Signal Guard™ phosphatase reaction stopping solution

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C)
UNSPSC	12171501

Overview SDS Protocol

Example protocol

SAMPLE EXPERIMENTAL PROTOCOL

Assay Protocol for one 96-well plate

Warm the Signal Guard™ phosphatase reaction stopping solution to room temperature.
At the desired stopping time point, add 50 µL of Signal Guard™ phosphatase reaction stopping solution per 200 µL volume in each microplate well. Note: For other reaction volumes, adjust the addition of Signal Guard™ Phosphatase reaction stopping solution proportionally (e.g. add 5 µL to a 25 µL reaction volume). The signal level should remain stable for at least 18 hours.

Images

Figure 1. The application of Signal Guard™ phosphatase reaction stopping solution in Acid phosphatase fluorescence (AAT Bioquest Cat#11627) assay. Two parallel reactions containing 100 mU/mL Acid phosphatase was initiated by adding 200 μL reaction mixture. Reactions were incubated at room temperature for times indicated above and then 50 µL Signal Guard™ phosphatase reaction stopping solution was added to one reaction. The plots demonstrated that the reaction is completely inhibited by Signal Guard™ phosphatase reaction stopping solution.

Figure 2. The application of Signal Guard™ phosphatase reaction stopping solution in Alkaline phosphatase colorimetric assay (AAT Bioquest Cat#11950). Two parallel reactions containing 100 mU/mL Alkaline phosphatase was initiated by adding 200 μL reaction mixture. Reactions were incubated at room temperature for times indicated above and then 50 µL Signal Guard™ phosphatase reaction stopping solution was added to one reaction. The plots demonstrated that the reaction is completely inhibited by Signal Guard™ phosphatase reaction stopping solution.

Citations

View all 104 citations: Citation Explorer

Discoveries of the phosphatidate phosphatase genes in yeast published in the Journal of Biological Chemistry
Authors: Carman, G. M.
Journal: J Biol Chem (2019): 1681-1689

Structure and functions of His domain protein tyrosine phosphatase in receptor trafficking and cancer (1)
Authors: Desrochers, G., Kazan, J. M., Pause, A.
Journal: Biochem Cell Biol (2019): 68-72

Alkaline phosphatase: a potential biomarker for stroke and implications for treatment
Authors: Brichacek, A. L., Brown, C. M.
Journal: Metab Brain Dis (2019): 3-19

Regulation of hematopoietic cell signaling by SHIP-1 inositol phosphatase: growth factors and beyond
Authors: Hibbs, M. L., Raftery, A. L., Tsantikos, E.
Journal: Growth Factors (2019): 1-19

Fat-regulating phosphatidic acid phosphatase: a review of its roles and regulation in lipid homeostasis
Authors: Carman, G. M., Han, G. S.
Journal: J Lipid Res (2019): 2-6

Roles of phosphatase and tensin homolog in skeletal muscle
Authors: Shan, T., Liu, J., Xu, Z., Wang, Y.
Journal: J Cell Physiol (2019): 3192-3196

Myosin phosphatase: Unexpected functions of a long-known enzyme
Authors: Kiss, A., Erdodi, F., Lontay, B.
Journal: Biochim Biophys Acta Mol Cell Res (2019): 2-15

Redox inhibition of protein phosphatase PP2A: Potential implications in oncogenesis and its progression
Authors: Raman, D., Pervaiz, S.
Journal: Redox Biol (2019): 101105

Dynamic structural rearrangements and functional regulation of voltage-sensing phosphatase
Authors: Sakata, S., Okamura, Y.
Journal: J Physiol (2019): 29-40

Tuning the Protein Phosphorylation by Receptor Type Protein Tyrosine Phosphatase Epsilon (PTPRE) in Normal and Cancer Cells
Authors: Liang, J., Shi, J., Wang, N., Zhao, H., Sun, J.
Journal: J Cancer (2019): 105-111

References

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Journal: Biochemistry (2006): 3460

Xenopus apyrase (xapy), a secreted nucleotidase that is expressed during early development
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Authors: Montalibet J, Skorey KI, Kennedy BP.
Journal: Methods (2005): 2

Assaying Cdc25 phosphatase activity
Authors: Hassepass I, Hoffmann I.
Journal: Methods Mol Biol (2004): 153

FITC binding site and p-nitrophenyl phosphatase activity of the Kdp-ATPase of Escherichia coli
Authors: Bramkamp M, Gassel M, Altendorf K.
Journal: Biochemistry (2004): 4559

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Measuring the specific activity of the CD45 protein tyrosine phosphatase
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Journal: J Immunol Methods (2003): 127