Name: Screen Quest™ 10X cell staining buffer with Phenol Red Plus™
Brand: AAT Bioquest
SKU: 36300
Price: 118 USD
Availability: InStock

Screen Quest™ 10X cell staining buffer with Phenol Red Plus™

Screen Quest™ 10X Cell Staining Buffer with Phenol Red Plus™ is a 10X concentrated buffer optimized for fluorescence cell imaging workflows.

Fluorescence imaging support: Optimized for fluorescence cell imaging workflows
Phenol Red Plus™ formulation: Includes Phenol Red Plus™ for cell staining and imaging applications
Signal enhancement: May significantly enhance imaging signal in selected staining workflows
Research applications: Suitable for fluorescence cell staining and microscopy-based cell imaging workflows

ATP dose response was measured in CHO-M1 cells with Cal-520 ™AM. CHO-M1 cells were seeded overnight at 50,000 cells/100 µL/well in a 96-well black wall/clear bottom costar plate. 100 µL of 10ug/ml Cal-520 ™AM in HH Buffer with  1X Phenol Red Plus™ cell staining buffer was added and incubated for 60 min at 37oC. ATP (50µL/well) was added to achieve  the final indicated concentrations.

Protocol

SDS

Catalog	Size	Price	Quantity
36300	10 mL	Price

Overview

Screen Quest™ 10X Cell Staining Buffer with Phenol Red Plus™ is a concentrated buffer optimized for fluorescence cell imaging. The buffer is designed for use in cell staining workflows where fluorescence imaging signal quality is important.

In selected cases, Screen Quest™ 10X Cell Staining Buffer with Phenol Red Plus™ can significantly enhance the imaging signal. The buffer is supplied as a 10X concentrate and should be diluted to 1X with PBS before use. Screen Quest™ 10X Cell Staining Buffer with Phenol Red Plus™ is suitable for fluorescence cell staining and microscopy-based cell imaging applications.

References

View all 26 references: Citation Explorer

Novel fluo-4 analogs for fluorescent calcium measurements

Authors:

Martin VV, Beierlein M, Morgan JL, Rothe A, Gee KR.

Journal:

Cell Calcium (2004): 509

Kinetic characterization of novel NR2B antagonists using fluorescence detection of calcium flux

Authors:

Bednar B, Cunningham ME, Kiss L, Cheng G, McCauley JA, Liverton NJ, Koblan KS.

Journal:

J Neurosci Methods (2004): 247

Flow cytometric kinetic assay of calcium mobilization in whole blood platelets using Fluo-3 and CD41

Authors:

do Ceu Monteiro M, Sansonetty F, Goncalves MJ, O'Connor JE.

Journal:

Cytometry (1999): 302

Amplitude distribution of calcium sparks in confocal images: theory and studies with an automatic detection method

Authors:

Cheng H, Song LS, Shirokova N, Gonzalez A, Lakatta EG, Rios E, Stern MD.

Journal:

Biophys J (1999): 606

A simple numerical model of calcium spark formation and detection in cardiac myocytes

Authors:

Smith GD, Keizer JE, Stern MD, Lederer WJ, Cheng H.

Journal:

Biophys J (1998): 15

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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