Screen Quest™ Fluorimetric Fatty Acid Uptake Assay Kit monitors cellular fatty acid uptake using TF2-C12, a proprietary fluorescent dodecanoic acid substrate. Cellular uptake of the labeled substrate is assessed through fluorescence measurements, providing a readout for comparing uptake across experimental conditions. The assay supports both kinetic and endpoint detection.

The mix-and-read format supports 96-well and 384-well microplates and can be adapted to high-throughput screening. Detection requires a compatible bottom-read fluorescence microplate reader using recommended settings of Ex/Em = 485/515 nm or an appropriate FITC channel. Compound concentration and exposure time require empirical optimization for the selected cell model, with matched DMSO vehicle controls where relevant. The kit supports research on cellular fatty acid uptake and screening of compounds that alter uptake.