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RPE-iFluor® 750-streptavidin conjugate
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This RPE-iFluor® 750-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with RPE-iFluor® 750 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a Cy7® filter set. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.
Performance of  RPE-iFluor® 750-streptavidin conjugate (Cat#16907) and streptavidin-PE/Cy7 (Vendor B). HL-60 cells were stained with or without CD45 antibody (1ug/ml) for 30min, and followed by streptavidin conjugate (5ug/ml) for 30min. (Red peak: RPE-iFluor® 750-streptavidin (cat#16907), Green peak: SA-PE/Cy7 (Vendor B)).
Performance of  RPE-iFluor® 750-streptavidin conjugate (Cat#16907) and streptavidin-PE/Cy7 (Vendor B). HL-60 cells were stained with or without CD45 antibody (1ug/ml) for 30min, and followed by streptavidin conjugate (5ug/ml) for 30min. (Red peak: RPE-iFluor® 750-streptavidin (cat#16907), Green peak: SA-PE/Cy7 (Vendor B)).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
16907100 ug
Price
 
Physical properties
Molecular weight~292000
SolventWater
Spectral properties
Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)565
Emission (nm)778
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12352200
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Page updated on October 8, 2025