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ROS Brite™ DHCF
Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen. Examples include superoxide, hydroxyl radical, singlet oxygen and peroxides. ROS is highly reactive due to the presence of unpaired valence shell electrons. ROS forms as a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis. However, during times of environmental stress (e.g., UV or heat exposure), ROS levels can increase dramatically. This may result in significant damage to cell structures. Cumulatively, this is known as oxidative stress. ROS Brite™ DHCF has similar redox properties to those of 2',7'-Dichlorodihydrofluorescein diacetate with significantly red-shifted spectra. ROS Brite™ DHCF is hydrolyzed by cellular esterases to generate the non-fluorescent reduced form that is then oxidized to generate the highly fluorescent free dye primarily by H2O2. ROS Brite™ DHCF might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. This probe can be conveniently used to monitoring cellular redox processes for multiplexing assays with FITC-labeled antibodies or GFP cell lines. The oxidized product is highly fluorescent in cells. ROS Brite™ DHCF provides a valuable tool for investigating oxidative stress in various pathologies.
Fluorescence images of ROS measurement in HeLa cells using ROS Brite&trade; DHCF (Cat# 16053). H<sub>2</sub>O<sub>2</sub> Treatment: Cells were incubated with ROS Brite&trade; DHCF for 1 hour, then treated with 1 mM H<sub>2</sub>O<sub>2</sub> at 37 &deg;C for 30 minutes. Untreated Control: HeLa cells were incubated with ROS Brite&trade; DHCF at 37 &deg;C for 1 hour without H<sub>2</sub>O<sub>2</sub> treatment. The fluorescence signal was measured using fluorescence microscope with a TRITC filter.
Fluorescence images of ROS measurement in HeLa cells using ROS Brite&trade; DHCF (Cat# 16053). H<sub>2</sub>O<sub>2</sub> Treatment: Cells were incubated with ROS Brite&trade; DHCF for 1 hour, then treated with 1 mM H<sub>2</sub>O<sub>2</sub> at 37 &deg;C for 30 minutes. Untreated Control: HeLa cells were incubated with ROS Brite&trade; DHCF at 37 &deg;C for 1 hour without H<sub>2</sub>O<sub>2</sub> treatment. The fluorescence signal was measured using fluorescence microscope with a TRITC filter.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
160531 mg
Price
 
Physical properties
Molecular weight701.50
SolventDMSO
Spectral properties
Excitation (nm)560
Emission (nm)575
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 23, 2025