Signal Guard™ HRP conjugate stabilizer

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Unit Size: Cat No: Price (USD): Qty:
50 mL 11010 $95


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Additional Ordering Information
Telephone: 1-800-990-8053
Fax: 1-408-733-1304
Email: sales@aatbio.com
International: See distributors





Overview

Storage F/D/L
Category Enzyme Detection
Horseradish Peroxidase (HRP)
Related Secondary Reagents
The major advantage of a liquid HRP-conjugate is that it eliminates the chance for human error when dissolving or diluting the lyophilized or concentrated conjugate. Adding too much or not enough buffer results in assay to assay variation. Until now, the disadvantage of this format was the inherent instability of the liquid HRP-conjugates. Our HRP-conjugate stabilizer is formulated to allow you to provide pre-diluted, ready-to-use conjugates. This formulation can be used to create stock solutions (1:1,000) or ready-to-use assay reagents (1:100,000) that can be stored at 4°C for 24 monthss or at room temperature for six months.




Protocol


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This protocol only provides a guideline, and should be modified according to your specific needs.

Note 1: Use buffers, such as Hepes, Tris, Tricine, Mops, PIPES or MES, that contain < 10% phosphate with this HRP Conjugate Stabilizer. Buffers containing > 10% phosphate or significant amounts of borate and carbonate may cause precipitation.

  • Dilute the primary or secondary peroxidase conjugated antibody or other protein or compound with the HRP Conjugate Stabilizer to the desired 1X working concentration determined for your conjugate.
  • This HRP conjugate stabilizer dilutes and stabilizes HRP conjugates in a single step.
  • Do not freeze the HRP conjugates once the HRP conjugate stabilizer is added. Many HRP-conjugates prepared in this manner maintain HRP activity for up to 12 months or longer when properly stored.





References & Citations

Inactivation of creatine kinase induced by quercetin with horseradish peroxidase and hydrogen peroxide. pro-oxidative and anti-oxidative actions of quercetin
Authors: Miura T, Muraoka S, Fujimoto Y.
Journal: Food Chem Toxicol (2003): 759

Mechanistic and molecular investigations on stabilization of horseradish peroxidase C
Authors: Schmidt A, Schumacher JT, Reichelt J, Hecht HJ, Bilitewski U.
Journal: Anal Chem (2002): 3037

Electron microscopical demonstration of horseradish peroxidase by use of tetramethylbenzidine as chromogen and sodium tungstate as stabilizer (TMB-ST method): a tracing method with high sensitivity and well preserved ultrastructural tissue
Authors: Gu Y, Chen Y, Ye L.
Journal: J Neurosci Methods (1992): 1

An ultrastructural double-labelling method: immunohistochemical localization of cell adhesion molecule L1 on HRP-labelled developing corticospinal tract axons in the rat
Authors: Joosten EA.
Journal: Histochemistry (1990): 645

The histochemical reaction of horseradish peroxidase in rodent 'whole-brain' preparations
Authors: Cooper AM.
Journal: Exp Brain Res (1990): 456

A triple-labeling method: HRP anterograde tract tracing combined with double immunofluorescent cell staining in developing neural tissue of the rat
Authors: Dederen PJ, Joosten EA.
Journal: J Neurosci Methods (1989): 71

Improvement of the tetramethyl benzidine reaction with ammonium molybdate as a stabilizer for light and electron microscopic ligand-HRP neurohistochemistry, immunocytochemistry and double-labelling
Authors: Liang FY, Wan XC.
Journal: J Neurosci Methods (1989): 155

[Ammonium molybdate as stabilizing agent in tetramethyl benzidine reaction of CB-HRP neurohistochemistry and its application to electron microscopy and immunocytochemistry]
Authors: Liang FY, Wan XC.
Journal: Zhongguo Yi Xue Ke Xue Yuan Xue Bao (1989): 142

A new stabilizing agent for the tetramethyl benzidine (TMB) reaction product in the histochemical detection of horseradish peroxidase (HRP)
Authors: Olucha F, Martinez-Garcia F, Lopez-Garcia C.
Journal: J Neurosci Methods (1985): 131






Additional Documents

 
Safety Data Sheet (SDS)


Catalogs
1. Enzyme Probes & Assay Kits
2. Reactive Oxygen Species (ROS) Detection

Certificate of Analysis