Screen Quest™ Fluorimetric Glucose Uptake Assay Kit

Screen Quest™ Fluorimetric Glucose Uptake Assay Kit is a non-radioactive fluorescence-based kit for assessing cellular glucose uptake through detection of accumulated 2-deoxyglucose-6-phosphate.

Glucose analog uptake: Uses 2-deoxyglucose to assess cellular uptake through detection of its intracellular phosphorylated product
Enzyme-coupled detection: Enzymatic oxidation of accumulated 2-deoxyglucose-6-phosphate generates NADPH for fluorogenic detection
Non-radioactive readout: Measures fluorescence in cell lysates using a fluorescence microplate reader
Research applications: Suitable for studying insulin-regulated glucose uptake and evaluating compounds that modulate cellular glucose uptake

Measurement of 2DG uptake in differentiated 3T3-L1 adipocytes and 3T3-L1 fibroblasts. Assays were performed with Screen Quest™ Fluorimetric Glucose Uptake Assay Kit in a black wall/clear bottom cell culture Poly-D lysine plate using a Gemini (Molecular Devices) microplate reader. (A: Negative Control, no insulin no 2-DG treatment. B: 2DG uptake in the absence of insulin. C: 2DG uptake in the presence of 1mM insulin. D: 2DG uptake in the presence of 1mM insulin and 200 mM phloretin. E: 2DG uptake in the presence of insulin 1mM and 5mM D-Glucose.)

Protocol

Catalog	Size	Price	Quantity
36500	100 Tests	Price
36501	500 Tests	Price

Overview

Screen Quest™ Fluorimetric Glucose Uptake Assay Kit assesses cellular glucose uptake using the glucose analog 2-deoxyglucose (2-DG). Following transport into cells, 2-DG is phosphorylated to 2-deoxyglucose-6-phosphate (2-DG6P), which accumulates intracellularly. In the detection reaction, accumulated 2-DG6P is enzymatically oxidized to generate NADPH, which is measured using a fluorogenic NADPH sensor.

The assay provides a non-radioactive fluorescence readout in cell lysates, with recommended reader settings of Ex/Em = 540/590 nm. Because the readout depends on both cellular uptake and intracellular phosphorylation of 2-DG, it serves as a proxy for glucose uptake. Compound concentration and exposure time require empirical optimization for the selected cell model. The kit supports studies of insulin-regulated glucose uptake, insulin resistance, and compound effects on cellular glucose uptake.

Components

Catalog 36500 (100 Tests)

Component A: 2-Deoxyglucose (2-DG, 10mM )	1 vial (1 mL)
Component B: Glucose Uptake Buffer	1 bottle (10 mL)
Component C: Acidic Lysis Buffer	1 bottle (2.5 mL)
Component D: Neutralization Buffer	1 bottle (2.5 mL)
Component E: Enzyme Probe	1 bottle (lyophilized powder)
Component F: Assay Buffer	1 bottle (5 mL)
Component G: NADP	1 vial
Component H: 5x KRPH Buffer	1 bottle (20 mL)

Catalog 36501 (500 Tests)

Component A: 2-Deoxyglucose	1 bottle (5 mL)
Component B: Glucose Uptake Buffer	1 bottle (50 mL)
Component C: Acidic Lysis Buffer	1 bottle (12.5 mL)
Component D: Neutralization Buffer	1 bottle (12.5 mL)
Component E: Enzyme Probe	5 bottles (lyophilized powder)
Component F: Assay Buffer	1 bottle (25 mL)
Component G: NADP	5 vials
Component H: 5x KRPH Buffer	1 bottle (100 mL)

Citations

View all 15 citations: Citation Explorer

Twist1 protects intervertebral disc from degeneration via GLUT3 mediated glycolysis

Authors:

Zhang, Yizhi and Wang, Yifan and Zhou, Hongyu and Li, Lei and Zhong, Huajian and Gao, Yang and Shen, Xiaolong

Journal:

Pathology-Research and Practice (2026): 156686

Peritumoral colonic epithelial cell-derived GDF15 sustains colorectal cancer via regulation of glycolysis and histone lactylation

Authors:

Guan, Bingjie and Zhou, Mantang and Dai, Weixing and Zhang, Jing and Xie, Bowen and Mi, Yushuai and Zhang, Xin and Wei, Ping and Liu, Youdong and Li, Shanbao and others,

Journal:

Nature Aging (2025): 1--17

YULINK deficiency promotes cell death under glucose restriction in HCC cells in association with GLUT1-mediated glycolysis

Authors:

Wu, Yi-Chia and Hung, Tsai-Hsien and Wang, Wei-Ting Thomas and Kuo, Ming-Wei and Liang, Yuh-Jin and Kuo, Yur-Ren and Hou, Ming-Feng and Lai, Chung-Sheng and Yu, Alice L and Yu, John

Journal:

Molecular Medicine (2025): 1--21

ETS1 Promotes Aerobic Glycolysis and Growth in Head and Neck Squamous Cell Carcinoma by Targeting RRAS2

Authors:

Liu, Jianguo and Wang, Zhi and Tian, Xiaoyan and Xie, Bingbin and Liu, Ke

Journal:

Biochemical Genetics (2024): 1--17

miR-486-5p-rich extracellular vesicles derived from patients with olanzapine-induced insulin resistance negatively affect glucose-regulating function

Authors:

Tu, Chuyue and Wu, Qian and Wang, Jing and Chen, Peiru and Deng, Yahui and Yu, Lixiu and Xu, Xiaojin and Fang, Xiangming and Li, Weiyong

Journal:

Biochemical Pharmacology (2024): 116308

References

View all 115 references: Citation Explorer

Inhibition of the sodium glucose co-transporter-2: its beneficial action and potential combination therapy for type 2 diabetes mellitus

Authors:

Chen LH, Leung PS.

Journal:

Diabetes Obes Metab. (2013)

Hepatic expression and cellular distribution of the glucose transporter family

Authors:

Karim S, Adams DH, Lalor PF.

Journal:

World J Gastroenterol (2012): 6771

Sodium glucose co-transporter 2 (SGLT2) inhibitors: novel antidiabetic agents

Authors:

Washburn WN., undefined

Journal:

Expert Opin Ther Pat (2012): 483

Research progress of sodium-glucose co-transporter 2 inhibitors for treatment of type 2 diabetes

Authors:

Wan HX, Shen JK.

Journal:

Yao Xue Xue Bao (2012): 716

A new class of drug for the management of type 2 diabetes: sodium glucose co-transporter inhibitors: 'glucuretics'

Authors:

Chan HW, Ashan B, Jayasekera P, Collier A, Ghosh S.

Journal:

Diabetes Metab Syndr (2012): 224

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 10, 2026