Name: Screen Quest™ Membrane Potential Assay Kit *Red Fluorescence*
Brand: AAT Bioquest
SKU: 36004
Availability: InStock

Screen Quest™ Membrane Potential Assay Kit

Red Fluorescence

Screen Quest™ Membrane Potential Assay Kit is a homogeneous fluorescence-based kit for monitoring cellular membrane potential changes associated with ion channel activity.

Membrane potential detection: Uses a proprietary fluorescent indicator to monitor changes in cellular membrane potential
Red fluorescence readout: Supports detection with recommended fluorescence reader settings of Ex/Em = 620/650 nm
Homogeneous microplate format: Supports 96-well and 384-well assays without washing after dye loading and can be adapted to automation
Research applications: Suitable for functional ion channel studies and screening compounds that alter cellular membrane potential

ATP dose response in HEK cells transiently transfected with P2X receptor. HEK cells transiently transfected with P2X receptor were seeded overnight at 40,000 cells/100 µL/well in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 µL of the MP dye-loading solution in a 5% COâ‚‚, 37°C incubator for 60 minutes. ATP (50 µL/well) was added by FlexStation to achieve the final indicated concentrations. The fluorescence signal was measured with bottom read mode at Ex/Em = 620/650 nm (cutoff at 630 nm).

Protocol

Catalog	Size	Price
36004	1 plate	Price
36005	10 plates	Price
36006	100 plates	Price

Overview

Membrane potential is the difference in voltage between the interior and exterior of a cell. The membrane potential allows a cell to function as a battery, providing power to operate a variety of "molecular devices" embedded in the membrane. In electrically excitable cells such as neurons, membrane potential is used for transmitting signals between different parts of a cell. Opening or closing of ion channels at one point in the membrane produces a local change in the membrane potential, which causes electric current to flow rapidly to other points in the membrane. Ion channels have been identified as important drug discovery targets. Our Screen Quest™ Membrane Potential Assay Kit is a homogeneous assay with fast read time. It uses our proprietary long wavelength membrane potential indicator to detect the membrane potential change that is caused by the opening and closing of the ion channels. The red fluorescence of the membrane potential indicator used in the kit has enhanced fluorescence upon entering cells and minimizes the interferences resulted from the screening compounds and/or cellular autofluorescence.

Citations

View all 2 citations: Citation Explorer

A High-Throughput Biosensing Approach for Rapid Screening of Compounds Targeting the hNav1. 1 Channel: Marine Toxins as a Case Study

Authors:

Shen, Huijing and Cui, Yuxia and Liang, Shiyuan and Zhou, Shuang and Li, Yingji and Wu, Yongning and Song, Junxian

Journal:

Marine Drugs (2025): 119

The short-chain fatty acid propionate increases glucagon and FABP4 production, impairing insulin action in mice and humans

Authors:

Tirosh, Amir and Calay, Ediz S and Tuncman, Gurol and Claiborn, Kathryn C and Inouye, Karen E and Eguchi, Kosei and Alcala, Michael and Rathaus, Moran and Holl, undefined and er, Kenneth S and Ron, Idit and others, undefined

Journal:

Science translational medicine (2019): eaav0120

References

View all 25 references: Citation Explorer

A novel high-throughput screening assay for HCN channel blocker using membrane potential-sensitive dye and FLIPR

Authors:

Vasilyev DV, Shan QJ, Lee YT, Soloveva V, Nawoschik SP, Kaftan EJ, Dunlop J, Mayer SC, Bowlby MR.

Journal:

J Biomol Screen (2009): 1119

A high-capacity membrane potential FRET-based assay for the sodium-coupled glucose co-transporter SGLT1

Authors:

Weinglass AB, Swensen AM, Liu J, Schmalhofer W, Thomas A, Williams B, Ross L, Hashizume K, Kohler M, Kaczorowski GJ, Garcia ML.

Journal:

Assay Drug Dev Technol (2008): 255

Miniaturization and HTS of a FRET-based membrane potential assay for K(ir) channel inhibitors

Authors:

Solly K, Cassaday J, Felix JP, Garcia ML, Ferrer M, Strulovici B, Kiss L.

Journal:

Assay Drug Dev Technol (2008): 225

A quantitative evaluation of peroxidase inhibitors for tyramide signal amplification mediated cytochemistry and histochemistry

Authors:

Liu G, Amin S, Okuhama NN, Liao G, Mingle LA.

Journal:

Histochem Cell Biol (2006): 283

Validation of a fluorescent imaging plate reader membrane potential assay for high-throughput screening of glycine transporter modulators

Authors:

Benjamin ER, Skelton J, Hanway D, Olanrewaju S, Pruthi F, Ilyin VI, Lavery D, Victory SF, Valenzano KJ.

Journal:

J Biomol Screen (2005): 365

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	620 nm
Emission	650 nm
Cutoff	630 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

Other instruments	FDSS, FlexStation, NOVOStar

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 7, 2026

Component A: 10X MP Sensor	1 bottle (1 mL)
Component B: HHBS (Hanks' buffer with 20 mM Hepes)	1 bottle (9 mL)

Component A: 10X MP Sensor	1 bottle (10 mL/bottle)
Component B: HHBS (Hanks' buffer with 20 mM Hepes)	1 bottle (100 mL)

Component A: 10X MP Sensor	10 bottles (10 mL/bottle)
Component B: HHBS (Hanks' buffer with 20 mM Hepes)	Not included