Screen Quest™ Fluorimetric Glucose Uptake Assay Kit

Glucose transport systems are responsible for transporting glucose across cell membranes. Measuring uptake of 2-deoxyglucose (2-DG), a glucose analog, in tissues and cells is widely accepted as a reliable method to estimate the amount of glucose uptake and to investigate the regulation of glucose metabolism and mechanism of insulin resistance. The 2-DG uptake is commonly determined by using non-metabolized 2-DG labeled with tritium or C14. However, routine use of a radiolabelled probe is costly and requires a tedious special handling procedure. AAT Bioquest's Screen Quest™ Fluorimetric Glucose Uptake Assay Kit provides a sensitive and non-radioactive assay in cells. In this assay 2-DG is taken up by glucose transporters, and metabolized to 2-DG-6-phosphate (2-DG6P). The non-metabolizable 2-DG6P accumulates in the cells, and is proportional to glucose uptake by cells. The accumulated 2-DG6P is enzymatically oxidized and generates NADPH, which is specifically monitored by a fluorogenic NADPH sensor. The signal can be read by a fluorescence microplate reader.

Measurement of 2DG uptake in differentiated 3T3-L1 adipocytes and 3T3-L1 fibroblasts. Assays were performed with Screen Quest™ Fluorimetric Glucose Uptake Assay Kit in a black wall/clear bottom cell culture Poly-D lysine plate using a Gemini (Molecular Devices) microplate reader. (A: Negative Control, no insulin no 2-DG treatment. B: 2DG uptake in the absence of insulin. C: 2DG uptake in the presence of 1mM insulin. D: 2DG uptake in the presence of 1mM insulin and 200 mM phloretin. E: 2DG uptake in the presence of insulin 1mM and 5mM D-Glucose.)

Protocol

Catalog	Size	Price	Quantity
36500	100 Tests	Price
36501	500 Tests	Price

Components

Catalog 36500 (100 Tests)

Component A: 2-Deoxyglucose (2-DG, 10mM )	1 vial (1 mL)
Component B: Glucose Uptake Buffer	1 bottle (10 mL)
Component C: Acidic Lysis Buffer	1 bottle (2.5 mL)
Component D: Neutralization Buffer	1 bottle (2.5 mL)
Component E: Enzyme Probe	1 bottle (lyophilized powder)
Component F: Assay Buffer	1 bottle (5 mL)
Component G: NADP	1 vial
Component H: 5x KRPH Buffer	1 bottle (20 mL)

Catalog 36501 (500 Tests)

Component A: 2-Deoxyglucose	1 bottle (5 mL)
Component B: Glucose Uptake Buffer	1 bottle (50 mL)
Component C: Acidic Lysis Buffer	1 bottle (12.5 mL)
Component D: Neutralization Buffer	1 bottle (12.5 mL)
Component E: Enzyme Probe	5 bottles (lyophilized powder)
Component F: Assay Buffer	1 bottle (25 mL)
Component G: NADP	5 vials
Component H: 5x KRPH Buffer	1 bottle (100 mL)

Citations

View all 14 citations: Citation Explorer

Peritumoral colonic epithelial cell-derived GDF15 sustains colorectal cancer via regulation of glycolysis and histone lactylation

Authors:

Guan, Bingjie and Zhou, Mantang and Dai, Weixing and Zhang, Jing and Xie, Bowen and Mi, Yushuai and Zhang, Xin and Wei, Ping and Liu, Youdong and Li, Shanbao and others,

Journal:

Nature Aging (2025): 1--17

YULINK deficiency promotes cell death under glucose restriction in HCC cells in association with GLUT1-mediated glycolysis

Authors:

Wu, Yi-Chia and Hung, Tsai-Hsien and Wang, Wei-Ting Thomas and Kuo, Ming-Wei and Liang, Yuh-Jin and Kuo, Yur-Ren and Hou, Ming-Feng and Lai, Chung-Sheng and Yu, Alice L and Yu, John

Journal:

Molecular Medicine (2025): 1--21

ETS1 Promotes Aerobic Glycolysis and Growth in Head and Neck Squamous Cell Carcinoma by Targeting RRAS2

Authors:

Liu, Jianguo and Wang, Zhi and Tian, Xiaoyan and Xie, Bingbin and Liu, Ke

Journal:

Biochemical Genetics (2024): 1--17

miR-486-5p-rich extracellular vesicles derived from patients with olanzapine-induced insulin resistance negatively affect glucose-regulating function

Authors:

Tu, Chuyue and Wu, Qian and Wang, Jing and Chen, Peiru and Deng, Yahui and Yu, Lixiu and Xu, Xiaojin and Fang, Xiangming and Li, Weiyong

Journal:

Biochemical Pharmacology (2024): 116308

TFAIP6 facilitates hepatocellular carcinoma cell glycolysis through upregulating c-Myc/PKM2 axis

Authors:

Duan, Kecai and Fang, Kunpeng and Sui, Chengjun

Journal:

Heliyon (2024): e30959

References

View all 115 references: Citation Explorer

Inhibition of the sodium glucose co-transporter-2: its beneficial action and potential combination therapy for type 2 diabetes mellitus

Authors:

Chen LH, Leung PS.

Journal:

Diabetes Obes Metab. (2013)

Hepatic expression and cellular distribution of the glucose transporter family

Authors:

Karim S, Adams DH, Lalor PF.

Journal:

World J Gastroenterol (2012): 6771

Regulation of glucose transporter translocation in health and diabetes

Authors:

Bogan JS., undefined

Journal:

Annu Rev Biochem (2012): 507

New possibility in the oral glucose lowering treatment of type 2 diabetes mellitus: sodium-glucose co-transporter-2 inhibitors

Authors:

Simonyi G., undefined

Journal:

Orv Hetil (2012): 695

Glucose transporter type I deficiency syndrome: epilepsy phenotypes and outcomes

Authors:

Pong AW, Geary BR, Engelstad KM, Natarajan A, Yang H, De Vivo DC.

Journal:

Epilepsia (2012): 1503

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on July 15, 2026