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Screen Quest™ Fluorimetric Fatty Acid Uptake Assay Kit
Fatty acid uptake is an important therapeutic target for the treatment of many human diseases such as obesity, type 2 diabete and hepatic steatosis. The Screen Quest™ Fluorimetric Fatty Acid Uptake Assay Kit provides a simple and sensitive method for the measurement of fatty acid uptake in cells containing fatty acid transporters. The kit uses a proprietary dodecanoic acid fluorescent fatty acid substrate. This fatty acid uptake assay kit can be performed on any fluorescence microplate reader with a bottom-read mode or FITC channel. The assay can be performed in 96-well or 384-well microtiter plates in a simple mix-and-read procedure, and easily adapted for high throughput screening applications.
Comparison of fatty acid uptake by 3T3-L1 adipocytes and fibroblast.&nbsp; Cells were plated at 50,000 cells/100 mL/well in a 96 well black wall/clear bottom poly-D lysine plate for 5 hours, and then serum deprived for 1 hour. Cells were treated without (control) or with insulin (150 nM), and incubated at 37 &ordm;C, 5% CO<sub>2 </sub>incubator for 30 min. At the end of the incubation time, 100 &micro;l of fatty acid mixture was added into the well, and incubated for another 60 min, the fluorescence signal was measured with a FlexStation plate reader using bottom read mode. A &ndash; fibroblasts (Control); B &ndash; fibroblasts (Insulin); C &ndash; adipocytes (Control); D&ndash; adipocytes (Insulin).
Comparison of fatty acid uptake by 3T3-L1 adipocytes and fibroblast.&nbsp; Cells were plated at 50,000 cells/100 mL/well in a 96 well black wall/clear bottom poly-D lysine plate for 5 hours, and then serum deprived for 1 hour. Cells were treated without (control) or with insulin (150 nM), and incubated at 37 &ordm;C, 5% CO<sub>2 </sub>incubator for 30 min. At the end of the incubation time, 100 &micro;l of fatty acid mixture was added into the well, and incubated for another 60 min, the fluorescence signal was measured with a FlexStation plate reader using bottom read mode. A &ndash; fibroblasts (Control); B &ndash; fibroblasts (Insulin); C &ndash; adipocytes (Control); D&ndash; adipocytes (Insulin).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
36385100 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation485 nm
Emission515 nm
Cutoff495 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on September 22, 2025