Screen Quest™ Fluo-4 No Wash Calcium Assay Kit is designed for fluorescence-based detection of intracellular calcium mobilization in live cells. Calcium flux assays are commonly used in drug discovery for screening G protein-coupled receptors (GPCRs) and calcium channels. The kit uses Fluo-4 AM, a cell-permeant calcium indicator that enters cells and is converted by intracellular esterases into the negatively charged Fluo-4 dye retained inside cells.

When cells are stimulated with screening compounds, receptor-mediated calcium release or calcium influx increases the fluorescence of Fluo-4 upon calcium binding. The no-wash homogeneous assay format simplifies calcium flux detection and supports microplate-based workflows in 96-well or 384-well formats that can be adapted to automation. Screen Quest™ Fluo-4 No Wash Calcium Assay Kit is suitable for GPCR calcium signaling studies, calcium channel assays, intracellular calcium mobilization analysis, and high-throughput calcium screening applications.