Screen Quest™ Luminometric Calcium Assay Kit

Screen Quest™ Luminometric Calcium Assay Kit is a bioluminescence-based kit for monitoring intracellular calcium changes in apoaequorin-expressing cells using a membrane-permeable coelenterazine analog.

Aequorin-based detection: Provides a coelenterazine analog that combines with expressed apoaequorin to form a calcium-responsive photoprotein
Luminescent readout: Calcium binding triggers light emission without external excitation
Microplate compatibility: Supports 96-well and 384-well formats and adaptation to automated luminescence detection
Research applications: Suitable for screening calcium-coupled GPCRs and evaluating calcium channel–associated responses in apoaequorin-expressing cells

ATP Dose Response on CHO-aeq cells. CHO cells stably transfected with apoaequrin were seeded overnight at 50,000 cells/100 µL/well in a Costar white wall/clear bottom 96-well plate. The growth medium was removed and the cells were incubated with 100 µL of dye-loading solution using the Screen Quest™ Coelenterazine Calcium Assay Kit for 3 hours at room temperature and protected from light. ATP (25 µL/well) was added by NOVOstar (BMG Labtech) to achieve the final indicated concentrations. The EC50 of ATP is about 0.8 µM.

Protocol

Catalog	Size	Price	Quantity
36305	10 Plates	Price
36306	100 Plates	Price

Overview

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). This kit uses a highly calcium-sensitive and membrane-permeable coelenterazine analog as a calcium indicator for the cells that are transfected with apoaequorin gene. Aequorin is a calcium-sensitive bioluminescent protein from the jellyfish Aequorea victoria that has been used extensively as a calcium indicator in cells. The aequorin complex emits blue light when bound to calcium ions. The luminescence intensity is directly proportional to the Ca2+ concentration. Our coelenterazine-based kit is much more sensitive than the fluorescence-based calcium assay kits (such as Fluo-4, Fluo-3, Calcium-3 and Calcium-4). This kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. It might be useful for monitoring of intracellular calcium mobilization in a specified compartment given that recombinant apoaequorin proteins can now be targeted to specific organelles, cells and tissues.

Citations

View all 4 citations: Citation Explorer

Sotagliflozin ameliorates doxorubicin-induced cardiomyopathy by regulating inflammation and mitochondrial dysfunction through the AMPK/mTOR pathway

Authors:

Liu, Haifeng and Han, Danye and Li, Meiling

Journal:

Experimental and Therapeutic Medicine (2026): 1--18

Intracellular Ca2+ is important for flagellin-triggered defense in Arabidopsis and involves inositol polyphosphate signaling

Authors:

Ma, Yi and Zhao, Yichen and Berkowitz, Gerald A

Journal:

Journal of Experimental Botany (2017)

Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells

Authors:

Harikumar, Kaleeckal G and Yan, Yan and Xu, Ting-Hai and Melcher, Karsten and Xu, H Eric and Miller, Laurence J

Orchestration of salivary secretion mediated by two different dopamine receptors in the blacklegged tick Ixodes scapularis

Authors:

Kim, Donghun and Simo, Ladislav and Park, Yoonseong

Journal:

Journal of Experimental Biology (2014): 3656--3663

References

View all 150 references: Citation Explorer

Expression, purification and characterization of calcium-triggered luciferin-binding protein of Renilla reniformis

Authors:

Inouye S., undefined

Journal:

Protein Expr Purif. (2006)

Mitochondrial adaptations within chronically ischemic swine myocardium

Authors:

McFalls EO, Sluiter W, Schoonderwoerd K, Manintveld OC, Lamers JM, Bezstarosti K, van Beusekom HM, Sikora J, Ward HB, Merkus D, Duncker DJ.

Journal:

J Mol Cell Cardiol (2006): 980

Blue fluorescent protein from the calcium-sensitive photoprotein aequorin: catalytic properties for the oxidation of coelenterazine as an oxygenase

Authors:

Inouye S, Sasaki S.

Journal:

FEBS Lett (2006): 1977

Self-illuminating quantum dots light the way

Authors:

Frangioni JV., undefined

Journal:

Nat Biotechnol (2006): 326

Transient expression of apoaequorin in zebrafish embryos: extending the ability to image calcium transients during later stages of development

Authors:

Cheung CY, Webb SE, Meng A, Miller AL.

Journal:

Int J Dev Biol (2006): 561

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Luminescence microplate reader
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

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Page updated on October 3, 2026

Component A: Coelenterazine Analog	1 vial, lyophilized
Component B: 100% ETOH	1 vial (500 µL)
Component C: Assay Buffer	1 bottle (100 mL-1X ready to use)

Component A: Coelenterazine Analog	10 vials, lyophilized
Component B: 100% ETOH	1 bottle (5 mL)
Component C: Assay Buffer	1 bottle (100 mL-10X)