Signal Guard™ HRP reaction stopping reagent

HRP coupling reactions provide sensitive biomolecular assays based on hydrogen peroxide- generating enzyme systems linked to peroxidase- mediated oxidation. Fluorogenic HRP substrates are preferred to use for enhancing assay sensitivities. Among them, the most commonly used HRP substrates include ADHP (also called Amplex® Red, #11000), Amplex® UltraRed and Amplite® Red. Typically, detection reactions are performed in microplate wells and are initiated by adding a fluorogenic HRP substrate, resulting in a continuous fluorescence increase. It is critical to ensure that the timing of the standard and unknown sample measurements is the same. Our Signal Guard™ HRP reaction stopping reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable. The Signal Guard™ HRP reaction stopping reagent is designed for use in conjunction with ADHP (Amplex® Red ), Amplite® and Amplex®UltraRed fluorogenic substrates. Under the same conditions, Signal Guard™ HRP reaction stopping reagent significantly outperforms the Amplex® Red/UltraRed Stop Reagent (#A33855) from ThermoFisher. Our Signal Guard™ HRP reaction stopping reagent can also be used in other HRP reaction systems.