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Screen Quest™ Membrane Potential Assay Kit
Red Fluorescence
Membrane potential is the difference in voltage between the interior and exterior of a cell. The membrane potential allows a cell to function as a battery, providing power to operate a variety of "molecular devices" embedded in the membrane. In electrically excitable cells such as neurons, membrane potential is used for transmitting signals between different parts of a cell. Opening or closing of ion channels at one point in the membrane produces a local change in the membrane potential, which causes electric current to flow rapidly to other points in the membrane. Ion channels have been identified as important drug discovery targets. Our Screen Quest™ Membrane Potential Assay Kit is a homogeneous assay with fast read time. It uses our proprietary long wavelength membrane potential indicator to detect the membrane potential change that is caused by the opening and closing of the ion channels. The red fluorescence of the membrane potential indicator used in the kit has enhanced fluorescence upon entering cells and minimizes the interferences resulted from the screening compounds and/or cellular autofluorescence.
ATP dose response in HEK cells transiently transfected with P2X receptor. HEK cells transiently transfected with P2X receptor were seeded overnight at 40,000 cells/100 µL/well in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 µL of the MP dye-loading solution in a 5% COâ‚‚, 37°C incubator for 60 minutes. ATP (50 µL/well) was added by FlexStation to achieve the final indicated concentrations. The fluorescence signal was measured with bottom read mode at Ex/Em = 620/650 nm (cutoff at 630 nm).
ATP dose response in HEK cells transiently transfected with P2X receptor. HEK cells transiently transfected with P2X receptor were seeded overnight at 40,000 cells/100 µL/well in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 µL of the MP dye-loading solution in a 5% COâ‚‚, 37°C incubator for 60 minutes. ATP (50 µL/well) was added by FlexStation to achieve the final indicated concentrations. The fluorescence signal was measured with bottom read mode at Ex/Em = 620/650 nm (cutoff at 630 nm).
protocol
Protocol
COA
CatalogSize
Price
Quantity
360041 plate
Price
3600510 plates
Price
36006100 plates
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation620 nm
Emission650 nm
Cutoff630 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode/Programmable liquid handling
Other instrumentsFDSS, FlexStation, NOVOStar
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Page updated on September 25, 2025