Name: Screen Quest™ Colorimetric Glucose Uptake Assay Kit
Brand: AAT Bioquest
SKU: 36503
Availability: InStock

Screen Quest™ Colorimetric Glucose Uptake Assay Kit

Glucose transport systems are responsible for transporting glucose across cell membranes. Measuring uptake of 2-deoxyglucose (2-DG), a glucose analog, in tissues and cells is widely accepted as a reliable method to estimate the amount of glucose uptake and to investigate the regulation of glucose metabolism and mechanism of insulin resistance. The 2-DG uptake is commonly determined by using non-metabolized 2-DG labeled with tritium or C14. However, routine use of a radiolabelled probe is costly and requires a tedious special handling procedure. AAT Bioquest's Screen Quest™ Colorimetric Glucose Uptake Assay Kit provides a sensitive and non-radioactive assay in tissues or cultured cells. In this assay 2-DG is taken up by glucose transporters, and metabolized to 2-DG-6-phosphate (2-DG6P). The non-metabolizable 2-DG6P accumulates in the cells, and is proportional to glucose uptake by cells. The accumulated 2-DG6P is enzymatically oxidized and generates NADPH, which is specifically monitored by a chromogenic NADPH sensor. The signal can be read by a absorption microplate reader by reading the OD ratio at wavelength 570 nm to 610 nm.

Measurement of 2DG uptake in differentiated 3T3-L1 adipocytes and 3T3-L1 fibroblasts. Assays were performed with Screen Quest™ Colorimetric Glucose Uptake Assay Kit in a black wall/clear bottom cell culture Poly-D lysine plate using a SpectraMax (Molecular Devices) microplate reader. (A: Negative Control, no insulin no 2-DG treatment. B: 2DG uptake in the absence of insulin. C: 2DG uptake in the presence of 1mM insulin. D: 2DG uptake in the presence of 1mM insulin and 200 mM phloretin. E: 2DG uptake in the presence of insulin 1mM and 5mM D-Glucose.)

Protocol

COA

Catalog	Size	Price	Quantity
36503	100 Tests	Price
36504	500 Tests	Price

Components

Catalog 36503 (100 Tests)

Component A: 2-Deoxyglucose (2-DG, 10mM)	1 vial (1 mL)
Component B: Glucose Uptake Buffer	1 bottle (10 mL)
Component C: Acidic Lysis Buffer	1 bottle (2.5 mL)
Component D: Neutralization Buffer	1 bottle (2.5 mL)
Component E: Enzyme Probe	1 bottle (lyophilized powder)
Component F: Assay Buffer	1 bottle (5 mL)
Component G: NADP	1 vial
Component H: 5x KRPH Buffer	1 bottle (20 mL)

Catalog 36504 (500 Tests)

Component A: 2-Deoxyglucose (2-DG, 10mM)	1 bottle (5 mL)
Component B: Glucose Uptake Buffer	1 bottle (50 mL)
Component C: Acidic Lysis Buffer	1 bottle (12.5 mL)
Component D: Neutralization Buffer	1 bottle (12.5 mL)
Component E: Enzyme Probe	5 bottles (lyophilized powder)
Component F: Assay Buffer	1 bottle (25 mL)
Component G: NADP	5 vials
Component H: 5x KRPH Buffer	1 bottle (100 mL)

Citations

View all 17 citations: Citation Explorer

Hypoxia-induced PYCR1 regulates glycolysis and histone lactylation to promote bladder cancer progression and metastasis via SLC6A14/Glutamine metabolism

Authors:

Li, Zhuo and Jiang, Qinghua and Yang, Quan and Zhou, Yujie and Wang, Jiansong

Journal:

Cancer Biology \& Therapy (2025): 2546219

An obesogenic FTO allele causes accelerated development, growth and insulin resistance in human skeletal muscle cells

Authors:

Guang, Lu and Ma, Shilin and Yao, Ziyue and Song, Dan and Chen, Yu and Liu, Shuqing and Wang, Peng and Su, Jiali and Wang, Yuefan and Luo, Lanfang and others,

Journal:

Nature Communications (2025): 1645

ETS1 Promotes Aerobic Glycolysis and Growth in Head and Neck Squamous Cell Carcinoma by Targeting RRAS2

Authors:

Liu, Jianguo and Wang, Zhi and Tian, Xiaoyan and Xie, Bingbin and Liu, Ke

Journal:

Biochemical Genetics (2024): 1--17

TRIM33 promotes glycolysis through regulating P53 K48-linked ubiquitination to promote esophageal squamous cell carcinoma growth

Authors:

Xia, Tian and Meng, Lian and Xu, Guixuan and Sun, Hao and Chen, Hao

Journal:

Cell Death \& Disease (2024): 740

Revealing the Mechanism of Esculin in Treating Renal Cell Carcinoma Based on Network Pharmacology and Experimental Validation

Authors:

Chen, Zixuan and Wang, Cunzhou and Cai, Yuesong and Xu, An and Han, Chengtao and Tong, Yanjun and Cheng, Sheng and Liu, Min

Journal:

Biomolecules (2024): 1043

References

View all 115 references: Citation Explorer

Inhibition of the sodium glucose co-transporter-2: its beneficial action and potential combination therapy for type 2 diabetes mellitus

Authors:

Chen LH, Leung PS.

Journal:

Diabetes Obes Metab. (2013)

Hepatic expression and cellular distribution of the glucose transporter family

Authors:

Karim S, Adams DH, Lalor PF.

Journal:

World J Gastroenterol (2012): 6771

Sodium glucose co-transporter 2 (SGLT2) inhibitors: novel antidiabetic agents

Authors:

Washburn WN., undefined

Journal:

Expert Opin Ther Pat (2012): 483

Research progress of sodium-glucose co-transporter 2 inhibitors for treatment of type 2 diabetes

Authors:

Wan HX, Shen JK.

Journal:

Yao Xue Xue Bao (2012): 716

A new class of drug for the management of type 2 diabetes: sodium glucose co-transporter inhibitors: 'glucuretics'

Authors:

Chan HW, Ashan B, Jayasekera P, Collier A, Ghosh S.

Journal:

Diabetes Metab Syndr (2012): 224

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Absorbance microplate reader
Absorbance	570/610 nm
Recommended plate	Clear bottom

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