ROS Brite™ 700 *Optimized for in Vivo Imaging*

ROS Brite™ 700

Optimized for in Vivo Imaging

Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen. Examples include superoxide, hydroxyl radical, singlet oxygen and peroxides. ROS is highly reactive due to the presence of unpaired valence shell electrons. ROS forms as a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis. However, during times of environmental stress (e.g., UV or heat exposure), ROS levels can increase dramatically. This may result in significant damage to cell structures. Cumulatively, this is known as oxidative stress. ROS are also generated by exogenous sources such as ionizing radiation. Under conditions of oxidative stress, ROS production is dramatically increased, resulting in subsequent alteration of membrane lipids, proteins, and nucleic acids. Oxidative damage of these biomolecules is associated with aging as well as with a variety of pathological events, including atherosclerosis, carcinogenesis, ischemic reperfusion injury, and neurodegenerative disorders. ROS Brite™ 700 reagent is a new fluorogenic probe to measure oxidative stress in small animals. The cell-impermeant ROS Brite™ 700 reagent is a water-soluble nonfluorescent and produces bright near-infrared fluorescence upon ROS oxidation. The resulting fluorescence can be measured using in vivo fluorescence imaging. For cell-based ROS detection, please use our cell-permemeant ROS Brite™ 570, 670 and 780 reagents.

Fluorescence response of ROS Brite™ 700 to different reactive oxygen species in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 670/700 nm.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
16004	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ROS Brite™ 700 *Optimized for in Vivo Imaging* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	77.212 µL	386.059 µL	772.117 µL	3.861 mL	7.721 mL
5 mM	15.442 µL	77.212 µL	154.423 µL	772.117 µL	1.544 mL
10 mM	7.721 µL	38.606 µL	77.212 µL	386.059 µL	772.117 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1295.14 g/mol	=	mL	x	mM	=	mmol

Citations

View all 19 citations: Citation Explorer

An Immunomodulating Regenerating Hydrogel That Rescues the Oxidative Microenvironment and Reverses Cell Senescence for Osteoporotic Bone Defects

Authors:

Wang, Wei and Zhang, Xing and Zhang, Lichen and Li, Ziang and Wang, Lingjun and Jiang, Xinzhao and Wu, Jie and Zhou, Liang and Guo, Qiangqiang and Huang, Yiyang and others,

Journal:

ACS nano (2025)

Camouflaged Nanorobots Target and Drive the Subcellular Organelle Crosstalk Pattern of Innate Immune Cells to Promote Neural Regeneration

Authors:

Xi, Kun and Guo, Qiangqiang and Wang, Wei and Jiang, Xinzhao and Tang, Jincheng and Cai, Feng and Wang, Wenbo and Zhang, Lichen and Li, Ziang and Huang, Yiyang and others,

Signal Converter-Based Therapy Platform Promoting Aging Bone Healing by Improving Permeability of the Mitochondrial Membrane

Authors:

Huang, Yiyang and Mao, Jiannan and Li, Ziang and Wang, Wenbo and Ni, Zhengxia and Cai, Feng and Tang, Jincheng and Wang, Wei and Zhang, Lichen and Zhou, Liang and others,

Journal:

Advanced Materials (2025): 2500156

Dual inhibition of oxidative phosphorylation and glycolysis exerts a synergistic antitumor effect on colorectal and gastric cancer by creating energy depletion and preventing metabolic switch

Authors:

Aisu, Yuki and Oshima, Nobu and Hyodo, Fuminori and Elhelaly, Abdelazim Elsayed and Masuo, Akihiko and Okada, Tomoaki and Hisamori, Shigeo and Tsunoda, Shigeru and Hida, Koya and Morimoto, Tomonori and others,

Journal:

PloS one (2024): e0309700

Nanohybrid-Based Redox Homeostasis Perturbators Escaped from Early Lysosomes toward Amplified Sensitization of Tumor Cells and Photothermally Maneuvered Pyroptosis Therapy

Authors:

Yan, Xicheng and Zhang, Hao and Zhu, Hanyin and Qu, Yongyi and Wu, Yunyun and Zhu, Jing and Li, Lin and Zhang, Jixi

Journal:

ACS Applied Materials \& Interfaces (2024)

References

View all 91 references: Citation Explorer

Positive correlation between the generation of reactive oxygen species and activation/reactivation of transgene expression after hydrodynamic injections into mice

Authors:

Takiguchi N, Takahashi Y, Nishikawa M, Matsui Y, Fukuhara Y, Oushiki D, Kiyose K, Hanaoka K, Nagano T, Takakura Y.

Journal:

Pharm Res (2011): 702

Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells

Authors:

Zhang YH, Wu YL, Tashiro S, Onodera S, Ikejima T.

Journal:

Acta Pharmacol Sin (2011): 1266

The role of reactive oxygen species in WP 631-induced death of human ovarian cancer cells: a comparison with the effect of doxorubicin

Authors:

Rogalska A, Gajek A, Szwed M, Jozwiak Z, Marczak A.

Journal:

Toxicol In Vitro (2011): 1712

Coenzyme Q functionalized CdTe/ZnS quantum dots for reactive oxygen species (ROS) imaging

Authors:

Qin LX, Ma W, Li DW, Li Y, Chen X, Kraatz HB, James TD, Long YT.

Journal:

Chemistry (2011): 5262

Effects of hypothermic storage on intracellular calcium, reactive oxygen species formation, mitochondrial function, motility, and plasma membrane integrity in striped bass (Morone saxatilis) sperm

Authors:

Guthrie HD, Welch GR, Theisen DD, Woods LC, 3rd.

Journal:

Theriogenology (2011): 951

Physical properties

Molecular weight	1295.14
Solvent	DMSO

Spectral properties

Excitation (nm)	682
Emission (nm)	701

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 8, 2025