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ROS Brite™ DHCF

Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen. Examples include superoxide, hydroxyl radical, singlet oxygen and peroxides. ROS is highly reactive due to the presence of unpaired valence shell electrons. ROS forms as a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis. However, during times of environmental stress (e.g., UV or heat exposure), ROS levels can increase dramatically. This may result in significant damage to cell structures. Cumulatively, this is known as oxidative stress. ROS Brite™ DHCF has similar redox properties to those of 2',7'-Dichlorodihydrofluorescein diacetate with significantly red-shifted spectra. ROS Brite™ DHCF is hydrolyzed by cellular esterases to generate the non-fluorescent reduced form that is then oxidized to generate the highly fluorescent free dye primarily by H2O2. ROS Brite™ DHCF might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. This probe can be conveniently used to monitoring cellular redox processes for multiplexing assays with FITC-labeled antibodies or GFP cell lines. The oxidized product is highly fluorescent in cells. ROS Brite™ DHCF provides a valuable tool for investigating oxidative stress in various pathologies.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ROS Brite™ DHCF to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM142.552 µL712.758 µL1.426 mL7.128 mL14.255 mL
5 mM28.51 µL142.552 µL285.103 µL1.426 mL2.851 mL
10 mM14.255 µL71.276 µL142.552 µL712.758 µL1.426 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Citations

View all 2 citations: Citation Explorer
Toxin-Enabled “On-Demand” Liposomes for Enhanced Phototherapy to Treat and Protect against Methicillin-Resistant Staphylococcus aureus Infection
Authors: Zhuge, Deli and Chen, Mengchun and Yang, Xuewei and Zhang, Xufei and Yao, Lulu and Li, Li and Wang, Haonan and Chen, Hao and Yin, Qingqing and Tian, Dongyan and others,
Journal: Small (2022): 2203292
Oxidative Stress--An Update and Insight in the Romanian Family Physician’s Adoption of the Concept
Authors: Berghea, Florian and Berghea, Camelia Elena and Abobului, Mihai
Journal: Internal Medicine : 11--15

References

View all 46 references: Citation Explorer
Nonthermal plasma induces head and neck cancer cell death: the potential involvement of mitogen-activated protein kinase-dependent mitochondrial reactive oxygen species
Authors: Kang SU, Cho JH, Chang JW, Shin YS, Kim KI, Park JK, Yang SS, Lee JS, Moon E, Lee K, Kim CH.
Journal: Cell Death Dis (2014): e1056
An oxidative stress mechanism of shikonin in human glioma cells
Authors: Yang JT, Li ZL, Wu JY, Lu FJ, Chen CH.
Journal: PLoS One (2014): e94180
Low Amounts of Mitochondrial Reactive Oxygen Species Define Human Sperm Quality
Authors: Marques M, Sousa AP, Paiva A, Almeida-Santos T, Ramalho-Santos J.
Journal: Reproduction. (2014)
Subneurotoxic copper(II)-induced NF-kappaB-dependent microglial activation is associated with mitochondrial ROS
Authors: Hu Z, Yu F, Gong P, Qiu Y, Zhou W, Cui Y, Li J, Chen H.
Journal: Toxicol Appl Pharmacol (2014): 95
HPLC-based monitoring of products formed from hydroethidine-based fluorogenic probes--the ultimate approach for intra- and extracellular superoxide detection
Authors: Kalyanaraman B, Dranka BP, Hardy M, Michalski R, Zielonka J.
Journal: Biochim Biophys Acta (2014): 739
Page updated on October 11, 2024

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Catalog Number16053
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Physical properties

Molecular weight

701.50

Solvent

DMSO

Spectral properties

Excitation (nm)

560

Emission (nm)

575

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Fluorescence images of ROS measurement in HeLa cells using ROS Brite&trade; DHCF (Cat# 16053). H<sub>2</sub>O<sub>2</sub> Treatment: Cells were incubated with ROS Brite&trade; DHCF for 1 hour, then treated with 1 mM H<sub>2</sub>O<sub>2</sub> at 37 &deg;C for 30 minutes. Untreated Control: HeLa cells were incubated with ROS Brite&trade; DHCF at 37 &deg;C for 1 hour without H<sub>2</sub>O<sub>2</sub> treatment. The fluorescence signal was measured using fluorescence microscope with a TRITC filter.
Fluorescence images of ROS measurement in HeLa cells using ROS Brite&trade; DHCF (Cat# 16053). H<sub>2</sub>O<sub>2</sub> Treatment: Cells were incubated with ROS Brite&trade; DHCF for 1 hour, then treated with 1 mM H<sub>2</sub>O<sub>2</sub> at 37 &deg;C for 30 minutes. Untreated Control: HeLa cells were incubated with ROS Brite&trade; DHCF at 37 &deg;C for 1 hour without H<sub>2</sub>O<sub>2</sub> treatment. The fluorescence signal was measured using fluorescence microscope with a TRITC filter.
Fluorescence images of ROS measurement in HeLa cells using ROS Brite&trade; DHCF (Cat# 16053). H<sub>2</sub>O<sub>2</sub> Treatment: Cells were incubated with ROS Brite&trade; DHCF for 1 hour, then treated with 1 mM H<sub>2</sub>O<sub>2</sub> at 37 &deg;C for 30 minutes. Untreated Control: HeLa cells were incubated with ROS Brite&trade; DHCF at 37 &deg;C for 1 hour without H<sub>2</sub>O<sub>2</sub> treatment. The fluorescence signal was measured using fluorescence microscope with a TRITC filter.