logo
AAT Bioquest

Screen Quest™ CHO-Ga16- Chemokine (C-C) Receptor 2B Cells

Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageLiquid nitrogen
UNSPSC12352200
Related products
Screen Quest™ Membrane Potential Assay Kit *Orange Fluorescence*
Screen Quest™ Membrane Potential Assay Kit *Red Fluorescence*
Screen Quest™ 10X cell staining buffer with Phenol Red Plus™
Screen Quest™ 10X calcium assay buffer with Phenol Red Plus™
Screen Quest™ Luminometric Calcium Assay Kit
Screen Quest™ Fluo-8 Medium Removal Calcium Assay Kit *Optimized for Difficult Cell Lines*
Screen Quest™ Fluo-8 No Wash Calcium Assay Kit
Screen Quest™ Fura-2 No Wash Calcium Assay Kit
Screen Quest™ Rhod-4 No Wash Calcium Assay Kit *Medium Removal*
Screen Quest™ Rhod-4 No Wash Calcium Assay Kit
Screen Quest™ Fluorimetric MDR Assay Kit
Screen Quest™ Colorimetric Chloride Channel Assay Kit
Screen Quest™ Colorimetric ELISA cAMP Assay Kit
Screen Quest™ Fluorimetric ELISA cAMP Assay Kit
Screen Quest™ TR-FRET No Wash cAMP Assay Kit
Screen Quest™ Live Cell cAMP Assay Service Pack
Screen Quest™ Fluorimetric Fatty Acid Uptake Assay Kit
Screen Quest™ Fluorimetric Glucose Uptake Assay Kit
Screen Quest™ Colorimetric Glucose Uptake Assay Kit
Screen Quest™ HEK-CNGC-Amylin 3 Receptor Cells
Screen Quest™ HEK-CNGC-Cannabinoid Receptor 1 Cells
Screen Quest™ HEK-CNGC-Chemokine (C-X-C motif) Receptor 4 Cells
Screen Quest™ HEK-CNGC-Dopamine Receptor 1 (DRD1) Cells
Screen Quest™ HEK-CNGC-Glucagon-like Receptor 1 (GLP1R) Cells
Screen Quest™ HEK-CNGC-Opiate Receptor-like 1 (ORL1) Cells
Screen Quest™ Human Nociceptin Receptor Ga16 coupled CHO Cells (NOP-Ga16)
Screen Quest™ CHO-Gqi Chimera Cell line
Screen Quest™ CHO-Gqo Chimera Cell line
Screen Quest™ CHO-Gqz Chimera Cell line
Screen Quest™ CHO-Gqs Chimera Cell line
Screen Quest™ CHO-Ga16 Chimera Cell line
Screen Quest™ Fluo-4 No Wash Calcium Assay Kit
Screen Quest™ No Wash Potassium Channel Assay Kit
Screen Quest™ Calbryte-520 Probenecid-Free and Wash-Free Calcium Assay Kit
Screen Quest™ Calbryte-590 Probenecid-Free and Wash-Free Calcium Assay Kit
Show More (25)

OverviewpdfSDSpdfProtocol


Over 60% of the known G-protein-coupled receptors (GPCRs) signal through pathways other than Gq which lead to an increase in intracellular calcium. As genomics reveals more G-protein-coupled receptor targets this trend continues to increase. Screen Quest™ cell lines enable to investigate GPCR that do not conventionally couple through intracellular calcium. Screen Quest™ cell lines are based on a series of G-protein chimeras, including the promiscuous G-protein, Gα16. The chimeras consist of the alpha subunit of a Gq-protein complex whose 5 carboxy-terminal amino acids have been replaced with those from one of the other G-proteins (either Gαs, Gαi, Gαo, or Gαz). These amino acids are responsible for the coupling of the receptor to its G-protein. Co-expression of these chimeras with specific non-Gq-coupled receptors may result in the generation of an intracellular calcium signal upon receptor stimulation. Screen Quest™ CHO-Ga16- Chemokine (C-C) Receptor 2B (CHO-Ga16-CCR2B) cell line is CHO-K1 cells stably transfected with both the promiscuous G-protein, Gα16 and Chemokine (C-C) Receptor 2B receptor (CCR2B). The constitutively expressed Gα16 protein in the cells allows this receptor which normally act through the cAMP pathway, to couple to Gq signal transduction and mobilized intracellular calcium. Activation of the CCR2B in these cells by specific ligands can be detected using calcium sensitive dyes such as Calbryte 520 AM, Cal-520 AM, Fluo-8 AM, or Fluo-4 AM and the corresponding no wash calcium kits. This cell line has been successfully used in drug discovery and screening environments for studying GPCRs that do not conventionally couple through intracellular calcium. It has been effectively used with FLIPR and FDSS systems.

