Screen Quest™ Colorimetric Chloride Channel Assay Kit

Chloride channels have a variety of important physiological and cellular functions that include regulation of pH, volume homeostasis, organic solute transport, cell migration, cell proliferation and differentiation. Chloride channels represent valuable drug targets. A number of chronic disease states such as cystic fibrosis and Bartter's syndrome are due to defects in chloride channel functions. However, the existing technologies for screening chloride channel modulators are a compromise between throughput, sensitivity and physiological relevance. Screen Quest™ Colorimetric Chloride Channel Assay Kit provides a sensitive and robust colorimetric method for studying chloride channels. The assay is based on our proprietary iodide indicator (Iodide Blue™) for measuring iodide concentration, as low as 30 nM of iodide was detected by this assay. Screen Quest™ Chloride Channel Assay Kit provides an optimized assay method for monitoring chloride channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation.

Protocol

COA

Catalog	Size	Price	Quantity
36350	10 Plates	Price
36351	100 Plates	Price

References

View all 44 references: Citation Explorer

Identification of metastasis-associated proteins involved in gallbladder carcinoma metastasis by proteomic analysis and functional exploration of chloride intracellular channel 1

Authors:

Wang JW, Peng SY, Li JT, Wang Y, Zhang ZP, Cheng Y, Cheng DQ, Weng WH, Wu XS, Fei XZ, Quan ZW, Li JY, Li SG, Liu YB.

Journal:

Cancer Lett (2009): 71

Oxidation promotes insertion of the CLIC1 chloride intracellular channel into the membrane

Authors:

Goodchild SC, Howell MW, Cordina NM, Littler DR, Breit SN, Curmi PM, Brown LJ.

Journal:

Eur Biophys J (2009): 129

Mutation-specific potency and efficacy of cystic fibrosis transmembrane conductance regulator chloride channel potentiators

Authors:

Caputo A, Hinzpeter A, Caci E, Pedemonte N, Arous N, Di Duca M, Zegarra-Moran O, Fanen P, Galietta LJ.

Journal:

J Pharmacol Exp Ther (2009): 783

Activation of a chloride channel by a trophic ligand is required for development of the mouse preimplantation embryo in vitro

Authors:

Li Y, O'Neill C, Day ML.

Journal:

Biol Reprod (2009): 759

ANO2 is the cilial calcium-activated chloride channel that may mediate olfactory amplification

Authors:

Stephan AB, Shum EY, Hirsh S, Cygnar KD, Reisert J, Zhao H.

Journal:

Proc Natl Acad Sci U S A (2009): 11776

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Absorbance microplate reader
Absorbance	630, 380, or 405 nm
Recommended plate	Clear bottom

Documents

Protocol
Safety Data Sheet (Catalog 36350)
Safety Data Sheet (Catalog 36351)
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
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Custom size	Inquire
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 22, 2025

Component A: Iodide Blue™ Sensor	1 bottle (50 mL)
Component B: Iodide Sensor Enhancer (100X)	1 vial (0.5 mL)
Component C: I- Loading Buffer	1 bottle (100 mL)
Component D: Cell Lysis Buffer (10X)	1 bottle (5 mL)

Component A: Iodide Blue™ Sensor	1 bottle (50 mL)
Component B: Iodide Sensor Enhancer (100X)	1 vial (0.5 mL)
Component C: I- Loading Buffer	1 bottle (100 mL)
Component D: Cell Lysis Buffer (10X)	1 bottle (5 mL)