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Screen Quest™ 10X cell staining buffer with Phenol Red Plus™

ATP dose response was measured in CHO-M1 cells with Cal-520 ™AM. CHO-M1 cells were seeded overnight at 50,000 cells/100 µL/well in a 96-well black wall/clear bottom costar plate. 100 µL of 10ug/ml Cal-520 ™AM in HH Buffer with  1X Phenol Red Plus™ cell staining buffer was added and incubated for 60 min at 37oC. ATP (50µL/well) was added to achieve  the final indicated concentrations.
ATP dose response was measured in CHO-M1 cells with Cal-520 ™AM. CHO-M1 cells were seeded overnight at 50,000 cells/100 µL/well in a 96-well black wall/clear bottom costar plate. 100 µL of 10ug/ml Cal-520 ™AM in HH Buffer with  1X Phenol Red Plus™ cell staining buffer was added and incubated for 60 min at 37oC. ATP (50µL/well) was added to achieve  the final indicated concentrations.
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Catalog Number36300
Unit Size
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Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

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Ready-to-use buffer that is optimized for fluorescence cell imaing. In some cases, this buffer significantly enhances imaging signal.

References


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