Name: Screen Quest™ Colorimetric Glucose Uptake Assay Kit
Brand: AAT Bioquest
SKU: 36503
Availability: InStock

Screen Quest™ Colorimetric Glucose Uptake Assay Kit

Screen Quest™ Colorimetric Glucose Uptake Assay Kit is a non-radioactive colorimetric assay kit for measuring 2-deoxyglucose uptake in tissues or cultured cells.

2-DG uptake readout: Measures cellular uptake of 2-deoxyglucose as an indicator of glucose transport activity
Non-radioactive format: Provides an alternative to tritium- or C14-labeled 2-DG uptake assays
Enzyme-coupled detection: Detects accumulated 2-DG-6-phosphate through NADPH generation and a chromogenic NADPH sensor
Research applications: Suitable for glucose uptake analysis, glucose transporter studies, metabolic regulation research, and insulin resistance studies

Measurement of 2DG uptake in differentiated 3T3-L1 adipocytes and 3T3-L1 fibroblasts. Assays were performed with Screen Quest™ Colorimetric Glucose Uptake Assay Kit in a black wall/clear bottom cell culture Poly-D lysine plate using a SpectraMax (Molecular Devices) microplate reader. (A: Negative Control, no insulin no 2-DG treatment. B: 2DG uptake in the absence of insulin. C: 2DG uptake in the presence of 1mM insulin. D: 2DG uptake in the presence of 1mM insulin and 200 mM phloretin. E: 2DG uptake in the presence of insulin 1mM and 5mM D-Glucose.)

Protocol

Catalog	Size	Price	Quantity
36503	100 Tests	Price
36504	500 Tests	Price

Overview

Screen Quest™ Colorimetric Glucose Uptake Assay Kit is designed for measuring glucose uptake in tissues or cultured cells using a non-radioactive colorimetric assay format. Glucose transport systems move glucose across cell membranes, and uptake of 2-deoxyglucose (2-DG), a glucose analog, is widely used to estimate cellular glucose uptake and study the regulation of glucose metabolism.

In this assay, 2-DG is taken up by glucose transporters and converted to 2-deoxyglucose-6-phosphate (2-DG6P), which accumulates inside cells because it is not further metabolized through glycolysis. The accumulated 2-DG6P is enzymatically oxidized to generate NADPH, which is detected using a chromogenic NADPH sensor. The colorimetric signal is measured with an absorbance microplate reader using the OD ratio at 570 nm to 610 nm. Screen Quest™ Colorimetric Glucose Uptake Assay Kit is suitable for glucose uptake analysis, glucose transporter studies, metabolic regulation research, and insulin resistance-related cell or tissue studies.

Components

Catalog 36503 (100 Tests)

Component A: 2-Deoxyglucose (2-DG, 10mM)	1 vial (1 mL)
Component B: Glucose Uptake Buffer	1 bottle (10 mL)
Component C: Acidic Lysis Buffer	1 bottle (2.5 mL)
Component D: Neutralization Buffer	1 bottle (2.5 mL)
Component E: Enzyme Probe	1 bottle (lyophilized powder)
Component F: Assay Buffer	1 bottle (5 mL)
Component G: NADP	1 vial
Component H: 5x KRPH Buffer	1 bottle (20 mL)

Catalog 36504 (500 Tests)

Component A: 2-Deoxyglucose	1 bottle (5 mL)
Component B: Glucose Uptake Buffer	1 bottle (50 mL)
Component C: Acidic Lysis Buffer	1 bottle (12.5 mL)
Component D: Neutralization Buffer	1 bottle (12.5 mL)
Component E: Enzyme Probe	5 bottles (lyophilized powder)
Component F: Assay Buffer	1 bottle (25 mL)
Component G: NADP	5 vials
Component H: 5X KRPH Buffer	1 bottle (100 mL)

Citations

View all 20 citations: Citation Explorer

NAT10-mediated ac4C acetylation of PKM2 promotes papillary thyroid carcinoma through regulating glycolysis

Authors:

Qiu, Guobin and Cen, Yinghuan and Li, Yuan and Ye, Jiaan and Li, Sufen and Li, Xin

Journal:

Journal of Molecular Histology (2026): 265

Peritumoral colonic epithelial cell-derived GDF15 sustains colorectal cancer via regulation of glycolysis and histone lactylation

Authors:

Guan, Bingjie and Zhou, Mantang and Dai, Weixing and Zhang, Jing and Xie, Bowen and Mi, Yushuai and Zhang, Xin and Wei, Ping and Liu, Youdong and Li, Shanbao and others,

Journal:

Nature Aging (2025): 1--17

CUEDC1 promotes glycolytic metabolism reprogramming through the CUEDC1/CACNG4/PI3K axis to promote ER-positive breast cancer growth

Authors:

Lu, Zexiu and Lei, Ming and Chen, Jian and Deng, Ao and Chang, Chao and Chen, Jing and Meng, Die and Wang, Rui and Wan, Xueying and Tu, Gang and others,

Journal:

Cellular \& Molecular Biology Letters (2025): 143

Hypoxia-induced PYCR1 regulates glycolysis and histone lactylation to promote bladder cancer progression and metastasis via SLC6A14/Glutamine metabolism

Authors:

Li, Zhuo and Jiang, Qinghua and Yang, Quan and Zhou, Yujie and Wang, Jiansong

Journal:

Cancer Biology \& Therapy (2025): 2546219

An obesogenic FTO allele causes accelerated development, growth and insulin resistance in human skeletal muscle cells

Authors:

Guang, Lu and Ma, Shilin and Yao, Ziyue and Song, Dan and Chen, Yu and Liu, Shuqing and Wang, Peng and Su, Jiali and Wang, Yuefan and Luo, Lanfang and others,

Journal:

Nature Communications (2025): 1645

References

View all 115 references: Citation Explorer

Inhibition of the sodium glucose co-transporter-2: its beneficial action and potential combination therapy for type 2 diabetes mellitus

Authors:

Chen LH, Leung PS.

Journal:

Diabetes Obes Metab. (2013)

Hepatic expression and cellular distribution of the glucose transporter family

Authors:

Karim S, Adams DH, Lalor PF.

Journal:

World J Gastroenterol (2012): 6771

Sodium glucose co-transporter 2 (SGLT2) inhibitors: novel antidiabetic agents

Authors:

Washburn WN., undefined

Journal:

Expert Opin Ther Pat (2012): 483

Research progress of sodium-glucose co-transporter 2 inhibitors for treatment of type 2 diabetes

Authors:

Wan HX, Shen JK.

Journal:

Yao Xue Xue Bao (2012): 716

A new class of drug for the management of type 2 diabetes: sodium glucose co-transporter inhibitors: 'glucuretics'

Authors:

Chan HW, Ashan B, Jayasekera P, Collier A, Ghosh S.

Journal:

Diabetes Metab Syndr (2012): 224

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	570/610 nm
Recommended plate	Clear bottom

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Page updated on October 6, 2026