Citations


View all 2 citations: Citation Explorer
Activation of P2X7 and P2Y11 purinergic receptors inhibits migration and normalizes tumor-derived endothelial cells via cAMP signaling
Authors: Avanzato, D and Genova, T and Pla, A Fiorio and Bernardini, M and Bianco, S and Bussolati, B and Mancardi, D and Giraudo, E and Maione, F and Cassoni, P and others, undefined
Journal: Scientific Reports (2016)
The M2 muscarinic receptors are essential for signaling in the heart left ventricle during restraint stress in mice
Authors: Tomankova, Hana and Valuskova, Paulina and Varejkova, Eva and Rotkova, Jana and Benes, Jan and Myslivecek, Jaromir
Journal: Stress (2015)

References


View all 132 references: Citation Explorer
cAMP-Induced Histones H3 Dephosphorylation Is Independent of PKA and MAP Kinase Activations and Correlates With mTOR Inactivation
Authors: Rodriguez P, Rojas J.
Journal: J Cell Biochem (2016): 741
Changes in the Arabidopsis thaliana Proteome Implicate cAMP in Biotic and Abiotic Stress Responses and Changes in Energy Metabolism
Authors: Alqurashi M, Gehring C, Marondedze C.
Journal: Int J Mol Sci (2016): 852
Role of the cAMP Pathway in Glucose and Lipid Metabolism
Authors: Ravnskjaer K, Madiraju A, Montminy M.
Journal: Handb Exp Pharmacol (2016): 29
Odor-induced cAMP production in Drosophila melanogaster olfactory sensory neurons
Authors: Miazzi F, Hansson BS, Wicher D.
Journal: J Exp Biol (2016): 1798
A cardiac mitochondrial cAMP signaling pathway regulates calcium accumulation, permeability transition and cell death
Authors: Wang Z, Liu D, Varin A, Nicolas V, Courilleau D, Mateo P, Caubere C, Rouet P, Gomez AM, V and ecasteele G, Fischmeister R, Brenner C.
Journal: Cell Death Dis (2016): e2198
The pleiotropic role of exchange protein directly activated by cAMP 1 (EPAC1) in cancer: implications for therapeutic intervention
Authors: Almahariq M, Mei FC, Cheng X.
Journal: Acta Biochim Biophys Sin (Shanghai) (2016): 75
A cAMP Biosensor-Based High-Throughput Screening Assay for Identification of Gs-Coupled GPCR Ligands and Phosphodiesterase Inhibitors
Authors: Vedel L, Brauner-Osborne H, Mathiesen JM.
Journal: J Biomol Screen (2015): 849
Electrostatic steering enhances the rate of cAMP binding to phosphodiesterase: Brownian dynamics modeling
Authors: Huang YM, Huber G, McCammon JA.
Journal: Protein Sci (2015): 1884
Cardiac cAMP: production, hydrolysis, modulation and detection
Authors: Boularan C, Gales C.
Journal: Front Pharmacol (2015): 203
In vivo model with targeted cAMP biosensor reveals changes in receptor-microdomain communication in cardiac disease
Authors: Sprenger JU, Perera RK, Steinbrecher JH, Lehnart SE, Maier LS, Hasenfuss G, Nikolaev VO.
Journal: Nat Commun (2015): 6965