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Screen Quest™ 10X cell staining buffer with Phenol Red Plus™
Screen Quest™ 10X cell staining buffer with Phenol Red Plus™ is a ready-to-use buffer optimized for fluorescence cell imaging. In some cases, this buffer significantly enhances the imaging signal. Screen Quest™ 10X cell staining buffer with Phenol Red Plus™ is 10X concentrated and should be diluted to 1X with PBS before use.
ATP dose response was measured in CHO-M1 cells with Cal-520 ™AM. CHO-M1 cells were seeded overnight at 50,000 cells/100 µL/well in a 96-well black wall/clear bottom costar plate. 100 µL of 10ug/ml Cal-520 ™AM in HH Buffer with  1X Phenol Red Plus™ cell staining buffer was added and incubated for 60 min at 37oC. ATP (50µL/well) was added to achieve  the final indicated concentrations.
ATP dose response was measured in CHO-M1 cells with Cal-520 ™AM. CHO-M1 cells were seeded overnight at 50,000 cells/100 µL/well in a 96-well black wall/clear bottom costar plate. 100 µL of 10ug/ml Cal-520 ™AM in HH Buffer with  1X Phenol Red Plus™ cell staining buffer was added and incubated for 60 min at 37oC. ATP (50µL/well) was added to achieve  the final indicated concentrations.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
3630010 mL
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Page updated on October 13, 2025