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Signal Guard™ HRP reaction stopping reagent
HRP coupling reactions provide sensitive biomolecular assays based on hydrogen peroxide- generating enzyme systems linked to peroxidase- mediated oxidation. Fluorogenic HRP substrates are preferred to use for enhancing assay sensitivities. Among them, the most commonly used HRP substrates include ADHP (also called Amplex® Red, #11000), Amplex® UltraRed and Amplite® Red. Typically, detection reactions are performed in microplate wells and are initiated by adding a fluorogenic HRP substrate, resulting in a continuous fluorescence increase. It is critical to ensure that the timing of the standard and unknown sample measurements is the same. Our Signal Guard™ HRP reaction stopping reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable. The Signal Guard™ HRP reaction stopping reagent is designed for use in conjunction with ADHP (Amplex® Red ), Amplite® and Amplex®UltraRed fluorogenic substrates. Under the same conditions, Signal Guard™ HRP reaction stopping reagent significantly outperforms the Amplex® Red/UltraRed Stop Reagent (#A33855) from ThermoFisher. Our Signal Guard™ HRP reaction stopping reagent can also be used in other HRP reaction systems.
Application of HRP Reaction Stop Solution on HRP coupled glucose detection reaction. Two parallel reactions containing 15 µM Glucose were initiated by adding 50 µL assay mixture containing: 0.5mU/mL HRP, Amplex® Red, and 0.5mU/ml Glucose Oxidase. Reactions were incubated at room temperature for 5 mins and then 20 µL 1X Stop Reagent was added to one reaction, and 20 µL dd H2O to the other reaction. The plots demonstrated that the reaction is completely inhibited by Signal Guard™ HRP Reaction Stopping Solution.
Application of HRP Reaction Stop Solution on HRP coupled glucose detection reaction. Two parallel reactions containing 15 µM Glucose were initiated by adding 50 µL assay mixture containing: 0.5mU/mL HRP, Amplex® Red, and 0.5mU/ml Glucose Oxidase. Reactions were incubated at room temperature for 5 mins and then 20 µL 1X Stop Reagent was added to one reaction, and 20 µL dd H2O to the other reaction. The plots demonstrated that the reaction is completely inhibited by Signal Guard™ HRP Reaction Stopping Solution.
protocol
Protocol
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SDS
COO
COA
CatalogSize
Price
Quantity
11020500 Tests
Price
 
Physical properties
SolventWater
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 13, 2025