively, this is known as oxidative stress. ROS are also generated by exogenous sources such as ionizing radiation. Under conditions of oxidative stress, ROS production is dramatically increased, resulting in subsequent alteration of membrane lipids, proteins, and nucleic acids. Oxidative damage of these biomolecules is associated with aging as well as with a variety of pathological events, including atherosclerosis, carcinogenesis, ischemic reperfusion injury, and neurodegenerative disorders. ROS Brite™ 700 reagent is a new fluorogenic probe to measure oxidative stress in small animals. The cell-impermeant ROS Brite™ 700 reagent is a water-soluble nonfluorescent and produces bright near-infrared fluorescence upon ROS oxidation. The resulting fluorescence can be measured using in vivo fluorescence imaging. For cell-based ROS detection, please use our cell-permemeant ROS Brite™ 570, 670 and 780 reagents.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ROS Brite™ 700 *Optimized for in Vivo Imaging* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM77.212 µL386.059 µL772.117 µL3.861 mL7.721 mL
5 mM15.442 µL77.212 µL154.423 µL772.117 µL1.544 mL
10 mM7.721 µL38.606 µL77.212 µL386.059 µL772.117 µL

Molarity calculator

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Spectrum

Citations

View all 15 citations: Citation Explorer
Nanohybrid-Based Redox Homeostasis Perturbators Escaped from Early Lysosomes toward Amplified Sensitization of Tumor Cells and Photothermally Maneuvered Pyroptosis Therapy
Authors: Yan, Xicheng and Zhang, Hao and Zhu, Hanyin and Qu, Yongyi and Wu, Yunyun and Zhu, Jing and Li, Lin and Zhang, Jixi
Journal: ACS Applied Materials \& Interfaces (2024)
Reactive Oxygen Species-Responsive Composite Fibers Regulate Oxidative Metabolism through Internal and External Factors to Promote the Recovery of Nerve Function
Authors: Zhu, Hongyi and Zhou, Liang and Tang, Jincheng and Xu, Yichang and Wang, Wei and Shi, Wenxiao and Li, Ziang and Zhang, Lichen and Ding, Zhouye and Xi, Kun and others,
Journal: Small (2024): 2401241
Nanomodulators Capable of Timely Scavenging ROS for Inflammation and Prognosis Control Following Photothermal Tumor Therapy
Authors: Wang, Shuai and Huang, Jixi and Zhu, Hanyin and Zhu, Jing and Wang, Zhenqiang and Xing, Yuxin and Xie, Xiyue and Cai, Kaiyong and Zhang, Jixi
Journal: Advanced Functional Materials (2023): 2213151
Bone microenvironment regulative hydrogels with ROS scavenging and prolonged oxygen-generating for enhancing bone repair
Authors: Sun, Han and Xu, Juan and Wang, Yangyufan and Shen, Siyu and Xu, Xingquan and Zhang, Lei and Jiang, Qing
Journal: Bioactive Materials (2023): 477--496
Melanin Nanoparticle-Actuated Redox-State Perturbation and Temporally Photoactivated Thermal Stress for Synergistic Tumor Therapy
Authors: Zhu, Hanyin and Qu, Yongyi and Wang, Shuai and Huang, Jixi and Zhu, Jing and Wang, Lu and Cai, Kaiyong and Zhang, Jixi
Journal: ACS Biomaterials Science \& Engineering (2022): 3944--3956

References

View all 91 references: Citation Explorer
Lipoxin A inhibits porphyromonas gingivalis-induced aggregation and reactive oxygen species production by modulating neutrophil-platelet interaction and CD11b expression
Authors: Borgeson E, Lonn J, Bergstrom I, Brodin VP, Ramstrom S, Nayeri F, Sarndahl E, Bengtsson T.
Journal: Infect Immun (2011): 1489
Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells
Authors: Zhang YH, Wu YL, Tashiro S, Onodera S, Ikejima T.
Journal: Acta Pharmacol Sin (2011): 1266
Positive correlation between the generation of reactive oxygen species and activation/reactivation of transgene expression after hydrodynamic injections into mice
Authors: Takiguchi N, Takahashi Y, Nishikawa M, Matsui Y, Fukuhara Y, Oushiki D, Kiyose K, Hanaoka K, Nagano T, Takakura Y.
Journal: Pharm Res (2011): 702
The role of reactive oxygen species in WP 631-induced death of human ovarian cancer cells: a comparison with the effect of doxorubicin
Authors: Rogalska A, Gajek A, Szwed M, Jozwiak Z, Marczak A.
Journal: Toxicol In Vitro (2011): 1712
Coenzyme Q functionalized CdTe/ZnS quantum dots for reactive oxygen species (ROS) imaging
Authors: Qin LX, Ma W, Li DW, Li Y, Chen X, Kraatz HB, James TD, Long YT.
Journal: Chemistry (2011): 5262
Page updated on November 5, 2024

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Catalog Number16004
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Physical properties

Molecular weight

1295.14

Solvent

DMSO

Spectral properties

Excitation (nm)

682

Emission (nm)

701

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Fluorescence response of ROS Brite&trade; 700&nbsp;to different reactive oxygen species in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 670/700 nm.
Fluorescence response of ROS Brite&trade; 700&nbsp;to different reactive oxygen species in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 670/700 nm.
Fluorescence response of ROS Brite&trade; 700&nbsp;to different reactive oxygen species in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 670/700 nm.