Signal Guard™ phosphatase reaction stopping solution

Alkaline and acid phosphatases are the hydrolase enzymes responsible for dephosphorylation of molecules such as nucleotides, proteins and alkaloids under alkaline and acidic conditions, respectively. Some phosphatases assays and tests require the phosphatase reactions to be stopped for further analysis using the stop solutions. Our Signal Guard™ phosphatase reaction stopping solution is a ready to use reagent that provides a convenient tool for terminating fluorescence and colorimetric signal-generating phosphatase reactions at a user-determined time point, and also keep the fluorescence or colorimetric signal stable for up to 18 hours. The Signal Guard™ phosphatase reaction stopping solution is optimized and compatible with alkaline, acid and protein phosphatase. Compared to other commercial phosphatase stopping reagents, our Signal Guard™ phosphatases reaction stopping solution is nearly neutral and mild. It is compatible with a vast majority of colorimetric and fluorimetric phosphatase substrates while the stop reagents from other vendors are not compatible with most of fluorescence-based phosphatase assays due to their extremely high pH.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11622	100 mL	Price

Citations

View all 104 citations: Citation Explorer

Discoveries of the phosphatidate phosphatase genes in yeast published in the Journal of Biological Chemistry

Authors:

Carman, G. M.

Journal:

J Biol Chem (2019): 1681-1689

The multiple functions of protein phosphatase 6

Authors:

Ohama, T.

Journal:

Biochim Biophys Acta Mol Cell Res (2019): 74-82

Tuning the Protein Phosphorylation by Receptor Type Protein Tyrosine Phosphatase Epsilon (PTPRE) in Normal and Cancer Cells

Authors:

Liang, J., Shi, J., Wang, N., Zhao, H., Sun, J.

Journal:

J Cancer (2019): 105-111

Dynamic structural rearrangements and functional regulation of voltage-sensing phosphatase

Authors:

Sakata, S., Okamura, Y.

Journal:

J Physiol (2019): 29-40

Redox inhibition of protein phosphatase PP2A: Potential implications in oncogenesis and its progression

Authors:

Raman, D., Pervaiz, S.

Journal:

Redox Biol (2019): 101105

References

View all 33 references: Citation Explorer

Enzyme-nanoparticle functionalization of three-dimensional protein scaffolds

Authors:

Hill RT, Shear JB.

Journal:

Anal Chem (2006): 7022

TNP-8N3-ADP photoaffinity labeling of two Na,K-ATPase sequences under separate Na+ plus K+ control

Authors:

Ward DG, Taylor M, Lilley KS, Cavieres JD.

Journal:

Biochemistry (2006): 3460

Xenopus apyrase (xapy), a secreted nucleotidase that is expressed during early development

Authors:

Devader C, Webb RJ, Thomas GM, Dale L.

Journal:

Gene (2006): 135

Immunoassay for B. globigii spores as a model for detecting B. anthracis spores in finished water

Authors:

Farrell S, Halsall HB, Heineman WR.

Journal:

Analyst (2005): 489

Controlled initiation of enzymatic reactions in micrometer-sized biomimetic compartments

Authors:

Karlsson A, Sott K, Markstrom M, Davidson M, Konkoli Z, Orwar O.

Journal:

J Phys Chem B Condens Matter Mater Surf Interfaces Biophys (2005): 1609

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C)
UNSPSC	12171501

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Page updated on October 9, 2025