ROX Reference Dye 50X fluorescence reference solution for PCR reactions

Name: ROX Reference Dye *50X fluorescence reference solution for PCR reactions*
Brand: AAT Bioquest
SKU: 400
Price: 227 USD
Availability: InStock

Documents

Certificate of origin

Safety data sheet

Product protocol

Certificate of analysis

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Polymerase Chain Reaction (PCR)

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ROX Reference Dye for Real-Time PCR

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Application notes

A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells

Abbreviation of Common Chemical Compounds Related to Peptides

Annexin V

Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis

Calcein

AssayWise

Glycerol 3-Phosphate Assays

Nucleic Acid Quantification

Hydroxyl Radical Detection

Thiol Detection

Nucleic Acid Detection, Quantification and Imaging

ROX Reference Dye is equivalent to 12223-012 (Life Technology). It provides users of the ABI PRISM® 7700 a convenient method for normalizing fluorescent reporter signal in real-time quantitative PCR or RT-PCR without modifying the instrument's default analysis parameters. ROX Reference Dye is supplied at 50X concentration. ROX Reference Dye is made of highly purified 5-ROX glycine. Compared to the standard ROX dyes 5-ROX glycine has improved stability.

Ordering information

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Catalog Number	400
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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

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Physical properties

Molecular weight	N/A
Solvent	Water

Spectral properties

Correction Factor (280 nm)	0.168
Extinction coefficient (cm ^-1 M ^-1)	82000
Excitation (nm)	578
Emission (nm)	604
Quantum yield	0.94¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Spectrum

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References

View all 11 references: Citation Explorer

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926

Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412

Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488

Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834

Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312

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