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AAT Bioquest

Signal Guard™ phosphatase reaction stopping solution

The application of Signal Guard™ phosphatase reaction stopping solution in Acid phosphatase fluorescence (AAT Bioquest Cat#11627) assay. Two parallel reactions containing 100 mU/mL Acid phosphatase was initiated by adding 200 μL reaction mixture. Reactions were incubated at room temperature for times indicated above and then 50 µL Signal Guard™ phosphatase reaction stopping solution was added to one reaction. The plots demonstrated that the reaction is completely inhibited by Signal Guard™ phosphatase reaction stopping solution.
The application of Signal Guard™ phosphatase reaction stopping solution in Acid phosphatase fluorescence (AAT Bioquest Cat#11627) assay. Two parallel reactions containing 100 mU/mL Acid phosphatase was initiated by adding 200 μL reaction mixture. Reactions were incubated at room temperature for times indicated above and then 50 µL Signal Guard™ phosphatase reaction stopping solution was added to one reaction. The plots demonstrated that the reaction is completely inhibited by Signal Guard™ phosphatase reaction stopping solution.
The application of Signal Guard™ phosphatase reaction stopping solution in Acid phosphatase fluorescence (AAT Bioquest Cat#11627) assay. Two parallel reactions containing 100 mU/mL Acid phosphatase was initiated by adding 200 μL reaction mixture. Reactions were incubated at room temperature for times indicated above and then 50 µL Signal Guard™ phosphatase reaction stopping solution was added to one reaction. The plots demonstrated that the reaction is completely inhibited by Signal Guard™ phosphatase reaction stopping solution.
The application of Signal Guard™ phosphatase reaction stopping solution in Alkaline phosphatase colorimetric assay (AAT Bioquest Cat#11950). Two parallel reactions containing 100 mU/mL Alkaline phosphatase was initiated by adding 200 μL reaction mixture. Reactions were incubated at room temperature for times indicated above and then 50 µL Signal Guard™ phosphatase reaction stopping solution was added to one reaction. The plots demonstrated that the reaction is completely inhibited by Signal Guard™ phosphatase reaction stopping solution.
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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C)
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Alkaline and acid phosphatases are the hydrolase enzymes responsible for dephosphorylation of molecules such as nucleotides, proteins and alkaloids under alkaline and acidic conditions, respectively. Some phosphatases assays and tests require the phosphatase reactions to be stopped for further analysis using the stop solutions. Our Signal Guard™ phosphatase reaction stopping solution is a ready to use reagent that provides a convenient tool for terminating fluorescence and colorimetric signal-generating phosphatase reactions at a user-determined time point, and also keep the fluorescence or colorimetric signal stable for up to 18 hours. The Signal Guard™ phosphatase reaction stopping solution is optimized and compatible with alkaline, acid and protein phosphatase. Compared to other commercial phosphatase stopping reagents, our Signal Guard™ phosphatases reaction stopping solution is nearly neutral and mild. It is compatible with a vast majority of colorimetric and fluorimetric phosphatase substrates while the stop reagents from other vendors are not compatible with most of fluorescence-based phosphatase assays due to their extremely high pH.

Example protocol


SAMPLE EXPERIMENTAL PROTOCOL

Assay Protocol for one 96-well plate

  1. Warm the Signal Guard™ phosphatase reaction stopping solution to room temperature.

  2. At the desired stopping time point, add 50 µL of Signal Guard™ phosphatase reaction stopping solution per 200 µL volume in each microplate well. Note: For other reaction volumes, adjust the addition of Signal Guard™ Phosphatase reaction stopping solution proportionally (e.g. add 5 µL to a 25 µL reaction volume). The signal level should remain stable for at least 18 hours.

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Citations


View all 104 citations: Citation Explorer
Discoveries of the phosphatidate phosphatase genes in yeast published in the Journal of Biological Chemistry
Authors: Carman, G. M.
Journal: J Biol Chem (2019): 1681-1689
Structure and functions of His domain protein tyrosine phosphatase in receptor trafficking and cancer (1)
Authors: Desrochers, G., Kazan, J. M., Pause, A.
Journal: Biochem Cell Biol (2019): 68-72
Alkaline phosphatase: a potential biomarker for stroke and implications for treatment
Authors: Brichacek, A. L., Brown, C. M.
Journal: Metab Brain Dis (2019): 3-19
Regulation of hematopoietic cell signaling by SHIP-1 inositol phosphatase: growth factors and beyond
Authors: Hibbs, M. L., Raftery, A. L., Tsantikos, E.
Journal: Growth Factors (2019): 1-19
Fat-regulating phosphatidic acid phosphatase: a review of its roles and regulation in lipid homeostasis
Authors: Carman, G. M., Han, G. S.
Journal: J Lipid Res (2019): 2-6
Roles of phosphatase and tensin homolog in skeletal muscle
Authors: Shan, T., Liu, J., Xu, Z., Wang, Y.
Journal: J Cell Physiol (2019): 3192-3196
Myosin phosphatase: Unexpected functions of a long-known enzyme
Authors: Kiss, A., Erdodi, F., Lontay, B.
Journal: Biochim Biophys Acta Mol Cell Res (2019): 2-15
Redox inhibition of protein phosphatase PP2A: Potential implications in oncogenesis and its progression
Authors: Raman, D., Pervaiz, S.
Journal: Redox Biol (2019): 101105
Dynamic structural rearrangements and functional regulation of voltage-sensing phosphatase
Authors: Sakata, S., Okamura, Y.
Journal: J Physiol (2019): 29-40
Tuning the Protein Phosphorylation by Receptor Type Protein Tyrosine Phosphatase Epsilon (PTPRE) in Normal and Cancer Cells
Authors: Liang, J., Shi, J., Wang, N., Zhao, H., Sun, J.
Journal: J Cancer (2019): 105-111

References


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Journal: Anal Chem (2006): 7022
TNP-8N3-ADP photoaffinity labeling of two Na,K-ATPase sequences under separate Na+ plus K+ control
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Xenopus apyrase (xapy), a secreted nucleotidase that is expressed during early development
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Journal: Gene (2006): 135
Immunoassay for B. globigii spores as a model for detecting B. anthracis spores in finished water
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Journal: Analyst (2005): 489
Controlled initiation of enzymatic reactions in micrometer-sized biomimetic compartments
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Protein tyrosine phosphatase: enzymatic assays
Authors: Montalibet J, Skorey KI, Kennedy BP.
Journal: Methods (2005): 2
Assaying Cdc25 phosphatase activity
Authors: Hassepass I, Hoffmann I.
Journal: Methods Mol Biol (2004): 153
FITC binding site and p-nitrophenyl phosphatase activity of the Kdp-ATPase of Escherichia coli
Authors: Bramkamp M, Gassel M, Altendorf K.
Journal: Biochemistry (2004): 4559
Flow injection analysis in a microfluidic format
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Journal: Anal Chem (2003): 967
Measuring the specific activity of the CD45 protein tyrosine phosphatase
Authors: Rider DA, Young SP.
Journal: J Immunol Methods (2003): 127