Name: Screen Quest™ Fluo-8® No Wash Calcium Assay Kit
Brand: AAT Bioquest
SKU: 36314
Availability: InStock

Screen Quest™ Fluo-8® No Wash Calcium Assay Kit

Screen Quest™ Fluo-8® No Wash Calcium Assay Kit is a homogeneous fluorescence-based kit for monitoring intracellular calcium mobilization in cell-based microplate assays.

No-wash format: Enables calcium response monitoring without medium removal or washing under the recommended assay conditions
Calcium-responsive indicator: Cell-permeable Fluo-8® NW undergoes intracellular esterase cleavage, and the resulting indicator increases in fluorescence upon calcium binding
Microplate compatibility: Supports 96-well and 384-well formats and automated fluorescence detection
Research applications: Suitable for screening calcium-coupled GPCRs and evaluating calcium channel–associated responses

Carbachol Dose Response was measured in HEK-293 cells with Screen Quest™ Fluo-8 No Wash Calcium Assay Kit and Fluo-4 NW Calcium Assay Kit. HEK-293 cells were seeded overnight at 40,000 cells/100 µL/well in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 µL of dye-loading solution using the Screen Quest™ Fluo-8 No Wash calcium assay kit or Fluo-4 NW kit (according to the manufacturer's instructions) for 1 hour at room temperature. Carbachol (50µL/well) was added by NOVOstar (BMG Labtech) to achieve the final indicated concentrations.

Protocol

Catalog	Size	Price
36314	1 Plate	Price
36315	10 Plates	Price
36316	100 Plates	Price

Overview

Screen Quest™ Fluo-8® No Wash Calcium Assay Kit detects intracellular calcium mobilization using the calcium-responsive indicator Fluo-8® NW. Following cell entry, intracellular esterases cleave the indicator’s lipophilic blocking groups to generate a charged form that favors intracellular retention. Calcium binding increases fluorescence, allowing changes in intracellular calcium to be monitored following stimulation.

The homogeneous no-wash format supports fluorescence detection without medium removal or washing under the recommended assay conditions. Compatible with 96-well and 384-well microplates and automated detection, the assay uses recommended reader settings of Ex/Em = 490/525 nm. The kit is suitable for high-throughput screening of calcium-coupled GPCRs and functional studies of calcium channels.

Components

Catalog 36314 (1 Plate)

Component A: Fluo-8® NW	1 vial, lyophilized
Component B: 10X Pluronic® F127 Plus	1 bottle (1 mL)
Component C: HHBS (Hanks' buffer with 20 mM Hepes)	1 bottle (9 mL)

Catalog 36315 (10 Plates)

Component A: Fluo-8® NW	1 vial, lyophilized
Component B: 10X Pluronic® F127 Plus	10 bottles (1 mL/bottle)
Component C: HHBS (Hanks' buffer with 20 mM Hepes)	1 bottle (100 mL)

Catalog 36316 (100 Plates)

Component A: Fluo-8® NW	10 vials, lyophilized
Component B: 10X Pluronic® F127 Plus	10 bottles (1 mL/bottle)

Citations

View all 245 citations: Citation Explorer

CO2-rich solution promotes angiogenesis and accelerates wound healing associated with Ca2⁺, AKT and ERK signaling

Authors:

Li, Huan and Kunieda, Sakurako and Ueda, Takayuki and Taketani, Shigeru and Hihara, Masakatsu and Kakudo, Natsuko

Journal:

Scientific Reports (2026)

Selective GPR17 antagonism enhances structural and functional recovery in animal models of demyelination

Authors:

De Herdt, Dille and Lefevere, Evy and Brouwers, V{\'e}ronique and Hartvig, Line and Geeraerts, Emiel and Hsiao, Cheng-Chih and Chen, JQ Alida and Pinto, Rui and Duvey, Guillaume and Burbidge, Stephen and others,

Journal:

PloS one (2026): e0354525

Effects of Fluoride and 8: 2 FTOH on $\beta$-Cell Calcium Signaling and Insulin Homeostasis: An Exploratory Study

Authors:

Trevizol, Juliana Sanches and Okamoto, Motoki and Yamashita, Shohei and Kuriki, Nanako and Brueckner, Susanne and Shindo, Satoru and Kawai, Toshihisa and Dami{\~a}o, Raissa Estefane Vaz and Dionizio, Aline and Buzalaf, Marilia Afonso Rabelo and others,

Journal:

Metabolites (2026): 470

CO2-Rich Solution Activates Ca2+-AKT-ERK Signaling to Promote Angiogenesis and Wound Healing

Authors:

Li, Huan and Kunieda, Sakurako and Ueda, Takayuki and Taketani, Shigeru and Hihara, Masakatsu and Kakudo, Natsuko

Infectious bronchitis virus accessory protein 3a induces renal inflammatory injury by activating the NLRP3 inflammasome via ER calcium mobilization and mitochondrial ROS production

Authors:

Huang, Min and Liukang, Chengyin and Yang, Huiming and Sun, Lu and Tang, Lihua and Lv, Pinyi and Hou, Feixuan and Zhao, Ye and Zhang, Guozhong and Zhao, Jing

Journal:

Journal of Virology (2026): e02125--25

Spectral properties

Correction factor (260 nm)	1.076
Correction factor (280 nm)	0.769
Extinction coefficient (cm ^-1 M ^-1)	23430
Excitation (nm)	495
Emission (nm)	516
Quantum yield	0.16 ¹

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	510 nm
Recommended plate	Black wall/Clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

Other instruments	ArrayScan, FDSS, FLIPR, FlexStation, IN Cell Analyzer, NOVOStar, ViewLux

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 4, 2026