Screen Quest™ Colorimetric Chloride Channel Assay Kit

Chloride channels have a variety of important physiological and cellular functions that include regulation of pH, volume homeostasis, organic solute transport, cell migration, cell proliferation and differentiation. Chloride channels represent valuable drug targets. A number of chronic disease states such as cystic fibrosis and Bartter's syndrome are due to defects in chloride channel functions. However, the existing technologies for screening chloride channel modulators are a compromise between throughput, sensitivity and physiological relevance. Screen Quest™ Colorimetric Chloride Channel Assay Kit provides a sensitive and robust colorimetric method for studying chloride channels. The assay is based on our proprietary iodide indicator (Iodide Blue™) for measuring iodide concentration, as low as 30 nM of iodide was detected by this assay. Screen Quest™ Chloride Channel Assay Kit provides an optimized assay method for monitoring chloride channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation.

Protocol

Catalog	Size	Price	Quantity
36350	10 Plates	Price
36351	100 Plates	Price

References

View all 44 references: Citation Explorer

Identification of metastasis-associated proteins involved in gallbladder carcinoma metastasis by proteomic analysis and functional exploration of chloride intracellular channel 1

Authors:

Wang JW, Peng SY, Li JT, Wang Y, Zhang ZP, Cheng Y, Cheng DQ, Weng WH, Wu XS, Fei XZ, Quan ZW, Li JY, Li SG, Liu YB.

Journal:

Cancer Lett (2009): 71

Oxidation promotes insertion of the CLIC1 chloride intracellular channel into the membrane

Authors:

Goodchild SC, Howell MW, Cordina NM, Littler DR, Breit SN, Curmi PM, Brown LJ.

Journal:

Eur Biophys J (2009): 129

Mutation-specific potency and efficacy of cystic fibrosis transmembrane conductance regulator chloride channel potentiators

Authors:

Caputo A, Hinzpeter A, Caci E, Pedemonte N, Arous N, Di Duca M, Zegarra-Moran O, Fanen P, Galietta LJ.

Journal:

J Pharmacol Exp Ther (2009): 783

Activation of a chloride channel by a trophic ligand is required for development of the mouse preimplantation embryo in vitro

Authors:

Li Y, O'Neill C, Day ML.

Journal:

Biol Reprod (2009): 759

ANO2 is the cilial calcium-activated chloride channel that may mediate olfactory amplification

Authors:

Stephan AB, Shum EY, Hirsh S, Cygnar KD, Reisert J, Zhao H.

Journal:

Proc Natl Acad Sci U S A (2009): 11776

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	630, 380, or 405 nm
Recommended plate	Clear bottom

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Page updated on August 3, 2026

Component A: Iodide Blue™ Sensor	1 bottle (50 mL)
Component B: Iodide Sensor Enhancer (100X)	1 vial (0.5 mL)
Component C: I- Loading Buffer	1 bottle (100 mL)
Component D: Cell Lysis Buffer (10X)	1 bottle (5 mL)

Component A: Iodide Blue™ Sensor	1 bottle (50 mL)
Component B: Iodide Sensor Enhancer	1 vial (0.5 mL)
Component C: I- Loading Buffer	1 bottle (100 mL)
Component D: Cell Lysis Buffer	1 bottle (5 mL)