SeneTrace™ Blue is a biotinylated, lipophilic analog of Sudan Black B, engineered for the selective detection of lipofuscin—an autofluorescent, non-degradable lysosomal pigment that accumulates as a consequence of oxidative stress, impaired autophagic flux, and cellular senescence. The biotin moiety facilitates high-affinity detection via anti-biotin antibody-based assays, enabling robust quantification and spatial visualization of lipofuscin aggregates across diverse biological matrices.

Designed for both in vivo and in vitro applications, SeneTrace™ Blue is compatible with a wide range of sample types, including live and fixed cultured cells, fresh and cryopreserved tissues, as well as formalin-fixed, paraffin-embedded (FFPE) specimens. The reagent integrates seamlessly into immunohistochemistry (IHC), flow cytometry, and immunofluorescence (IF) workflows, making it an invaluable tool for investigating cellular aging, neurodegenerative disorders, and age-associated pathologies.

SeneTrace™ Blue is a valuable tool for evaluating lipofuscin accumulation in Vero E6 cells following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and in primary non-small cell lung carcinoma (NSCLC) specimens. Furthermore, it enables the quantification of soluble lipofuscin in human serum, providing mechanistic insights into oxidative damage, chronic inflammation, and age-related disorders, such as dementia.